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1. 发明申请

WO2009133025A1 CONTROLLED CDNA OVEREXPRESSION SYSTEM IN ARABIDOPSIS 审中-公开
标题翻译：控制CDNA过度表达系统在阿拉伯语
公开(公告)号：WO2009133025A1
公开(公告)日：2009-11-05
申请号：PCT/EP2009/054946
申请日：2009-04-24
申请人： BIOLOGICAL RESEARCH CENTER OF THE HUNGARIAN ACADEMY OF SCIENCES , SZABADOS, László , KONCZ, Csaba , TEMESVÁRINÉ ÁBRAHÁM, Edit , PAPDI, Csaba , PRATHYBA JOSEPH, Mary
发明人： SZABADOS, László , KONCZ, Csaba
IPC分类号： C12N15/82
CPC分类号： C12N15/8237 , C12N15/8271 , C12N15/8273
摘要： The present invention provides a method for identifying genes being responsible for stress regulatory functions in Arabidopsis, comprising cloning an Arabidopsis cDNA library into a vector suitable for inducible expression of the introduced nucleic acid in a position allowing the expression of said nucleic acid, introducing the cloned cDNA library into wild-type Arabidopsis; regenerating plants comprising said expression vector carrying a cDNA insert as being produced; testing the plants regenerated for stress tolerance upon inducing said vector to express the cDNA insert, identifying plants with modified stress tolerance, and identifying the gene(s) being comprised in the cDNA insert of said expression vector produced in step a) being present in said stress tolerant plant as gene(s) capable of modifying the stress tolerance in Arabidopsis. The invention further provides novel genes involved in the stress regulatory functions identified by the present method, the uses thereof for enhancing the stress tolerance of plants, as well es kits to perform the method of the invention.
摘要翻译：本发明提供了一种用于鉴定在拟南芥中负责应激调节功能的基因的方法，包括将拟南芥cDNA文库克隆到适于诱导性表达引入的核酸的载体中，所述载体允许表达所述核酸，引入克隆 cDNA文库进入野生型拟南芥; 再生植物，其包含携带产生的cDNA插入物的所述表达载体; 在诱导所述载体表达cDNA插入物，鉴定具有修饰的胁迫耐受性的植物并鉴定包含在所述表达载体的cDNA插入物中的基因时，测试植物被再生以产生胁迫耐受性，所述基因存在于所述胁迫耐受植物作为能够修饰拟南芥胁迫耐受性的基因。本发明还提供涉及通过本方法鉴定的应激调节功能的新基因，其用于增强植物的胁迫耐受性的用途，以及用于实施本发明方法的试剂盒。

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