专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2006149407A Dna encoding growth factor specific for epithelial cells 审中-公开
标题翻译： DNA编码上皮细胞特异性生长因子
公开(公告)号：JP2006149407A
公开(公告)日：2006-06-15
申请号：JP2006060217
申请日：2006-03-06
申请人： Stuart A Aaronson , Paul W Finch , Jeffrey S Rubin , ジェフェリー・エス・ルビン , スチュアート・エー・アーロンソン , ポール・ダブリュ・フィンチ
发明人： RUBIN JEFFREY S , FINCH PAUL W , AARONSON STUART A
IPC分类号： A61K39/395 , C12N15/09 , A61K38/00 , A61K38/18 , A61K38/22 , A61P17/00 , A61P17/02 , A61P43/00 , C07H21/04 , C07K1/16 , C07K14/00 , C07K14/435 , C07K14/47 , C07K14/475 , C07K14/485 , C07K14/50 , C07K16/00 , C07K16/22 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/08 , C12N5/10 , C12N15/12 , C12N15/18 , C12N15/63 , C12N15/70 , C12N15/79 , C12P21/00 , C12P21/02 , C12Q1/68 , C12R1/91 , G01N33/48 , G01N33/50 , G01N33/53
CPC分类号： C12Q1/6876 , A61K38/00 , C07K14/50 , C07K14/501 , C07K16/22 , C07K2317/73 , C07K2319/00 , C07K2319/02 , C07K2319/75 , C12N15/70 , C12N15/79 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a chimeric molecule comprising an amino acid sequence of KGF (keratin cell growth factor) and an amino acid sequence of at least one other member of FGF (fibroblast growth factor) peptide family. SOLUTION: A chimeric protein comprises following (1) and (2): (1) an amino acid sequence having selective mitogenic activity on epithelial cells and consisting of (a) an amino acid sequence represented by amino acid No. 32 to 64 and No. 157 to 161 in the following amino acid sequence or (b) an amino acid sequence represented by amino acid No. 32 to 64 in the following amino acid sequence and which is different in deletion of one or more amino acids contiguous from N-terminus; MHKWILTWILPTLLYRSCFHIICLVGTISLACNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT; and (2) at least one slice of the member of fibroblast growth factor family. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供包含KGF（角蛋白细胞生长因子）的氨基酸序列和FGF（成纤维细胞生长因子）肽家族的至少一种其他成员的氨基酸序列的嵌合分子。解决方案：嵌合蛋白包括以下（1）和（2）：（1）在上皮细胞上具有选择性促有丝分裂活性的氨基酸序列，由（a）氨基酸序列号32〜 64和157至161的氨基酸序列，或（b）下述氨基酸序列中的氨基酸32-64表示的氨基酸序列，并且与一个或多个与 N-末端; MHKWILTWILPTLLYRSCFHIICLVGTISLACNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT; 和（2）至少一片成纤维细胞生长因子家族成员。版权所有（C）2006，JPO＆NCIPI

2. 发明授权

US07427671B1 Compositions of alpha platelet derived growth factor receptor nucleic acid and protein and method of making 失效
标题翻译： α血小板衍生生长因子受体核酸和蛋白质的组成及其制备方法
公开(公告)号：US07427671B1
公开(公告)日：2008-09-23
申请号：US08439095
申请日：1995-05-11
申请人： Toshimitsu Matsui , Stuart A. Aaronson , Jacalyn H. Pierce
发明人： Toshimitsu Matsui , Stuart A. Aaronson , Jacalyn H. Pierce
IPC分类号： C07K14/71 , C07H21/04 , C12N15/12
CPC分类号： A61K51/103 , A61K38/00 , A61K2123/00 , C07K14/71 , C07K16/22 , C07K16/2863 , C07K2317/77 , C07K2319/30 , G01N33/74 , G01N2333/49 , G01N2333/71 , Y10S435/81
摘要： A DNA sequence which encodes a human type α platelet derived growth factor receptor protein which preferentially binds to the AA homodimer and AB heterodimer forms of platelet derived growth factor and also binds the BB homodimer at high affinity, is described. Substantially pure human a platelet derived growth factor receptor protein and methods for recombinantly producing human α platelet derived growth factor receptor protein are also described.
摘要翻译：描述了编码人类α血小板衍生生长因子受体蛋白的DNA序列，其优先结合AA同型二聚体和AB异二聚体形式的血小板衍生生长因子，并且还以高亲和力结合BB同二聚体。还描述了基本上纯的人α血小板衍生生长因子受体蛋白和用于重组产生人α血小板衍生生长因子受体蛋白的方法。

