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1. 发明申请

WO2012158296A1 METHODS TO INCREASE PLANT PRODUCTIVITY 审中-公开
标题翻译：增加植物生产力的方法
公开(公告)号：WO2012158296A1
公开(公告)日：2012-11-22
申请号：PCT/US2012/033916
申请日：2012-04-17
申请人： PLANT SENSORY SYSTEMS, LLC , TURANO, Frank J. , TURANO, Kathleen A.
发明人： TURANO, Frank J. , TURANO, Kathleen A.
IPC分类号： C12N15/82 , C12N9/88
CPC分类号： C12N15/8241 , A01H5/10 , C12N9/88 , C12N15/8261 , C12Y401/01029 , Y02A40/146
摘要： The present invention describes an approach to increase plant growth and production. The invention describes methods for the use of functional sulfinoalanine decarboxylase (SAD) or the promiscuous enzyme activity of SAD in plants or algal cells. Transgenic plants will have increased plant growth, biomass, yield, and/or tolerance to biotic and/or abiotic stresses and could be used as a pharmaceutical, nutraceutical or as a supplement in animal feed.
摘要翻译：本发明描述了增加植物生长和生产的方法。本发明描述了在植物或藻类细胞中使用功能性亚硫酰丙氨酸脱羧酶（SAD）或SAD的混杂酶活性的方法。转基因植物将具有增加的植物生长，生物量，产量和/或对生物和/或非生物胁迫的耐受性，并且可以用作动物饲料中的药物，营养保健品或补充剂。

2. 发明申请

WO2009032755A4 ALTERNATIVE METHODS FOR THE BIOSYNTHESIS OF GABA 审中-公开
标题翻译： GABA生物合成的替代方法
公开(公告)号：WO2009032755A4
公开(公告)日：2009-06-18
申请号：PCT/US2008074633
申请日：2008-08-28
申请人： PLANT SENSORY SYSTEM LLC , TURANO FRANK J , TURANO KATHLEEN A
发明人： TURANO FRANK J , TURANO KATHLEEN A
IPC分类号： C12P13/00 , C12P1/00
CPC分类号： C12N15/8243 , C12N9/0004 , C12N9/1096
摘要： The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.
摘要翻译：本发明描述了增加原核生物或真核生物中的GABA生产的替代方法，即通过在途径不存在或未明确鉴定的生物体中插入腐胺分解代谢途径。本发明描述了在植物中使用编码功能性腐胺氨基转移酶（PAT）和γ-氨基丁醛脱氢酶（GABAlde DeHase）多肽的多核苷酸以增加GABA产生的方法。本发明的优选实施方案在植物中，但可以使用其他生物体。 GABA可用性的变化将改善生长和增加对生物和非生物胁迫的耐受性。

3. 发明申请

WO2009032755A3 ALTERNATIVE METHODS FOR THE BIOSYNTHESIS OF GABA 审中-公开
标题翻译： GABA生物合成的替代方法
公开(公告)号：WO2009032755A3
公开(公告)日：2009-05-07
申请号：PCT/US2008074633
申请日：2008-08-28
申请人： PLANT SENSORY SYSTEM LLC , TURANO FRANK J , TURANO KATHLEEN A
发明人： TURANO FRANK J , TURANO KATHLEEN A
IPC分类号： C12P13/00 , C12P1/00
CPC分类号： C12N15/8243 , C12N9/0004 , C12N9/1096
摘要： The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.
摘要翻译：本发明描述了增加原核生物或真核生物中GABA生产的替代方法，即通过将腐胺分解代谢途径插入其中途径不存在或未明确鉴定的生物体中。本发明描述了在植物中使用编码功能性腐胺转氨酶（PAT）和γ-氨基丁醛脱氢酶（GABAlde脱氢酶）多肽的多核苷酸以增加GABA产生的方法。本发明的优选实施方案在植物中，但也可以使用其他生物体。 GABA可用性的变化将改善生长并提高对生物和非生物胁迫的耐受性。

