专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2010147673A3 SINGLE-DNA MOLECULE NANOMOTOR REGULATED BY PHOTONS 审中-公开
标题翻译：单光子法测定单分子纳米分子
公开(公告)号：WO2010147673A3
公开(公告)日：2011-06-03
申请号：PCT/US2010001772
申请日：2010-06-18
申请人： UNIV FLORIDA , TAN WEIHONG , KANG HUAIZHI
发明人： TAN WEIHONG , KANG HUAIZHI
IPC分类号： C12N15/11 , C07H21/04 , C12Q1/68
CPC分类号： C12N15/11 , B82Y5/00 , C12N15/10
摘要： This invention provides photon-driven single-molecule nanomotors with biologically- based components, and methods of making the same. The nanomotors of the invention comprise a DNA hairpin-structured molecule incorporated with at least one azobenzene moiety (such as azobenzene phosphoramidite). The azobenzene moiety (or moieties) facilitates reversible photocontrollable conversion of the DNA molecule from the hairpin structure to an open state. This reversible extension-contraction behavior is considered a molecular motor function.
摘要翻译：本发明提供具有基于生物学的成分的光子驱动的单分子纳米电动机及其制备方法。本发明的纳米电动机包含与至少一个偶氮苯部分结合的DNA发夹结构分子（例如偶氮苯亚磷酰胺）。偶氮苯部分（或部分）促进DNA分子从发夹结构到开放状态的可逆可光控转化。这种可逆的拉伸收缩行为被认为是分子运动功能。

2. 发明申请

WO2010071852A3 OLIGONUCLEOTIDE MICELLES 审中-公开
标题翻译：寡核苷酸
公开(公告)号：WO2010071852A3
公开(公告)日：2010-12-16
申请号：PCT/US2009068864
申请日：2009-12-18
申请人： UNIV FLORIDA , TAN WEIHONG , HAIPENG LIU , WU YANRONG
发明人： TAN WEIHONG , HAIPENG LIU , WU YANRONG
IPC分类号： C12N15/113 , A61K31/7088 , A61P35/00 , C12N15/115
CPC分类号： A61K31/7088 , A61K9/0019 , A61K9/1075 , C12N15/111 , C12N2310/3515 , C12N2320/32
摘要： The present invention provides homogenous populations of micelles, methods for preparing these populations, methods for treating or preventing a disease or disorder using the population of micelles of the invention.
摘要翻译：本发明提供了均匀的胶束群，制备这些种群的方法，使用本发明的胶束群治疗或预防疾病或病症的方法。

3. 发明申请

WO2009035554A2 SUPERIOR STRUCTURE STABILITY AND SELECTIVITY OF HAIRPIN NUCLEIC ACID PROBES WITH AN L-DNA STEM 审中-公开
标题翻译：具有L-DNA STEM的头发核酸探针的优良结构稳定性和选择性
公开(公告)号：WO2009035554A2
公开(公告)日：2009-03-19
申请号：PCT/US2008/010466
申请日：2008-09-08
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , TAN, Weihong , KIM, Youngmi , YANG, Chaoyong, James
发明人： TAN, Weihong , KIM, Youngmi , YANG, Chaoyong, James
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , G01N33/58
CPC分类号： C12Q1/6832 , C12Q2525/301 , C12Q2525/101
摘要： Hairpin-shaped nucleic acid probes capable of forming stable hybrids with target nucleic acid sequences are provided. In particular, an oligonucleotide probe for hybridization analysis is provided, wherein the probe comprises a non-natural enantiomeric DNA (L-DNA) that forms the stem and natural DNA (D-DNA) that forms the loop in the hairpin. The invention thereby provides stable probes that experience fewer stem invasions and demonstrate sensitive and selective hybridization to target nucleic acid sequences. The subject probes are useful for a variety of biological and biotechnological applications.
摘要翻译：提供了能够与靶核酸序列形成稳定杂交体的发夹形核酸探针。具体而言，提供了用于杂交分析的寡核苷酸探针，其中探针包含形成茎的非天然对映体DNA（L-DNA）和形成发夹中环的天然DNA（D-DNA）。由此，本发明提供了经历较少茎侵入并证明对靶核酸序列敏感和选择性杂交的稳定探针。主题探针可用于各种生物和生物技术应用。

