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    • 3. 发明公开
    • Method for preparing cellobiose
    • Verfahren zur Herstellung von Cellobiose。
    • EP0423768A1
    • 1991-04-24
    • EP90119930.7
    • 1990-10-17
    • Umeda, Keiji, Director-General of National Food Research Institute,NIPPON PETROCHEMICALS COMPANY, LIMITED
    • Taniguchi, HajimeSasaki, TakashiKitaoka, Motomitsu
    • C12P19/12C12P19/24
    • C12P19/24C12P19/12
    • A method for preparing cellobiose from sucrose in a high yield at low cost without difficulty by using three enzymes of sucrose phosphorylase, glucose isomerase and cellobiose phosphorylase in the presence of orthophosphate. The additional method of the invention comprises 4 steps: to treat sucrose with sucrose phosphorylase in the presence of orthophosphate to produce fructose and glucose-1-phosphate; to treat the fructose obtained in the preceding step with glucose isomerase to produce glucose; to treat the glucose obtained in the preceding step and the glucose-1-phosphate obtained in the first step with cellobiose phosphorylase to produce cellobiose and orthophosphate; and to recover at least a part of cellobiose from the reaction mixture in the preceding step and to recycle at least a part of the remaining reaction mixtue containing orthophosphate to the first step.
    • 在正磷酸盐存在的情况下,通过使用蔗糖磷酸化酶,葡萄糖异构酶和纤维二糖磷酸化酶三种酶难以低成本从蔗糖制备纤维二糖的方法。 本发明的另外的方法包括4个步骤:在正磷酸盐存在下用蔗糖磷酸化酶处理蔗糖以产生果糖和葡萄糖-1-磷酸酯; 用葡萄糖异构酶处理在前一步骤中获得的果糖以产生葡萄糖; 将上述步骤中得到的葡萄糖和第一步得到的葡萄糖-1-磷酸酯用纤维二糖磷酸化酶处理以产生纤维二糖和正磷酸盐; 并从前述步骤中的反应混合物中回收至少一部分纤维二糖,并将含有正磷酸盐的剩余反应混合物的至少一部分循环至第一步骤。