3. 发明授权

US07026291B1 Epithelial cell specific growth factor, keratinocyte growth factor (KGF) 失效
标题翻译：上皮细胞特异性生长因子，角质形成细胞生长因子（KGF）
公开(公告)号：US07026291B1
公开(公告)日：2006-04-11
申请号：US08477983
申请日：1995-06-07
申请人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
发明人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
IPC分类号： A61K38/18 , C07K14/475 , C07K14/50
CPC分类号： G01N33/74 , A61K38/00 , C07K14/50 , C07K16/22 , C07K2317/73 , G01N2333/4742
摘要： Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.
摘要翻译：揭示了显示重组DNA技术的特定方面的发现可以成功地用于产生迄今为止未知的人角质形成细胞生长因子（KGF）蛋白，而不含其他多肽。这些蛋白质可以从自发分泌的细胞或引入细胞的DNA片段以各种功能形式产生。这些形式不同地使得这种新型蛋白质的生物化学和功能研究以及抗体的产生。描述了用于确定KGF蛋白的基因表达水平的方法，例如通过测量细胞中的mRNA水平或通过测量在细胞外或体液中分泌的抗原。

4. 发明授权

US06228600B1 Immunoassays for the alpha platelet-derived growth factor receptor 失效
标题翻译： α血小板衍生生长因子受体的免疫测定
公开(公告)号：US06228600B1
公开(公告)日：2001-05-08
申请号：US08460656
申请日：1995-06-02
申请人： Toshimitsu Matsui , Stuart A. Aaronson , Jacalyn H. Pierce
发明人： Toshimitsu Matsui , Stuart A. Aaronson , Jacalyn H. Pierce
IPC分类号： G01N3353
CPC分类号： C07K14/71 , A61K38/00 , C07K16/22 , C07K16/2863 , C07K2319/30 , G01N33/74 , G01N2333/49 , G01N2333/71 , Y10T436/105831
摘要： Recombinant DNA technology was used to clone encoding nucleic acids for the human alpha platelet-derived growth factor receptor (PDGF-R type alpha). Peptides corresponding to the predicted sequence of this type and of the PDGF-R type beta were used to elicit antibodies specific for either type of the PDGF-R. Immunoassays are described for determining the level of PDGF-R type alpha in biological samples with the PDGF-R type alpha-specific antibodies.
摘要翻译：使用重组DNA技术来克隆用于人α血小板衍生生长因子受体（PDGF-R型α）的编码核酸。使用对应于这种类型的预测序列和PDGF-R型β的肽来引发对于任一种类型的PDGF-R特异性的抗体。描述了用PDGF-R型α特异性抗体测定生物样品中PDGF-R型α水平的免疫测定法。

5. 发明授权

US5707805A Assay for detecting keratinocyte growth factor (KGF) and its activity 失效
标题翻译：检测角质形成细胞生长因子（KGF）及其活性
公开(公告)号：US5707805A
公开(公告)日：1998-01-13
申请号：US455641
申请日：1995-05-31
申请人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
发明人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
IPC分类号： A61K39/395 , A61K38/00 , A61K38/18 , A61K38/22 , A61P17/02 , A61P43/00 , C07H21/04 , C07K1/16 , C07K14/00 , C07K14/435 , C07K14/47 , C07K14/475 , C07K14/485 , C07K14/50 , C07K16/00 , C07K16/22 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/08 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/18 , C12N15/63 , C12N15/70 , C12N15/79 , C12P21/00 , C12P21/02 , C12Q1/68 , C12R1/91 , G01N33/48 , G01N33/50 , G01N33/53
CPC分类号： C12Q1/6876 , C07K14/50 , C07K14/501 , C07K16/22 , C12N15/70 , C12N15/79 , A61K38/00 , C07K2317/73 , C07K2319/00 , C07K2319/02 , C07K2319/75 , C12Q2600/158
摘要： Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.
摘要翻译：揭示了显示重组DNA技术的特定方面的发现可以成功地用于产生迄今为止未知的人角质形成细胞生长因子（KGF）蛋白，而不含其他多肽。这些蛋白质可以从自发分泌的细胞或引入细胞的DNA片段以各种功能形式产生。这些形式不同地使得这种新型蛋白质的生物化学和功能研究以及抗体的产生。描述了用于确定KGF蛋白的基因表达水平的方法，例如通过测量细胞中的mRNA水平或通过测量在细胞外或体液中分泌的抗原。