4. 发明申请

WO2007142713A2 METHODS FOR THE PRODUCTION OF HIGHLY SENSITIVE AND SPECIFIC CELL SURFACE PROBES 审中-公开
标题翻译：用于生产高敏感和特异性细胞表面探针的方法
公开(公告)号：WO2007142713A2
公开(公告)日：2007-12-13
申请号：PCT/US2007006054
申请日：2007-03-08
申请人： UNIV FLORIDA , TAN WEIHONG , SHANGGUAN DIHUA , LI YING
发明人： TAN WEIHONG , SHANGGUAN DIHUA , LI YING
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/3517
摘要： A system and method for producing an oligonucleotide having a high affinity for extracellular or cell surface markers on a target cell. The resultant oligonucleotide probe can be used to detect a target biomolecule, in particular a cancer cell or infectious agent such as a bacterium, virus, or fungus, comprising an aptamer having a high affinity for the biomolecule, wherein at least one labeled dye is attached to the aptamer. The labeled dye causes the aptamer to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target biomolecule, the fluorescence emission changes from the baseline emission to an emission that is visually detectable.
摘要翻译：用于制备对目标细胞上细胞外或细胞表面标记具有高亲和力的寡核苷酸的系统和方法。所得到的寡核苷酸探针可用于检测目标生物分子，特别是检测对生物分子具有高亲和力的适配体的细胞，病毒或真菌的癌细胞或感染因子，其中附着至少一种标记的染料适应者标记的染料导致适体发出基线，不可见的发射。当本发明的适体（本文也称为探针）与靶生物分子相互作用时，荧光发射从基线发射变化为视觉上可检测的发射。

5. 发明申请

WO2007075456A3 COLORIMETRIC LIGHT SWITCHING PROBE FOR ULTRASENSITIVE DETECTION OF TARGET COMPOUNDS 审中-公开
标题翻译：用于目标化合物超声检测的彩色光开关探针
公开(公告)号：WO2007075456A3
公开(公告)日：2007-11-01
申请号：PCT/US2006047988
申请日：2006-12-15
申请人： UNIV FLORIDA , UNIV COLUMBIA , TAN WEIHONG , YANG CHAOYONG , VICENS MARIE C , TURRO NICHOLAS J , JOCKUSCH STEFFEN
发明人： TAN WEIHONG , YANG CHAOYONG , VICENS MARIE C , TURRO NICHOLAS J , JOCKUSCH STEFFEN
IPC分类号： G01N33/533 , C12Q1/00 , C12Q1/68
CPC分类号： G01N33/5308 , G01N33/542 , G01N33/57488 , G01N2333/49
摘要： A system and method for detecting a target compound, comprising an aptamer having a high affinity for the target compound, wherein two labels are attached to the aptamer. The two labels cause the probe to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target compound, the probe undergoes a conformational change, causing the fluorescence emission to change from the baseline emission to an emission that is detectable.
摘要翻译：一种用于检测目标化合物的系统和方法，包括对靶化合物具有高亲和力的适体，其中两个标记附着于适体。两个标签使探针发出基线，不可见的发射。当本发明的适体（本文也称为探针）与目标化合物相互作用时，探针经历构象变化，导致荧光发射从基线发射改变为可检测的发射。

6. 发明申请

WO2007075456A2 COLORIMETRIC LIGHT SWITCHING PROBE FOR ULTRASENSITIVE DETECTION OF TARGET COMPOUNDS 审中-公开
标题翻译：比色光开关探针用于目标化合物的超灵敏检测
公开(公告)号：WO2007075456A2
公开(公告)日：2007-07-05
申请号：PCT/US2006/047988
申请日：2006-12-15
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , COLUMBIA UNIVERSITY , TAN, Weihong , YANG, Chaoyong , VICENS, Marie, C. , TURRO, Nicholas, J. , JOCKUSCH, Steffen
发明人： TAN, Weihong , YANG, Chaoyong , VICENS, Marie, C. , TURRO, Nicholas, J. , JOCKUSCH, Steffen
CPC分类号： G01N33/5308 , G01N33/542 , G01N33/57488 , G01N2333/49
摘要： A system and method for detecting a target compound, comprising an aptamer having a high affinity for the target compound, wherein two labels are attached to the aptamer. The two labels cause the probe to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target compound, the probe undergoes a conformational change, causing the fluorescence emission to change from the baseline emission to an emission that is detectable.
摘要翻译：用于检测目标化合物的系统和方法，包括对目标化合物具有高亲和力的适体，其中两个标记与适体连接。这两个标签会使探针发出基线不可见的发射。当本发明的适体（本文也称为探针）与目标化合物相互作用时，探针发生构象变化，引起荧光发射从基线发射变为可检测的发射。

