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    • 6. 发明授权
    • Mammary gland tissue-specific expression system using &bgr;-casein promoter site of Korean native goat
    • 乳腺组织特异性表达系统使用韩国本地山羊的β-酪蛋白启动子位点
    • US06635474B1
    • 2003-10-21
    • US09582490
    • 2000-11-14
    • Ook Joon YooKyung Kwang LeeYoung Mahn HanSun Jung KimHae Young JeongJung Ho KoWon Jun Oh
    • Ook Joon YooKyung Kwang LeeYoung Mahn HanSun Jung KimHae Young JeongJung Ho KoWon Jun Oh
    • C12N1563
    • A01K67/0275A01K2217/05A01K2227/105A01K2267/01C07K14/4732C07K14/535C12N15/63C12N15/85C12N15/8509C12N2830/008C12N2830/42C12N2830/85
    • There are disclosed mammary gland tissue-specific expression systems using the promoter site for the &bgr;-casein gene of Korean native goats, by use of which physiological activating substances can be produced. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and pGbc_S (deposition Nos. KCTC 0515BP, 0514BP and 0513BP, respectively), a &bgr;-casein gene expression-regulating region, a physiological activating substance gene and a termination-regulating region are linked. Human granulocyte colony stimulating factor (hG-CSF) or human granulocyte macrophage colony stimulating factor (hGM-CSF) can be produced in HC11 cells, a mouse mammary gland tissue-derived cell line, and in the milk secreted from the transgenic mice by use of a hG-CSF or hGM-CSF gene-carrying pGbc, pGbc_L or pGbc_S in transfection into cell and microinjection to mouse. The proteins are those which experience the posttranslational modification and maintain their normal activity in the human body. The expression systems make it possible to easily produce the proteins at a great amount, to scale up protein production to the extent of industrialization, and to isolate and purify the desired protein with ease and safety.
    • 已经公开了使用韩国本地山羊的β-酪蛋白基因的启动子位点的乳腺组织特异性表达系统,通过使用可以产生生理活化物质。 在每个表达系统中,即新型质粒pGbc,pGbc_L和pGbc_S(分别为沉积编号KCTC 0515BP,0514BP和0513BP),β-酪蛋白基因表达调节区,生理活化物质基因和终止 - 调节区域相连。 人类粒细胞集落刺激因子(hG-CSF)或人粒细胞巨噬细胞集落刺激因子(hGM-CSF)可以在HC11细胞,小鼠乳腺组织来源的细胞系和转基因小鼠分泌的乳中产生 的hG-CSF或携带hGM-CSF基因的pGbc,pGbc_L或pGbc_S转染入细胞和显微注射到小鼠。 蛋白质是经历翻译后修饰并维持其在人体中的正常活性的蛋白质。 表达系统使得可以容易地大量生产蛋白质,将蛋白质生产扩大到工业化程度,并且容易且安全地分离和纯化所需的蛋白质。