6. 发明授权

US4820631A Deletion mutants and monoclonal antibodies against ras proteins 失效
标题翻译：缺失突变体和针对ras蛋白的单克隆抗体
公开(公告)号：US4820631A
公开(公告)日：1989-04-11
申请号：US890510
申请日：1986-07-30
申请人： Juan C. Lacal , Stuart A . Aaronson
发明人： Juan C. Lacal , Stuart A . Aaronson
IPC分类号： C07K14/82 , C07K16/32 , C12Q1/68 , C12N5/00 , G01N33/53 , G01N33/577
CPC分类号： C07K14/82 , C07K16/32 , Y10S435/81 , Y10S435/975 , Y10S436/808 , Y10S436/813
摘要： Specific deletion mutants of ras p21 gene and specific monoclonal antibodies which recognize specific regions of the ras p21 protein have been prepared. A kit for detecting the presence of specific ras p21 proteins and their levels in a body sample has been described.
摘要翻译：已经制备了ras p21基因的特异性缺失突变体和识别ras p21蛋白特异性区域的特异性单克隆抗体。已经描述了用于检测特定ras p21蛋白的存在及其在身体样品中的水平的试剂盒。

7. 发明授权

US07605127B2 Truncated hepatocyte growth factor variant protein HGF/NK2 有权
标题翻译：截短肝细胞生长因子变体蛋白HGF / NK2
公开(公告)号：US07605127B2
公开(公告)日：2009-10-20
申请号：US10283769
申请日：2002-10-30
申请人： Andrew M. L. Chan , Jeffrey S. Rubin , Donald P. Bottaro , Stuart A. Aaronson , Stephen J. Stahl , Paul T. Wingfield , Vittoria Cioce
发明人： Andrew M. L. Chan , Jeffrey S. Rubin , Donald P. Bottaro , Stuart A. Aaronson , Stephen J. Stahl , Paul T. Wingfield , Vittoria Cioce
IPC分类号： C07K14/475 , A61K38/18
CPC分类号： A61K38/193 , C07K14/4753 , A61K2300/00
摘要： The present invention relates to a novel truncated form of heptocyte growth factor (HGF) which specifically antagonizes the activity of HGF and to a novel truncated form of HGF that is a partial HGF agonist. In particular, the present invention relates to the purification, molecular cloning, recombinant expression of the truncated HGF variants and related pharmaceutical compositions. The present invention further relates to the utilization of the small HGF variants to either inhibit HGF mitogenesis or stimulate HGF mitogenesis in cells expressing the receptor for HGF.
摘要翻译：本发明涉及特异性拮抗HGF的活性的新型截短形式的肝细胞生长因子（HGF）和作为部分HGF激动剂的新型截短形式的HGF。特别地，本发明涉及截短的HGF变体和相关药物组合物的纯化，分子克隆，重组表达。本发明还涉及小HGF变体在表达HGF受体的细胞中抑制HGF有丝分裂或刺激HGF有丝分裂发生的用途。

8. 发明授权

US06709842B1 DNA encoding a growth factor specific for epithelial cells 失效
标题翻译：编码上皮细胞特异性生长因子的DNA
公开(公告)号：US06709842B1
公开(公告)日：2004-03-23
申请号：US08477982
申请日：1995-06-07
申请人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
发明人： Jeffrey S. Rubin , Paul W. Finch , Stuart A. Aaronson
IPC分类号： C12N121
CPC分类号： C12Q1/6876 , A61K38/00 , C07K14/50 , C07K14/501 , C07K16/22 , C07K2317/73 , C07K2319/00 , C07K2319/02 , C07K2319/75 , C12N15/70 , C12N15/79 , C12Q2600/158
摘要： Disccoveries are disclosed that show particular aspects of recombinant DNA technology can be used sucessfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.
摘要翻译：揭示了显示重组DNA技术的特定方面的Disccoveries可被成功地用于产生迄今为止未知的人角质形成细胞生长因子（KGF）蛋白，其不含其他多肽。这些蛋白质可以从自发分泌的细胞或引入细胞的DNA片段以各种功能形式产生。这些形式不同地使得这种新型蛋白质的生物化学和功能研究以及抗体的产生。描述了用于确定KGF蛋白的基因表达水平的方法，例如通过测量细胞中的mRNA水平或通过测量在细胞外或体液中分泌的抗原。