7. 发明申请

WO2007044712A2 PORTABLE MATERIALS AND METHODS FOR ULTRASENSITIVE DETECTION OF PATHOGENS AND BIOPARTICLES 审中-公开
标题翻译：便携式材料和方法超灵敏检测病原体和生物微粒
公开(公告)号：WO2007044712A2
公开(公告)日：2007-04-19
申请号：PCT/US2006039536
申请日：2006-10-10
申请人： UNIV FLORIDA , TAN WEIHONG , MECHERY SHELLY JOHN
发明人： TAN WEIHONG , MECHERY SHELLY JOHN
CPC分类号： G01N21/6428 , G01N15/1459 , G01N2015/1486
摘要： The present invention provides systems for ultrasensitive detection of pathogens and bioparticles. One embodiment of the system comprises an optical detection scheme that allows for the detection of the fluorescence signal of bacteria or other bioparticles in less than about 20 minutes. A microflow channel allows for an assay probing volume of as little as a few picoliters. In one embodiment, the system uses RuBpy dye-doped silica nanoparticles bioconjugated with specific monoclonal antibodies of the target bioparticles. The system allows for the rapid and highly sensitive and specific detection of bacteria or other bioparticles without the need for amplication or enrichment of the sample.
摘要翻译：本发明提供了用于超灵敏检测病原体和生物颗粒的系统。该系统的一个实施方案包括允许在少于约20分钟内检测细菌或其他生物颗粒的荧光信号的光学检测方案。一个微流通道允许分析探测少至几微微升的体积。在一个实施方案中，该系统使用与靶生物颗粒的特异性单克隆抗体生物偶联的RuBpy染料掺杂二氧化硅纳米颗粒。该系统可以快速高灵敏度和特异性地检测细菌或其他生物颗粒，而无需扩增或富集样品。

8. 发明申请

WO2006074216A2 IDENTIFICATION OF MOLECULAR INTERACTIONS AND THERAPEUTIC USES THEREOF 审中-公开
标题翻译：分子相互作用的鉴定及其治疗用途
公开(公告)号：WO2006074216A2
公开(公告)日：2006-07-13
申请号：PCT/US2006000167
申请日：2006-01-04
申请人： UNIV FLORIDA , TAN WEIHONG , CAO ZEHUI
发明人： TAN WEIHONG , CAO ZEHUI
IPC分类号： C12Q1/68 , C12N15/11 , C12N15/115
CPC分类号： C12N15/111 , C12N15/115 , C12N2310/16 , C12N2310/3517 , C12N2320/10 , G01N33/5308 , G01N33/542
摘要： We report the real-time monitoring of protein-protein interactions without labeling either of the two interacting proteins, posing minimum effects on the binding properties of the proteins. In particular, the methods provide protein/aptamer complexes to probe the interactions in a competitive assay where the binding of an aptamer to its target protein is altered by a second protein that interacts with the target protein. Two signal transduction strategies, fluorescence resonance energy transfer (FRET) and fluorescence anisotropy, are described.
摘要翻译：我们报告实时监测蛋白质 - 蛋白质相互作用而不标记两种相互作用蛋白质中的任何一种，从而对蛋白质的结合性质产生最小影响。特别地，所述方法提供蛋白质/适体复合物以在竞争性测定中探测相互作用，其中适体与其靶蛋白的结合被与靶蛋白相互作用的第二蛋白改变。描述了两种信号转导策略，荧光共振能量转移（FRET）和荧光各向异性。

