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1. 发明授权

US08148510B2 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US08148510B2
公开(公告)日：2012-04-03
申请号：US12795770
申请日：2010-06-08
申请人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Natalia Ellanskaya, legal representative , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： C12N15/82 , A01H1/00
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

2. 发明授权

US07306946B2 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US07306946B2
公开(公告)日：2007-12-11
申请号：US11174413
申请日：2005-07-01
申请人： Daniel J. Altier , Glen Dahlbacka , Natalia Ellanskaya, legal representative , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Irina Ellanskaya, deceased
IPC分类号： C12N15/82 , C12N5/10 , C12N15/74 , C07H21/04
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

3. 发明授权

US08753844B2 Overproduction of ligninolytic enzymes 有权
标题翻译：木质素分解酶过度生产
公开(公告)号：US08753844B2
公开(公告)日：2014-06-17
申请号：US13464681
申请日：2012-05-04
申请人： Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
发明人： Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
IPC分类号： C12P1/02 , C12N1/14 , C12N9/02
CPC分类号： C12N9/248 , C12N9/0061 , C12N9/0065 , C12N9/2437 , C12P21/00 , C12P39/00 , C12P2203/00 , C12Y110/03002 , C12Y111/01013
摘要： Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IBB 303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.
摘要翻译：本文描述了用于从basidiomycetous真菌过度产生木质素分解酶的方法，组合物和系统。如上所述，该方法可以包括在具有包含木质纤维素材料的生长培养基的发酵系统中温育真菌菌株Cerrena unicolor IBB 303，然后在真菌表达木质素分解酶的条件下培养发酵系统中的真菌菌株。在一些情况下，木质纤维素材料是普通话剥离，乙醇生产残留物，核桃果皮，麦麸，小麦秸秆或香蕉皮。

4. 发明授权

US5401630A Species-specific DNA-DNA hybridization probe prepared using chromosome size DNA 失效
标题翻译：使用染色体大小DNA制备的物种特异性DNA-DNA杂交探针
公开(公告)号：US5401630A
公开(公告)日：1995-03-28
申请号：US695167
申请日：1991-05-03
申请人： Tamas Torok , A. Douglas King, Jr. , David R. Rockhold , Christina Royer
发明人： Tamas Torok , A. Douglas King, Jr. , David R. Rockhold , Christina Royer
IPC分类号： C12Q1/68 , C07H23/00 , C12P19/34 , C12Q1/00
CPC分类号： C12Q1/6893 , C12Q1/6858 , C12Q1/6895
摘要： A species-specific DNA-DNA hybridization probe prepared using an isolated whole chromosome of a lower eukaryote as template. The probe is useful for the detection of conspecificity in lower eukaryotes selected from the group consisting of yeast and molds.
摘要翻译：使用低等真核生物的分离的整个染色体作为模板制备的物种特异性DNA-DNA杂交探针。探针可用于检测选自酵母和霉菌的低等真核生物的特异性。

5. 发明申请

US20120295324A1 OVERPRODUCTION OF LIGNINOLYTIC ENZYMES 有权
标题翻译：丝氨酸酶的超导
公开(公告)号：US20120295324A1
公开(公告)日：2012-11-22
申请号：US13464681
申请日：2012-05-04
申请人： Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
发明人： Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
IPC分类号： C12N9/02 , C12M1/02
CPC分类号： C12N9/248 , C12N9/0061 , C12N9/0065 , C12N9/2437 , C12P21/00 , C12P39/00 , C12P2203/00 , C12Y110/03002 , C12Y111/01013
摘要： Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IB303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.
摘要翻译：本文描述了用于从basidiomycetous真菌过度产生木质素分解酶的方法，组合物和系统。如上所述，该方法可以包括在具有包含木质纤维素材料的生长培养基的发酵系统中温育真菌菌株Cerrena unicolor IB303，然后在真菌表达木质素分解酶的条件下培养发酵系统中的真菌菌株。在一些情况下，木质纤维素材料是普通话剥离，乙醇生产残留物，核桃果皮，麦麸，小麦秸秆或香蕉皮。

6. 发明授权

US07923599B2 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US07923599B2
公开(公告)日：2011-04-12
申请号：US11833535
申请日：2007-08-03
申请人： Daniel J. Altier , Glen Dahlbacka , Irina Elleskaya , Natalia Ellanskaya, legal representative , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Glen Dahlbacka , Irina Elleskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

7. 发明申请

US20100303762A1 Antifungal Polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US20100303762A1
公开(公告)日：2010-12-02
申请号：US12795770
申请日：2010-06-08
申请人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Natalia Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Natalia Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： A01N63/00 , C07H21/04 , C12N15/63 , C12N5/10 , A01P3/00
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

8. 发明申请

US20090264351A1 ANTIFUNGAL POLYPEPTIDES 有权
标题翻译：抗菌多糖
公开(公告)号：US20090264351A1
公开(公告)日：2009-10-22
申请号：US11833598
申请日：2007-08-03
申请人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Natalia Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Glen Dahlbacka , Irina Ellanskaya , Natalia Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy F. McCutchen , James K. Presnail , Janet A. Rice , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： A01N37/18 , C07K14/00 , A01P3/00
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

9. 发明授权

US07589176B2 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US07589176B2
公开(公告)日：2009-09-15
申请号：US11657710
申请日：2007-01-24
申请人： Daniel J. Altier , Irina Ellanskaya , Natalia Ellanskaya, legal representative , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Irina Ellanskaya , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： C07K14/00 , C07K14/415 , C07K17/00
CPC分类号： C12N15/8282 , A01N37/46 , A01N63/04 , C07K14/37
摘要： The invention relates to antifungal compositions and methods for protecting a plant from a fungal pathogen. Compositions including antifungal polypeptides isolated from a fungal fermentation broth are provided.
摘要翻译：本发明涉及用于保护植物免受真菌病原体的抗真菌组合物和方法。提供包括从真菌发酵液中分离的抗真菌多肽的组合物。

10. 发明申请

US20060031962A1 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US20060031962A1
公开(公告)日：2006-02-09
申请号：US11174413
申请日：2005-07-01
申请人： Daniel Altier , Glen Dahlbacka , I.A. Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy McCutchen , James Presnail , Janet Rice , Eric Schepers , Carl Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel Altier , Glen Dahlbacka , I.A. Ellanskaya , Rafael Herrmann , Jennie Hunter-Cevera , Billy McCutchen , James Presnail , Janet Rice , Eric Schepers , Carl Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： A61K38/17 , A01H1/00 , C07H21/04 , C12N5/04 , C07K14/415 , C12N15/82
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

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