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1. 发明申请

WO2004068104A3 TARGET ACTIVATED MICROTRANSFER 审中-公开
公开(公告)号：WO2004068104A3
公开(公告)日：2004-08-12
申请号：PCT/US2003/023317
申请日：2003-07-23
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH , EMMERT-BUCK, Michael, R. , TANGREA, Michael, A. , BONNER, Robert, F. , CHUAQUI, Rodrigo, F.
发明人： EMMERT-BUCK, Michael, R. , TANGREA, Michael, A. , BONNER, Robert, F. , CHUAQUI, Rodrigo, F.
IPC分类号： G01N33/543
摘要： A method of removing a target from a biological sample which involved placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere or to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly ( for example with a heat generating enzyme carried by the immunoreagent), or indirectly ( for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorosphore that absorbs light and emits heat.

2. 发明申请

WO2010088517A1 METHODS AND SYSTEMS FOR PURIFYING TRANSFERRING AND/OR MANIPULATING NUCLEIC ACIDS 审中-公开
标题翻译：用于净化转运和/或操作核酸的方法和系统
公开(公告)号：WO2010088517A1
公开(公告)日：2010-08-05
申请号：PCT/US2010/022586
申请日：2010-01-29
申请人： THE U.S.A., AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES , UNIVERSITY OF MARYLAND, COLLEGE PARK , EMMERT-BUCK, Michael, R. , ARMANI, Michael , SMELA, Elisabeth , SHAPIRO, Benjamin , TANGREA, Michael, A. , RODRIGUEZ-CANALES, Jaime , CHUAQUI, Rodrigo , GILLESPIE, John
发明人： EMMERT-BUCK, Michael, R. , ARMANI, Michael , SMELA, Elisabeth , SHAPIRO, Benjamin , TANGREA, Michael, A. , RODRIGUEZ-CANALES, Jaime , CHUAQUI, Rodrigo , GILLESPIE, John
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , B01L3/5025 , B01L3/50853 , B01L3/50857 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0487 , C12N15/1006 , C12Q2565/631 , C12Q2565/513 , C12Q2547/101 , C12Q2523/125
摘要： The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information.
摘要翻译：本公开提供用于纯化，转移或操纵核酸的方法，系统和装置，同时保持核酸的2D空间关系，因为它们存在于具有2D空间信息的原始样品中。

3. 发明申请

WO2004048928A2 METHOD AND APPARATUS FOR PERFORMING MULTIPLE SIMULTANEOUS MANIPULATIONS OF BIOMOLECULES IN A TWO-DIMENSIONAL ARRAY 审中-公开
标题翻译：用于在二维阵列中执行生物分子的多次同时操作的方法和装置
公开(公告)号：WO2004048928A2
公开(公告)日：2004-06-10
申请号：PCT/US2003/037208
申请日：2003-11-20
申请人： THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF HEALTH AND HUMAN SERVICES, NIH , EMMERT-BUCK, Michael, R. , CHUAQUI, Rodrigo, F. , TANGREA, Michael, A.
发明人： EMMERT-BUCK, Michael, R. , CHUAQUI, Rodrigo, F. , TANGREA, Michael, A.
IPC分类号： G01N
CPC分类号： C12Q1/6837 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , C12Q1/6806
摘要： This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.
摘要翻译：本发明涉及在二维阵列（例如凝胶，膜，组织活组织检查等）中进行多重同时操作生物分子的方法和装置。这种操作特别包括测定和核酸扩增方案。