9. 发明申请

WO2011022291A3 PHOTOREGULATED REVERSIBLE HYDROGELS FOR DELIVERY AND RELEASING OF DRUGS AND OTHER THERAPEUTICAL REAGENTS 审中-公开
标题翻译：用于递送和释放药物和其他治疗试剂的光敏可逆的水凝胶
公开(公告)号：WO2011022291A3
公开(公告)日：2011-06-09
申请号：PCT/US2010045314
申请日：2010-08-12
申请人： UNIV FLORIDA , TAN WEIHONG , KANG HUAIZHI
发明人： TAN WEIHONG , KANG HUAIZHI
IPC分类号： A61K9/20 , A61K9/06 , A61K47/34 , A61K47/42 , A61K47/48
CPC分类号： A61K9/06 , A61K9/0009 , A61K41/0028 , A61K47/34
摘要： A novel hydrogel delivery systems useful for encapsulating and releasing pharmaceuticals or chemicals is disclosed where water soluble polymers containing crosslinker repeating units that associate or dissociate with complementary crosslinking repeating units or separate linkers to reversibly crosslink the hydrogel. In an exemplary embodiment, a DNA crosslinked hydrogel displays photoreversibility. An exemplary hydrogel delivery system comprises DNA polymer conjugates, wherein complementary DNA sequences are crosslinked with polymer chains and hybridization of the DNA sequences is controlled by photoresponsive moieties. Such hydrogcls can be used to release drug molecules and/or other therapeutic reagents. The exemplary hydrogel employs photosensitive azobenzene moieties that are incorporated into the DNA crosslinker units. The azobenzene moieties respond to different wavelengths of light so that the state of azobenzene isomerization is induced by the proportion of visible and UV light irradiated. The isomer state of the azobenzene dictates whether the complementary DNA sequences hybridize to cross link the DNA polymer conjugates. Thus, irradiation of light (visible or UV) can transform the hydrogel network between a sol and any of multiple gel states to regulate the degree of crosslinking between complementary DNA sequences and, therefore, provide a profile of release of a hydrogel encapsulated pharmaceutical or other chemical.
摘要翻译：公开了用于包封和释放药物或化学品的新型水凝胶递送系统，其中包含交联剂重复单元的水溶性聚合物，所述交联剂重复单元与互补交联重复单元或分开的接头缔合或解离以可逆地交联水凝胶。在一个示例性实施方案中，DNA交联水凝胶显示光可逆性。示例性水凝胶递送系统包含DNA聚合物缀合物，其中互补DNA序列与聚合物链交联并且DNA序列的杂交由光响应部分控制。这种水凝胶可用于释放药物分子和/或其他治疗剂。示例性水凝胶采用掺入DNA交联剂单元的光敏偶氮苯部分。偶氮苯部分对不同波长的光响应，以致偶氮苯异构化的状态由可见光和UV光照射的比例引起。偶氮苯的异构体状态指示互补DNA序列是否杂交以交联DNA聚合物缀合物。因此，光（可见光或UV）的照射可以在溶胶和多种凝胶状态中的任何一种之间转变水凝胶网络，以调节互补DNA序列之间的交联程度，并因此提供水凝胶包封的药物或其他化学。

10. 发明申请

WO2008134069A2 ANCHORED LIGAND AGENTS 审中-公开
标题翻译：安装的配对代理
公开(公告)号：WO2008134069A2
公开(公告)日：2008-11-06
申请号：PCT/US2008/005528
申请日：2008-04-28
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION , TAN, Weihong , CAO, Zehui , KIM, Youngmi
发明人： TAN, Weihong , CAO, Zehui , KIM, Youngmi
IPC分类号： A61K48/00
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/351 , C12N2320/50
摘要： The invention provides novel therapeutic agents comprising ligand molecules such as aptamers linked to a molecular anchor (MA). In the absence of a MA, ligands rapidly diffuse into bulk solution after dissociation from a target molecule. With a MA tightly bound to the target molecule, anchored ligand molecules of the invention remain in close proximity to the active site on the target molecule, even after dissociating from the target, permitting rapid re- binding of ligand to its target. Reversibility of binding to the active site can be effectively and rapidly achieved using antidotes based on the complementary DNA sequences. A preferred embodiment in accordance with the invention is a highly efficient anticoagulant agent that demonstrates binding efficiency to thrombin that is increased many fold relative to a corresponding unanchored aptamer-based ligand.
摘要翻译：本发明提供了包含配体分子的新型治疗剂，例如与分子锚（MA）连接的适体。在不存在MA的情况下，配体在从靶分子解离后迅速扩散到本体溶液中。使用与目标分子紧密结合的MA，本发明的锚定配体分子即使在与靶分离之后仍保持紧邻目标分子上的活性位点，从而允许配体快速重新结合至其靶。使用基于互补DNA序列的解毒剂可以有效快速地实现与活性位点结合的可逆性。根据本发明的优选实施方案是高效抗凝剂，其显示出相对于相应的未锚定的适体基配体增加多倍的凝血酶的结合效率。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式