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1. 发明授权

US08277820B2 Process for preparing variant of Erysipelothrix rhusiopathiae surface protective antigen in E. coli 有权
标题翻译：在大肠杆菌中制备欧洲白喉病毒性表皮保护性抗原变异体的方法
公开(公告)号：US08277820B2
公开(公告)日：2012-10-02
申请号：US10590896
申请日：2005-02-08
申请人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
发明人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
IPC分类号： A61K39/00 , A61K39/38 , A61K39/02 , C12P21/06 , C12N15/09
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译：提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。

2. 发明申请

US20080286309A1 Process for Preparing Variant of Erysipelothrix Rhusiopathiae Surface Protective Antigen in E. Coli 有权
标题翻译：大肠杆菌表皮保护性抗原的制备方法
公开(公告)号：US20080286309A1
公开(公告)日：2008-11-20
申请号：US10590896
申请日：2005-02-08
申请人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
发明人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
IPC分类号： A61K39/02 , C12P21/00 , C12N15/31 , C07K14/195
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译：提供了Erysillolothrix rhusiopathiae表面保护性抗原SpaA蛋白质或缩短形式的SpaA（DeltaSpaA）的变体，其中一部分SpaA蛋白质被缺失以防止欧洲白喉病毒性鼻炎病毒感染及其制备方法。在SpaA或DeltaSpaA蛋白的氨基酸序列中的特定位点处引入氨基酸取代提供了SPAA或DeltaSpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中作为包涵体表达。本发明的SpaA或DeltaSpaA蛋白的变体可以容易地被回收和纯化，因为它作为包涵体在大肠杆菌中表达。

3. 发明授权

US08361749B2 Process for preparing variant of Erysipelothrix rhusiopathiae surface protective antigen in E. coli 有权
标题翻译：在大肠杆菌中制备欧洲白喉病毒性表皮保护性抗原变异体的方法
公开(公告)号：US08361749B2
公开(公告)日：2013-01-29
申请号：US12949606
申请日：2010-11-18
申请人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
发明人： Toshihiro Ushijima , Masashi Sakaguchi , Eiji Tokunaga
IPC分类号： A61K39/00 , A61K39/38 , A61K39/02 , C12P21/06 , C12N15/00
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译：提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。

4. 发明授权

US08298778B2 Method and kit for detecting antibody to avibacterium paragallinarum 有权
标题翻译：用于检测抗杆菌杆菌抗体的方法和试剂盒
公开(公告)号：US08298778B2
公开(公告)日：2012-10-30
申请号：US12866793
申请日：2009-02-06
申请人： Toshihiro Ushijima , Takashi Imamura , Ryuichi Sakamoto , Masashi Sakaguchi
发明人： Toshihiro Ushijima , Takashi Imamura , Ryuichi Sakamoto , Masashi Sakaguchi
IPC分类号： C07K1/00 , C07K2/00 , G01N33/53 , G01N33/569
CPC分类号： C07K16/1242 , C07K14/195 , C07K2317/23 , G01N33/56911 , G01N2469/20 , G01N2800/14
摘要： A method and a kit for detecting an antibody to Avibacterium paragallinarum are provided. A method for detecting an antibody to Avibacterium paragallinarum which comprises detecting an antibody induced by an outer-membrane protein of Avibacterium paragallinarum serotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.
摘要翻译：提供了用于检测副杆菌的抗体的方法和试剂盒。一种检测副杆菌的抗体的方法，其特征在于，检测由Pariballinarum血清型A和/或血清型C的外膜蛋白诱导的抗体，所述抗体通过ELISA与固相相结合，所述固相由非氨基酸序列的氨基酸序列组成，所述外膜蛋白或其部分的同源区域被固定化，以及用于所述方法的检测试剂盒。

5. 发明申请

US20110201034A1 METHOD AND KIT FOR DETECTING ANTIBODY TO AVIBACTERIUM PARAGALLINARUM 有权
标题翻译：用于检测抗菌药物的方法和试剂盒
公开(公告)号：US20110201034A1
公开(公告)日：2011-08-18
申请号：US12866793
申请日：2009-02-06
申请人： Toshihiro Ushijima , Takashi Imamura , Ryuichi Sakamoto , Masashi Sakaguchi
发明人： Toshihiro Ushijima , Takashi Imamura , Ryuichi Sakamoto , Masashi Sakaguchi
IPC分类号： G01N33/566 , C07K7/06 , C07K7/08
CPC分类号： C07K16/1242 , C07K14/195 , C07K2317/23 , G01N33/56911 , G01N2469/20 , G01N2800/14
摘要： A method and a kit for detecting an antibody to Avibacterium paragallinarum are provided. A method for detecting an antibody to Avibacterium paragallinarum which comprises detecting an antibody induced by an outer-membrane protein of Avibacterium paragallinarum serotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.
摘要翻译：提供了用于检测副杆菌的抗体的方法和试剂盒。一种检测副杆菌的抗体的方法，其特征在于，检测由Pariballinarum血清型A和/或血清型C的外膜蛋白诱导的抗体，所述抗体通过ELISA与固相相结合，所述固相由非氨基酸序列的氨基酸序列组成，所述外膜蛋白或其部分的同源区域被固定化，以及用于所述方法的检测试剂盒。

