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    • 8. 发明申请
    • CELLS AND METHODS FOR PRODUCING LUTEIN
    • 用于生产罗廷辛的细胞和方法
    • WO2013119552A3
    • 2015-06-18
    • PCT/US2013024746
    • 2013-02-05
    • UNIV CITY NEW YORK RES FOUND
    • WURTZEL ELEANORE TQUINLAN RENA
    • C07C49/00C07C35/21C12P23/00
    • C12P23/00A23L33/135A23L33/145C07C403/24C12N9/001C12N9/0073C12N9/0083C12N9/1085C12N9/90C12Y103/05006C12Y114/13129C12Y114/99045C12Y205/01029C12Y205/01032C12Y505/01018C12Y505/01019
    • Provided herein are recombinant cells (e.g., recombinant bacteria or plant, insect, mammalian, and yeast cells) containing a nucleic acid encoding a CYP97A protein or a nucleic acid encoding a CYP97B protein; a nucleic acid encoding a CYP97C protein; a nucleic acid encoding a geranylgeranyl pyrophosphate synthase protein; a nucleic acid encoding a phytoene synthase protein; a nucleic acid encoding a phytoene desaturase protein; a nucleic acid encoding a lycopene β-cyclase protein; and a nucleic acid encoding a lycopene ε-cyclase protein. Also provided are methods of producing lutein that include culturing these recombinant cells (e.g., recombinant bacteria and yeast cells), and methods of generating these recombinant cells (e.g., recombinant bacteria and yeast cells). Also provided is lutein produced by these methods, and pharmaceutical compositions, food supplements, food products, and cosmetic compositions that contain lutein produced by these methods.
    • 本文提供了含有编码CYP97A蛋白的核酸或编码CYP97B蛋白的核酸的重组细胞(例如重组细菌或植物,昆虫,哺乳动物和酵母细胞) 编码CYP97C蛋白质的核酸; 编码香叶基香叶基焦磷酸合酶蛋白的核酸; 编码芸苔属合酶蛋白的核酸; 编码八氢番茄红素去饱和酶蛋白的核酸; 编码番茄红素β-环蛋白的核酸; 和编码番茄红素ε-环化酶蛋白质的核酸。 还提供了生产包括培养这些重组细胞(例如重组细菌和酵母细胞)的叶黄素的方法以及产生这些重组细胞(例如重组细菌和酵母细胞)的方法。 还提供了通过这些方法生产的叶黄素,以及含有通过这些方法生产的叶黄素的药物组合物,食品添加剂,食品和化妆品组合物。