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1. 发明申请

WO2010058418A3 NOVEL SYNTHETIC EXPRESSION VEHICLE 审中-公开
标题翻译：新型合成表情车
公开(公告)号：WO2010058418A3
公开(公告)日：2010-09-30
申请号：PCT/IN2009000610
申请日：2009-10-28
申请人： USV LTD , RAO LAXMI SRINIVAS , MISHRA SHRIKANT , NIPHADKAR MILIND , THAKUR PRITI , NAZARETH GENEVIEVE
发明人： RAO LAXMI SRINIVAS , MISHRA SHRIKANT , NIPHADKAR MILIND , THAKUR PRITI , NAZARETH GENEVIEVE
IPC分类号： C12N15/70 , C12R1/19
CPC分类号： C12N15/70
摘要： An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E.coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between -55 to -10 and ribosome binding site from the transcription initiation site.
摘要翻译：包含如Seq ID No.1所示的分离的核酸的表达载体，其包含合成的杂合启动子，其中所述杂合启动子包含来源于大肠杆菌的诱导型阿拉伯糖启动子和来自pHO调节子的8-35个核苷酸的合成片段在-55至-10之间的区域和与转录起始位点的核糖体结合位点。

2. 发明申请

WO2010058418A2 NOVEL SYNTHETIC EXPRESSION VEHICLE 审中-公开
标题翻译：新型合成表达车
公开(公告)号：WO2010058418A2
公开(公告)日：2010-05-27
申请号：PCT/IN2009/000610
申请日：2009-10-28
申请人： USV LIMITED , RAO, Laxmi Srinivas , MISHRA, Shrikant , NIPHADKAR, Milind , THAKUR, Priti , NAZARETH, Genevieve
发明人： RAO, Laxmi Srinivas , MISHRA, Shrikant , NIPHADKAR, Milind , THAKUR, Priti , NAZARETH, Genevieve
IPC分类号： C12N15/09
CPC分类号： C12N15/70
摘要： An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E.coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between -55 to -10 and ribosome binding site from the transcription initiation site.
摘要翻译：包含如Seq ID No.1所示的分离的核酸的表达载体，其包含合成的杂合启动子，其中杂合启动子包含源自大肠杆菌的诱导型阿拉伯糖启动子和合成的在-55至-10之间的区域引入来自pHO调节子的8-35个核苷酸和来自转录起始位点的核糖体结合位点。

3. 发明申请

WO2009005973A3 SYNTHETIC GENE FOR ENHANCED EXPRESSION IN E.COLI 审中-公开
标题翻译：在E.COLI中增强表达的合成基因
公开(公告)号：WO2009005973A3
公开(公告)日：2009-12-30
申请号：PCT/US2008066496
申请日：2008-06-11
申请人： PHARMACEUTICAL PATENT ATTORNEY , RAO LAXMI SRINIVAS , AHMED MONSUR BORBHUIYA , NIPHADKAR MILIND , PALIWAL DINESH , SHEKHAWAT RAKESH , KHARE ARUNA , SAMANT RADHIKA , THAKUR PRITI , CHUTKE ANJALI , SURLIKAR NILESH , ZAWAR SAGAR , MISHRA SHRIKANT , USV LTD
发明人： RAO LAXMI SRINIVAS , AHMED MONSUR BORBHUIYA , NIPHADKAR MILIND , PALIWAL DINESH , SHEKHAWAT RAKESH , KHARE ARUNA , SAMANT RADHIKA , THAKUR PRITI , CHUTKE ANJALI , SURLIKAR NILESH , ZAWAR SAGAR , MISHRA SHRIKANT
IPC分类号： C12P21/04
CPC分类号： C12P21/02 , C07K14/58
摘要： A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for E.coli and has optimal RNA stability. The inventors also provide a process for large scale purification of nesiritide by pH precipitation and chromatography.
摘要翻译：编码人b型利尿钠肽（hBNP）或奈西立肽的新型奈西立肽合成cDNA嵌合体以及制备所述新嵌合体的方法。此外，本发明人公开了使用奈西立肽合成cDNA嵌合体获得可表达的构建体以产生成熟的奈西立肽。具体而言，本发明人公开了应用重组克隆方法来制备奈西立肽嵌合构建体的ORF，其对大肠杆菌同时密码子优化并且具有最佳的RNA稳定性。本发明人还提供了通过pH沉淀和色谱法大规模纯化奈西立肽的方法。

