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1. 发明授权

US5856092A Detection of a nucleic acid sequence or a change therein 失效
标题翻译：检测核酸序列或其中的变化
公开(公告)号：US5856092A
公开(公告)日：1999-01-05
申请号：US429659
申请日：1995-04-27
申请人： James Langham Dale , Peter Timms , Terence Patrick Walsh
发明人： James Langham Dale , Peter Timms , Terence Patrick Walsh
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/6827
摘要： A method for detecting a specific polynucleotide sequence in a material is disclosed. The method includes exposing said material to an oligonucleotide primer having a sequence complementary to part of said specific polynucleotide sequence wherein said primer binds to part of said polynucleotide sequence when present in said material. Primer bound to the polynucleotide sequence is extended wherein any extended primer includes a detectable element and/or a separation element. Any extended primer is then separated into a fraction wherein said fraction does not have detectable element not included in said extended primer. One then determines whether any extended primer is present in said fraction by assaying said fraction for said extended primer wherein the presence of said extended primer is indicative of the presence of the specific polynucleotide sequence in said material and the absence of said extended primer is indicative of the presence of the specific polynucleotide sequences in said material and the absence of said extended primer in said fraction is indicative of the absence of the specific polynucleotide sequence in said material.
摘要翻译：公开了一种用于检测材料中特定多核苷酸序列的方法。该方法包括将所述材料暴露于具有与所述特异性多核苷酸序列的一部分互补的序列的寡核苷酸引物，其中当存在于所述材料中时，所述引物与部分所述多核苷酸序列结合。与多核苷酸序列结合的引物延伸，其中任何延伸的引物包括可检测元件和/或分离元件。然后将任何延伸的引物分离成其中所述级分不具有未包含在所述延伸引物中的可检测元件的级分。然后通过测定所述延伸引物的所述级分来确定所述级分中是否存在任何延伸引物，其中所述延伸引物的存在指示所述材料中特异性多核苷酸序列的存在，并且所述延伸引物不存在指示所述材料中特定多核苷酸序列的存在和所述级分中不存在所述延伸引物指示所述材料中不存在特异性多核苷酸序列。

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