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    • 6. 发明授权
    • Method for assessing proteolytic enzyme activity using ubiquitin fusion substrate
    • 使用泛素融合底物评估蛋白水解酶活性的方法
    • US07842460B2
    • 2010-11-30
    • US11156707
    • 2005-06-21
    • Tauseef R. ButtAlejandro Bernal
    • Tauseef R. ButtAlejandro Bernal
    • C12Q1/37C12N9/48G01N33/53G01N33/537
    • G01N33/573C12Q1/37G01N2333/916
    • Methods and kits for assessing proteolytic enzyme activity and a modulator's effect thereof employ a Ubiquitin or Ubiquitin-like Protein and a signal producing structure. The corresponding fusion polynucleotide is employed for production of transgenic cells, plants and animals that may be produced by stably transfection, optionally transforming the cell, plant or animal with a Ubiquitin-, UBL- or their C-terminal binding functional fragment-Reporter fusion polynucleotide. A method of diagnosing a disease or condition, comprises contacting or administering a sample obtained from a subject suspected of being afflicted with the disease or condition with the cell, plant or animal, detecting any signal produced by the reporter in the presence of the sample and comparing the signal to controls for 0% and 100% signals.
    • 用于评估蛋白水解酶活性的方法和试剂盒及其调节剂的作用采用泛素或泛素样蛋白和信号产生结构。 相应的融合多核苷酸用于生产可通过稳定转染产生的转基因细胞,植物和动物,任选地用泛素,UBL-或其C-末端结合功能片段 - 报道融合多核苷酸转化细胞,植物或动物 。 一种诊断疾病或病症的方法包括将从疑似患有疾病或病症的受试者获得的样品与细胞,植物或动物接触或施用,在样品存在下检测报告物产生的任何信号,以及 将信号与0%和100%信号的控制进行比较。