4. 发明申请

WO2007096899A3 AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：用于纯化重组蛋白的亲和聚合物（AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS）
公开(公告)号：WO2007096899A3
公开(公告)日：2008-03-13
申请号：PCT/IN2007000051
申请日：2007-02-07
申请人： USV LTD , RAO LAXMI S , MISHRA SHRIKANT , BORBHUIYA MONSUR AHMED , MHATRE DEEPA , THAKUR PRITI
发明人： RAO LAXMI S , MISHRA SHRIKANT , BORBHUIYA MONSUR AHMED , MHATRE DEEPA , THAKUR PRITI
IPC分类号： C12N15/62
CPC分类号： C12N15/62
摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.
摘要翻译：本公开提供了用于纯化重组生物活性蛋白或多肽的亲和多肽。此外，本公开提供了融合重组蛋白或多肽，其中融合重组蛋白包含至少两种组分，生物活性多肽或感兴趣的蛋白质或亲和性多肽。生物活性多肽可以通过共价结合直接或间接地连接到亲和多肽上。本公开内容提供了用于在宿主细胞中产生所述融合重组蛋白的重组表达载体。此外，本公开提供了从宿主细胞纯化重组蛋白质的改进方法。此外，本公开提供了通过固定金属离子螯合色谱法纯化重组生物活性多肽或蛋白质的方法。

5. 发明申请

WO2009019715A1 NOVEL ORTHOGONAL PROCESS FOR PURIFICATION OF RECOMBINANT HUMAN PARATHYROID HORMONE (RHPTH) (1-34) 审中-公开
标题翻译：用于纯化重组人类PARAMYROID HORMONE（RHPTH）的新型正交方法（1-34）
公开(公告)号：WO2009019715A1
公开(公告)日：2009-02-12
申请号：PCT/IN2007/000573
申请日：2007-12-06
申请人： USV LIMITED , SANKARARAMAN, Uma , MAITI, Dipanwita , SANKARANKUTTY, Meera , SHEKHAWAT, Rakesh , KOPPARAPU, Narasimha, Kumar , ZAIDI, Gulnaz , RATHOD, Bipinchandra , THAKAR, Niren, Praful , THAKUR, Priti , CHUTKE, Anjali , MISHRA, Shrikant
发明人： SANKARARAMAN, Uma , MAITI, Dipanwita , SANKARANKUTTY, Meera , SHEKHAWAT, Rakesh , KOPPARAPU, Narasimha, Kumar , ZAIDI, Gulnaz , RATHOD, Bipinchandra , THAKAR, Niren, Praful , THAKUR, Priti , CHUTKE, Anjali , MISHRA, Shrikant
IPC分类号： C07K14/635 , C07K1/36
CPC分类号： C07K14/635 , C07K2319/21 , C07K2319/35 , C07K2319/50 , C07K2319/61
摘要： The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragemt as set forth in SEQ. ID. No.:l encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partrner consisting of 41 amino acids belonging to Escherichia coli β-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transforming Escherichia coli with the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in Escherichia coli. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP- HPLC to yield a target protein of ≥ 99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).
摘要翻译：本发明公开了一种制备rhPTH（1-34）的方法，其也称为特立帕肽，通过构建新的核苷酸，如SEQ ID NO：1所示的NcoI.IXhoI fragemt。 ID。编号：编码如SEQ.ID所示的嵌合融合蛋白。编号：2包含由属于大肠杆菌β-半乳糖苷酶（LacZ）基因的41个氨基酸，内肽酶切割位点，rhPTH（1-34）基因片段组成的融合成分器，将所述核苷酸克隆在表达载体中控制T7启动子，用所述载体转化大肠杆菌并在补料分批发酵中表达嵌合融合蛋白。本发明还公开了用于在大肠杆菌中优化rhPTH（1-34）表达的低进料速率乳糖诱导。本发明还公开了一种用于rhPTH（1-34）的独特的新型两步正交纯化方法，其包括阳离子交换层析，任选地随后进行选自HIC或RP-HPLC的制备型色谱，得到纯度为99％的目标蛋白质。本发明公开了一种生产高纯度rhPTH（1-34）的简单，成本效益高，环境友好的方法。