6. 发明申请

US20110059021A1 PROCESS FOR PREPARING VARIANT OF ERYSIPELOTHRIX RHUSIOPATHIAE SURFACE PROTECTIVE ANTIGEN IN E. COLI 有权
标题翻译：在大肠杆菌中制备抗坏血酸表面抗原的变体的方法
公开(公告)号：US20110059021A1
公开(公告)日：2011-03-10
申请号：US12949606
申请日：2010-11-18
申请人： Toshihiro USHIJIMA , Masashi Sakaguchi , Eiji Tokunaga
发明人： Toshihiro USHIJIMA , Masashi Sakaguchi , Eiji Tokunaga
IPC分类号： A61K49/00 , C12P21/00 , A61P37/04 , A61P31/04
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译：提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。

7. 发明授权

US06909184B2 TAB type semiconductor device 失效
标题翻译： TAB型半导体器件
公开(公告)号：US06909184B2
公开(公告)日：2005-06-21
申请号：US10278272
申请日：2002-10-22
申请人： Toshihiro Ushijima , Isao Baba , Takamitsu Sumiyoshi
发明人： Toshihiro Ushijima , Isao Baba , Takamitsu Sumiyoshi
IPC分类号： H01L23/13 , H01L23/498 , H05K1/02 , H05K1/11 , H01L23/48 , H01L23/52 , H01L29/40
CPC分类号： H01L23/13 , H01L23/49816 , H01L23/49838 , H01L23/4985 , H01L24/45 , H01L24/48 , H01L24/49 , H01L2224/32225 , H01L2224/45144 , H01L2224/4824 , H01L2224/49175 , H01L2224/73215 , H01L2924/00014 , H01L2924/01079 , H01L2924/14 , H01L2924/15311 , H01L2924/3025 , H05K1/0271 , H05K1/111 , H01L2924/00 , H01L2224/45015 , H01L2924/207
摘要： There is disclosed a TAB style BGA type semiconductor device. This semiconductor device comprises a semiconductor chip on which an integrated circuit is formed, and a polyimide tape which has a conductive pattern and which is allowed to adhere to the semiconductor chip. The conductive pattern includes a bonding portion connected to the pad of the semiconductor chip, a pad portion connected to the outside electrode, and an electrically floating island-like portion in addition to a wiring portion for connecting the bonding portion and the pad portion.
摘要翻译：公开了一种TAB型BGA型半导体器件。该半导体器件包括其上形成集成电路的半导体芯片和具有导电图案并允许粘附到半导体芯片的聚酰亚胺胶带。导电图案包括连接到半导体芯片的焊盘的接合部分，连接到外部电极的焊盘部分，以及除了用于连接接合部分和焊盘部分的布线部分之外的电浮动岛状部分。

8. 发明授权

US6124432A Process for purifying dermonecrotic toxin produced by Bordetella and toxoid 失效
标题翻译：用于纯化由博德特氏菌和类毒素产生的皮肤坏死毒素的方法
公开(公告)号：US6124432A
公开(公告)日：2000-09-26
申请号：US750166
申请日：1996-12-02
申请人： Toru Kawai , Toshihiro Ushijima , Kozo Takase , Hideo Fujikawa
发明人： Toru Kawai , Toshihiro Ushijima , Kozo Takase , Hideo Fujikawa
IPC分类号： A61K39/00 , A61P31/04 , C07K14/235 , C07K14/285 , C07K1/00 , A23J1/00 , C07K14/00 , C07K16/00
CPC分类号： C07K14/285 , C07K14/235 , A61K39/00
摘要： The present invention aims at providing a method for preparing dermonecrotic toxin, an improved toxoid of dermonecrotic toxin produced by Bordetelia and a toxoid mixture comprising said improved toxoid of dermonecrotic toxin produced by Bordetella and a toxoid of dermonecrotic toxin produced by Pasteurella. There is provided a method for partially purifying dermonecrotic toxin which comprises bringing a dermonecrotic toxin-containing solution into contact with a chromatographic gel sulfated by direct sulfation or a chromatographic gel to which a sulfated molecule is covalently bonded to thereby make the dermonecrotic toxin adsorbed on the gel, and eluting the adsorbed dermonecrotic toxin from the gel. There is also provided a toxoid obtained from dermonecrotic toxin produced by Bordetella, said dermonecrotic toxin being prepared by the above method for partially purifying dermonecrotic toxin, and a toxoid mixture prepared by mixing said toxoid and a toxoid of dermonecrotic toxin produced by toxigenic Pasteurella, said toxoid mixture being safe and effective for preventing the atrophic rhinitis in pigs.
摘要翻译： PCT No.PCT / JP95 / 01125 Sec。 371日期1996年12月2日第 102（e）日期1996年12月2日PCT提交1995年6月7日PCT公布。公开号WO95 / 34322 日期1995年12月21日本发明旨在提供一种制备皮肤坏死毒素的方法，由博德特利氏菌产生的改良的皮肤坏死毒素类毒素，以及由博德特氏菌产生的所述改良的皮肤坏死毒素类毒素和由巴斯德氏菌产生的皮肤坏死毒素类毒素的类毒素混合物。提供了部分纯化皮肤坏死毒素的方法，其包括将含有皮肤坏死毒素的溶液与通过直接硫酸化硫酸化的色谱凝胶接触，或者将硫酸化分子共价键合到色谱凝胶上，从而使皮肤坏死毒素吸附在凝胶，并从凝胶中洗脱吸附的皮肤坏死毒素。还提供了由博德特氏菌产生的皮肤坏死毒素获得的类毒素，所述皮肤坏死毒素通过上述部分纯化皮肤坏死毒素的方法制备，以及通过混合所述类毒素和由产毒巴斯德氏菌产生的皮肤坏死毒素类毒素制备的类毒素混合物，类毒素混合物对于预防猪萎缩性鼻炎是安全有效的。

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