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1. 发明授权

US5308769A Cancer-related human galactosyltransferase GT-II 失效
标题翻译：癌症相关人类半乳糖基转移酶GT-II
公开(公告)号：US5308769A
公开(公告)日：1994-05-03
申请号：US911273
申请日：1992-07-09
申请人： Morito Uemura , Shinya Yoshida , Takao Uejima
发明人： Morito Uemura , Shinya Yoshida , Takao Uejima
IPC分类号： C07K16/40 , C12N9/10 , A61K39/00
CPC分类号： C12N9/1051 , C07K16/40 , Y10S435/815
摘要： A substantially purified cancer-related galactosyltransferase and a monoclonal antibody which may be used as a diagnostic reagent of cancer are disclosed. The galactosyltransferase of the present invention has a molecular weight of about 50,000 determined by SDS-polyacrylamide gel electrophoresis under reducing conditions and has a reactivity with MAb4880 under native conditions. A part of the galactosyltransferase self-associates and its reactivity with MAb4880 and the degree of self-association are promoted when it is reduced so that a part of which is detected in a fraction of a molecular weight of not less than 200,000 in gel permeation chromatography. The galactosyltransferase of the present invention can be used as a cancer marker or as a reagent for preparing a monoclonal antibody with a specificity to the galactosyltransferase, which monoclonal antibody can be used as a diagnostic of a cancer. The monoclonal antibody of the invention specifically reacts with the cancer-related galactosyltransferase of the invention.
摘要翻译：公开了可用作癌症诊断试剂的基本上纯化的癌相关半乳糖基转移酶和单克隆抗体。本发明的半乳糖基转移酶在还原条件下通过SDS-聚丙烯酰胺凝胶电泳测定的分子量约为50,000，并且在天然条件下具有与MAb4880的反应性。一部分半乳糖基转移酶自身缔合物及其与MAb4880的反应性和自身结合程度在减少时促进其部分在凝胶渗透色谱法中以分子量不低于20万的分数检测。本发明的半乳糖基转移酶可以用作癌症标记物或用作制备对半乳糖基转移酶特异性的单克隆抗体的试剂，该单克隆抗体可以用作癌症的诊断。本发明的单克隆抗体与本发明的与癌症相关的半乳糖基转移酶特异性地反应。

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