4. 发明申请

WO2011053764A8 METHODS FOR THE BIOSYNTHESIS OF TAURINE OR HYPOTAURINE IN CELLS 审中-公开
标题翻译：牛磺酸或牛磺酸在细胞中的生物合成方法
公开(公告)号：WO2011053764A8
公开(公告)日：2011-07-28
申请号：PCT/US2010054664
申请日：2010-10-29
申请人： PLANT SENSORY SYSTEMS LLC , TURANO FRANK J , TURANO KATHLEEN A , CARLSON PETER S , KINNERSLEY ALAN M
发明人： TURANO FRANK J , TURANO KATHLEEN A , CARLSON PETER S , KINNERSLEY ALAN M
IPC分类号： C12N15/82
CPC分类号： A61K31/145 , A23K10/12 , A23K20/10 , A23L33/10 , A23V2002/00 , A61K31/185 , C12N9/0069 , C12N15/8243 , C12N15/8253 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P13/001 , C12Y113/1102 , Y02A40/146
摘要： The present invention describes an approach to increase taurine or hypotaurine production in prokaryotes or eukaryotes. More particularly, the invention relates to genetic transformation of organisms with genes that encode proteins that catalyze the conversion of cysteine to taurine, methionine to taurine, cysteamine to taurine, or alanine to taurine. The invention describes methods for the use of polynucleotides that encode functional cysteine dioxygenase (CDO), cysteine dioxygenase (CDO) and sulfinoalanine decarboxylase (SAD) or glutamate decarboxylase (GAD), cysteamine dioxygenase (ADO), taurine-pyruvate aminotransferase (TPAT), TPAT and sulfoacetaldehyde acetyltransferase (SA), taurine dioxygenase (TDO) or the small (ssTDeHase) and large subunits of taurine dehydrogenase (lsTDeHase) polypeptides in plants to increase taurine, hypotaurine or taurine precursor production. The preferred embodiment of the invention is in plants but other organisms may be used. Increased taurine production in plants will enhance plant growth and development, yield, or tolerance to biotic and/or abiotic stresses and could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed.
摘要翻译：本发明描述了增加原核生物或真核生物中牛磺酸或亚牛磺酸生产的方法。更具体地说，本发明涉及具有编码蛋白质的基因的生物体的遗传转化，所述蛋白质催化半胱氨酸转化为牛磺酸，甲硫氨酸转化为牛磺酸，半胱胺转化为牛磺酸或丙氨酸转化为牛磺酸。本发明描述了使用编码功能性半胱氨酸双加氧酶（CDO），半胱氨酸双加氧酶（CDO）和巯基丙氨酸脱羧酶（SAD）或谷氨酸脱羧酶（GAD），半胱胺双加氧酶（ADO），牛磺酸 - 丙酮酸氨基转移酶（TPAT） TPAT和磺基乙醛乙酰转移酶（SA），牛磺酸双加氧酶（TDO）或牛磺酸脱氢酶（lsTDeHase）多肽的小亚基（ssTDeHase）以增加牛磺酸，亚牛磺酸或牛磺酸前体的产生。本发明的优选实施方案在植物中，但也可以使用其他生物体。植物中牛磺酸生产的增加将增强植物生长和发育，产量或对生物和/或非生物胁迫的耐受性，并可用作营养品，药物或治疗化合物或作为动物饲料的补充剂。

5. 发明申请

WO2009029707A1 METABOLIC REGULATORS 审中-公开
标题翻译：代谢调节剂
公开(公告)号：WO2009029707A1
公开(公告)日：2009-03-05
申请号：PCT/US2008/074597
申请日：2008-08-28
申请人： PLANT SENSORY SYSTEM, LLC. , TURANO, Frank J. , TURANO, Kathleen A.
发明人： TURANO, Frank J. , TURANO, Kathleen A.
IPC分类号： C07K1/00
CPC分类号： C12N15/62 , C07K2319/00 , C12N15/8257
摘要： The present invention provides methods to make soluble or membrane-anchored metabolic regulators for use in prokaryotes or eukaryotes. The present invention provides methods of expressing novel protein constructs that bind to specific metabolites to control their availability in the cell. The invention utilizes bacterial periplasmic binding proteins, or domains from prokaryotic and eukaryotic proteins that are functionally similar to the bacterial periplasmic binding proteins, fused together and/or fused to a peptide that encodes a transmembrane domain. Changes in metabolite availability will result in altered metabolism or receptor activity to improve growth, yield, crop quality, or tolerance to biotic and abiotic stress.
摘要翻译：本发明提供了制备用于原核生物或真核生物的可溶性或膜锚定代谢调节剂的方法。本发明提供了表达与特异性代谢物结合以控制其在细胞中的可用性的新型蛋白质构建体的方法。本发明利用与编码跨膜结构域的肽融合在一起和/或融合的细菌周质结合蛋白的功能上类似的原核和真核蛋白质的细菌周质结合蛋白或结构域。代谢物可用性的变化将导致代谢或受体活性的改变，以改善生长，产量，作物质量或对生物和非生物胁迫的耐受性。