9. 发明授权

US06639060B1 erbB-3 nucleic acids 有权
标题翻译： erbB-3核酸
公开(公告)号：US06639060B1
公开(公告)日：2003-10-28
申请号：US09170699
申请日：1998-10-13
申请人： Matthias H. Kraus , Stuart A. Aaronson
发明人： Matthias H. Kraus , Stuart A. Aaronson
IPC分类号： C12N1511
CPC分类号： C07K16/32 , A61K38/00 , A61K47/6843 , C07K14/82 , C07K2317/34 , G01N33/5748 , G01N33/57484 , G01N2500/02 , G01N2500/04
摘要： A DNA fragment distinct from the epidermal growth factor receptor (EGFR) and erbB-2 genes was detected by reduced stringency hybridization of v-erbB to normal genomic human DNA. Characterization of the cloned DNA fragment mapped the region of v-erbB homology to three exons with closest homology of 64% and 67% to a contiguous region within the tyrosine kinase domains of the EGFR and erbB-2 proteins, respectively. cDNA cloning revealed a predicted 148 kd transmembrane polypeptide with structural features identifying it as a member of the erbB family, prompting designation of the new gene as erbB-3. It was mapped to human chromosome 12q11-13 and was shown to be expressed as a 6.2 kb transcript in a variety of normal tissues of epithelial origin. Markedly elevated erbB-3 mRNA levels were demonstrated in certain human mammary tumor cell lines. These findings indicate that increased erbB-3 expression, as in the case of EGFR and erbB-2, plays a role in some human malignancies. Using erbB-3 specific antibodies (polyclonal or monoclonal), the erbB-3 protein was identified as a 180 kDa glycoprotein, gp180erbB-3.
摘要翻译：通过v-erbB与正常基因组人DNA的严格杂交进行检测，检测出与表皮生长因子受体（EGFR）和erbB-2基因不同的DNA片段。克隆的DNA片段的表征将v-erbB同源性的区域分别与EGFR和erbB-2蛋白的酪氨酸激酶结构域内的连续区域分别具有64％和67％最近同源性的3个外显子。 cDNA克隆揭示了具有结构特征的预测的148kd跨膜多肽，其将其识别为erbB家族的成员，促使将新基因命名为erbB-3。它被映射到人染色体12q11-13，并且显示在上皮起源的多种正常组织中表达为6.2kb转录物。在某些人类乳腺肿瘤细胞系中证明了显着升高的erbB-3 mRNA水平。这些发现表明，如EGFR和erbB-2的情况，erbB-3表达的增加在一些人类恶性肿瘤中起作用。使用erbB-3特异性抗体（多克隆或单克隆），将erbB-3蛋白鉴定为180kDa糖蛋白gp180 。

10. 发明授权

US06403769B1 Fusion proteins that include antibody and nonantibody portions 失效
标题翻译：包含抗体和非抗体部分的融合蛋白
公开(公告)号：US06403769B1
公开(公告)日：2002-06-11
申请号：US08613743
申请日：1996-02-22
申请人： William J. Larochelle , Stuart A. Aaronson , Olaf Dirsch
发明人： William J. Larochelle , Stuart A. Aaronson , Olaf Dirsch
IPC分类号： C12P2108
CPC分类号： C07K14/71 , A61K38/00 , C07K16/00 , C07K2319/02
摘要： The high affinity which is characteristic of homodimers of IgG heavy chains is achieved, along with favorable secretion and flexibility/adaptability properties, in a fusion protein that has a nonantibody portion, comprised of an effector domain, joined to the aminoterminal end of an IgG-derived sequence consisting of a hinge:CH2:CH3 segment which lacks a CH1 domain, with a heterologous signal peptide preferably provided upstream of the nonantibody portion. Chimeric molecules of this structure can be secreted readily in stable form by mammalian cells transfected with DNA encoding the molecule, and are amenable to rapid, efficient purification to homogeneity, for example, using protein A. These molecules are effective substitutes for monoclonal antibodies in contexts such as flow cytometry, immunohistochemistry, immunoprecipitation and ELISAs. A fusion protein as described also can be used in screening for agonists and antagonists to the cognate binding partner of the nonantibody portion of the fusion protein. Moreover, chimeric molecules in which the nonantibody portion contains a growth factor domain are internalized, essentially like the natural growth factor, in contrast to the situation that generally pertains with respect to antibodies which are directed to external receptor domains.
摘要翻译：在具有非抗体部分的融合蛋白中实现了IgG重链的同源二聚体特征的高亲和力，以及有利的分泌和柔性/适应性特性，该融合蛋白包括与IgG-氨基末端的氨基末端结合的效应结构域，衍生的序列由铰链：缺少CH1结构域的CH2：CH3区段组成，异源信号肽优选设置在非抗体部分的上游。这种结构的嵌合分子可以通过用编码该分子的DNA转染的哺乳动物细胞以稳定的形式分泌，并且可以快速，有效地纯化成均匀性，例如使用蛋白质A.这些分子是上下文中单克隆抗体的有效替代物如流式细胞术，免疫组织化学，免疫沉淀和ELISA。还描述了融合蛋白，用于筛选融合蛋白非抗体部分的同源结合配偶体的激动剂和拮抗剂。此外，与通常涉及针对外部受体结构域的抗体的情况相反，非抗体部分含有生长因子结构域的嵌合分子基本上与天然生长因子内在化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式