6. 发明申请

WO2009005973A2 SYNTHETIC GENE FOR ENHANCED EXPRESSION IN E.COLI 审中-公开
标题翻译：合成基因在E.COLI中的增强表达
公开(公告)号：WO2009005973A2
公开(公告)日：2009-01-08
申请号：PCT/US2008/066496
申请日：2008-06-11
申请人： PHARMACEUTICAL PATENT ATTORNEYS, LLC , USV LTD. , RAO, Laxmi, Srinivas , AHMED, Monsur, Borbhuiya , NIPHADKAR, Milind , PALIWAL, Dinesh , SHEKHAWAT, Rakesh , KHARE, Aruna , SAMANT, Radhika , THAKUR, Priti , CHUTKE, Anjali , SURLIKAR, Nilesh , ZAWAR, Sagar , MISHRA, Shrikant
发明人： RAO, Laxmi, Srinivas , AHMED, Monsur, Borbhuiya , NIPHADKAR, Milind , PALIWAL, Dinesh , SHEKHAWAT, Rakesh , KHARE, Aruna , SAMANT, Radhika , THAKUR, Priti , CHUTKE, Anjali , SURLIKAR, Nilesh , ZAWAR, Sagar , MISHRA, Shrikant
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K14/58
摘要： A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for E.coli and has optimal RNA stability. The inventors also provide a process for large scale purification of nesiritide by pH precipitation and chromatography.
摘要翻译：编码人b型利尿钠肽（hBNP）或奈西立肽的新型奈西立肽合成cDNA嵌合体及其制备方法。此外，发明人公开了使用奈西立肽合成cDNA嵌合体以获得可表达的构建体以产生成熟的奈西立肽。特别地，本发明人公开了重组克隆方法的应用，以制备奈西立肽嵌合构建体的ORF，该嵌合构建体对于大肠杆菌同时密码子优化并具有最佳的RNA稳定性。本发明人还提供了通过pH沉淀和色谱法大规模纯化奈西立胺的方法。

7. 发明申请

WO2007096899A2 AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：用于纯化重组蛋白的亲和聚合物（AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS）
公开(公告)号：WO2007096899A2
公开(公告)日：2007-08-30
申请号：PCT/IN2007/000051
申请日：2007-02-07
申请人： USV LIMITED , RAO, Laxmi, S. , MISHRA, Shrikant , BORBHUIYA, Monsur, Ahmed , MHATRE, Deepa , THAKUR, Priti
发明人： RAO, Laxmi, S. , MISHRA, Shrikant , BORBHUIYA, Monsur, Ahmed , MHATRE, Deepa , THAKUR, Priti
IPC分类号： C07K14/43
CPC分类号： C12N15/62
摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.
摘要翻译：本公开提供了用于纯化重组生物活性蛋白或多肽的亲和多肽。此外，本公开提供了融合重组蛋白或多肽，其中融合重组蛋白包含至少两种组分，生物活性多肽或感兴趣的蛋白质或亲和性多肽。生物活性多肽可以通过共价结合直接或间接地连接到亲和多肽上。本公开内容提供了用于在宿主细胞中产生所述融合重组蛋白的重组表达载体。此外，本公开提供了从宿主细胞纯化重组蛋白质的改进方法。此外，本公开提供了通过固定金属离子螯合色谱法纯化重组生物活性多肽或蛋白质的方法。

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