6. 发明申请

WO2009015096A2 GLUTAMATE RECEPTOR ASSOCIATED GENES AND PROTEINS FOR ENHANCING NITROGEN UTILIZATION EFFICIENCY IN CROP PLANTS 审中-公开
标题翻译：谷氨酸受体相关基因和蛋白质，用于增加作物种植中的氮素利用效率
公开(公告)号：WO2009015096A2
公开(公告)日：2009-01-29
申请号：PCT/US2008/070663
申请日：2008-07-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , THE GEORGE WASHINGTON UNIVERSITY , HERSHEY, Howard, P. , FRANK, Mary, J. , SIMMONS, Carl, R. , TURANO, Frank, J.
发明人： HERSHEY, Howard, P. , FRANK, Mary, J. , SIMMONS, Carl, R. , TURANO, Frank, J.
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： Abstract The invention provides isolated glutamate receptor associated nucleic acids and their encoded proteins for modulating nitrogen utilization efficiency in plants. The invention includes methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
摘要翻译：摘要本发明提供了分离的谷氨酸受体相关核酸及其编码的蛋白质，用于调节植物的氮利用效率。本发明包括与改变植物中氮利用和/或摄取有关的方法和组合物。本发明还提供了重组表达盒，宿主细胞和转基因植物。

7. 发明申请

WO2012151071A3 REGULATORY SEQUENCES TO CONTROL GENE EXPRESSION IN PLANTS 审中-公开
标题翻译：控制植物基因表达的调节序列
公开(公告)号：WO2012151071A3
公开(公告)日：2014-05-30
申请号：PCT/US2012034661
申请日：2012-04-23
申请人： PLANT SENSORY SYSTEMS LLC , TURANO FRANK J , TURANO KATHLEEN A
发明人： TURANO FRANK J , TURANO KATHLEEN A
IPC分类号： C07H21/04 , C12N15/82
CPC分类号： C12N15/8216 , C12N15/8223 , C12N15/8225
摘要： The present invention discloses regulatory sequences, promoters and terminators, and their use in plants. The regulatory sequences can be used to make gene constructs that include a gene not natively associated with the regulatory sequences. Methods to use the regulatory sequences with antisense constructs or functional RNAs are disclosed. Methods to use the regulatory sequences, promoter or terminator, independently of each other are also disclosed. Methods to use the regulatory sequences to improve plant growth and production such as increased biomass, increased yield and increased tolerance to abiotic or biotic stresses are also disclosed.
摘要翻译：本发明公开了调节序列，启动子和终止子及其在植物中的用途。调节序列可用于制备基因构建体，其包括与调节序列本身无关联的基因。公开了使用具有反义构建体或功能性RNA的调节序列的方法。还公开了彼此独立地使用调节序列，启动子或终止子的方法。还公开了使用调节序列改善植物生长和生产的方法，例如增加的生物量，增加的产量和增加的对非生物或生物胁迫的耐受性。