4. 发明申请

WO2010088517A8 METHODS AND SYSTEMS FOR PURIFYING TRANSFERRING AND/OR MANIPULATING NUCLEIC ACIDS 审中-公开
标题翻译：用于净化转运和/或操作核酸的方法和系统
公开(公告)号：WO2010088517A8
公开(公告)日：2011-07-28
申请号：PCT/US2010022586
申请日：2010-01-29
申请人： US HEALTH , UNIV MARYLAND , EMMERT-BUCK MICHAEL R , ARMANI MICHAEL , SMELA ELISABETH , SHAPIRO BENJAMIN , TANGREA MICHAEL A , RODRIGUEZ-CANALES JAIME , CHUAQUI RODRIGO , GILLESPIE JOHN
发明人： EMMERT-BUCK MICHAEL R , ARMANI MICHAEL , SMELA ELISABETH , SHAPIRO BENJAMIN , TANGREA MICHAEL A , RODRIGUEZ-CANALES JAIME , CHUAQUI RODRIGO , GILLESPIE JOHN
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , B01L3/5025 , B01L3/50853 , B01L3/50857 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0487 , C12N15/1006 , C12Q2565/631 , C12Q2565/513 , C12Q2547/101 , C12Q2523/125
摘要： The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information.
摘要翻译：本公开提供用于纯化，转移或操纵核酸的方法，系统和装置，同时保持核酸的2D空间关系，因为它们存在于具有2D空间信息的原始样品中。

5. 发明申请

WO2004048928A3 METHOD AND APPARATUS FOR PERFORMING MULTIPLE SIMULTANEOUS MANIPULATIONS OF BIOMOLECULES IN A TWO-DIMENSIONAL ARRAY 审中-公开
标题翻译：用于在二维阵列中执行生物分子的多个同时操纵的方法和设备
公开(公告)号：WO2004048928A3
公开(公告)日：2004-09-16
申请号：PCT/US0337208
申请日：2003-11-20
申请人： US HEALTH , EMMERT-BUCK MICHAEL R , CHUAQUI RODRIGO F , TANGREA MICHAEL A
发明人： EMMERT-BUCK MICHAEL R , CHUAQUI RODRIGO F , TANGREA MICHAEL A
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , C12Q1/6806
摘要： This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.
摘要翻译：本发明涉及用于以二维阵列（诸如凝胶，膜，组织活检等）对生物分子进行多个同时操作的方法和装置，这种操作特别包括测定和核酸扩增方案。

6. 发明申请

WO2004068104A2 TARGET ACTIVATED MICROTRANSFER 审中-公开
标题翻译：靶向活性微转运体
公开(公告)号：WO2004068104A2
公开(公告)日：2004-08-12
申请号：PCT/US0323317
申请日：2003-07-23
申请人： US GOV HEALTH & HUMAN SERV , EMMERT-BUCK MICHAEL R , TANGREA MICHAEL A , BONNER ROBERT F , CHUAQUI RODRIGO F
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL A , BONNER ROBERT F , CHUAQUI RODRIGO F
IPC分类号： G01N33/543 , G01N33/569 , G01N
CPC分类号： G01N33/56966 , G01N33/543 , Y10T428/2813 , Y10T428/2839
摘要： A method of removing a target from a biological sample which involved placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere or to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly ( for example with a heat generating enzyme carried by the immunoreagent), or indirectly ( for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorosphore that absorbs light and emits heat.
摘要翻译：一种从生物样品中去除目标物的方法，其涉及将转印表面与生物样品接触，然后局部地改变转印表面以允许目标物与生物样品的选择性分离。在公开的实施例中，目标是组织切片的细胞或细胞组分，并且转印表面是可以局部改变以将目标粘附到转印表面的膜。随后从组织部分分离膜选择性地从组织部分移除粘附的目标。例如通过加热靶以粘附或热塑性转印表面，转印表面从靶内激活以将靶附着到转印表面。这种原位激活可通过将生物样品暴露于特异性结合靶标（或靶标组分）的免疫试剂来实现。免疫试剂可以直接改变转移表面（例如用免疫试剂携带的生热酶），或间接改变（例如通过改变靶标的特征）。在一些实施方案中，免疫试剂将沉淀物沉积在靶中，相对于周围组织增加其光吸收，使得生物样本可暴露于光以选择性地加热靶。或者，免疫试剂是携带吸收光并发热的荧光磷光体的免疫荧光剂。

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