8. 发明申请

WO2012151071A2 REGULATORY SEQUENCES TO CONTROL GENE EXPRESSION IN PLANTS 审中-公开
标题翻译：控制植物基因表达的调节序列
公开(公告)号：WO2012151071A2
公开(公告)日：2012-11-08
申请号：PCT/US2012/034661
申请日：2012-04-23
申请人： PLANT SENSORY SYSTEMS, LLC , TURANO, Frank J. , TURANO, Kathleen A.
发明人： TURANO, Frank J. , TURANO, Kathleen A.
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8216 , C12N15/8223 , C12N15/8225
摘要： The present invention discloses regulatory sequences, promoters and terminators, and their use in plants. The regulatory sequences can be used to make gene constructs that include a gene not natively associated with the regulatory sequences. Methods to use the regulatory sequences with antisense constructs or functional RNAs are disclosed. Methods to use the regulatory sequences, promoter or terminator, independently of each other are also disclosed. Methods to use the regulatory sequences to improve plant growth and production such as increased biomass, increased yield and increased tolerance to abiotic or biotic stresses are also disclosed.
摘要翻译：本发明公开了调节序列，启动子和终止子及其在植物中的用途。调节序列可用于制备基因构建体，其包括与调节序列本身无关联的基因。公开了使用具有反义构建体或功能性RNA的调节序列的方法。还公开了彼此独立地使用调节序列，启动子或终止子的方法。还公开了使用调节序列改善植物生长和生产的方法，例如增加的生物量，增加的产量和增加的对非生物或生物胁迫的耐受性。

9. 发明申请

WO2003007886A3 PLANT ION CHANNELS AND METHODS 审中-公开
公开(公告)号：WO2003007886A3
公开(公告)日：2003-01-30
申请号：PCT/US2002/023180
申请日：2002-07-19
申请人： EMERALD BIOAGRICULTURE CORPORATION , KINNERSLEY, Alan, M. , TURANO, Frank, J.
发明人： KINNERSLEY, Alan, M. , TURANO, Frank, J.
IPC分类号： C12N15/63
摘要： Recombinant plant receptor proteins are provided, as are nucleotide sequences encoding these proteins. The invention also provides recombinant vectors including the nucleotide sequences encoding the proteins. Further provided are plant host cells that include the recombinant vectors, transgenic plants and methods of using the nucleotide and amino acid sequences described herein, including methods of treating plants, methods of expressing the proteins described herein, methods of modifying receptor activity in a plant and methods of regulating plant metabolism. Inventive plant receptor proteins are expected to function as effective modulators of the effects of GABA.

10. 发明申请

WO2011053764A3 METHODS FOR THE BIOSYNTHESIS OF TAURINE OR HYPOTAURINE IN CELLS 审中-公开
公开(公告)号：WO2011053764A3
公开(公告)日：2011-05-05
申请号：PCT/US2010/054664
申请日：2010-10-29
申请人： PLANT SENSORY SYSTEMS, LLC , TURANO, Frank, J. , TURANO, Kathleen, A. , CARLSON, Peter, S. , KINNERSLEY, Alan, M.
发明人： TURANO, Frank, J. , TURANO, Kathleen, A. , CARLSON, Peter, S. , KINNERSLEY, Alan, M.
IPC分类号： C12N15/82 , C07C309/14 , A23L1/00 , A61K31/00 , A61K31/185 , A23L1/305
摘要： The present invention describes an approach to increase taurine or hypotaurine production in prokaryotes or eukaryotes. More particularly, the invention relates to genetic transformation of organisms with genes that encode proteins that catalyze the conversion of cysteine to taurine, methionine to taurine, cysteamine to taurine, or alanine to taurine. The invention describes methods for the use of polynucleotides that encode functional cysteine dioxygenase (CDO), cysteine dioxygenase (CDO) and sulfinoalanine decarboxylase (SAD) or glutamate decarboxylase (GAD), cysteamine dioxygenase (ADO), taurine-pyruvate aminotransferase (TPAT), TPAT and sulfoacetaldehyde acetyltransferase (SA), taurine dioxygenase (TDO) or the small (ssTDeHase) and large subunits of taurine dehydrogenase (lsTDeHase) polypeptides in plants to increase taurine, hypotaurine or taurine precursor production. The preferred embodiment of the invention is in plants but other organisms may be used. Increased taurine production in plants will enhance plant growth and development, yield, or tolerance to biotic and/or abiotic stresses and could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed.

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