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1. 发明专利

AU638042B2 MODIFIED PROTEASES AND THEIR USE IN FOODSTUFFS 未知
公开(公告)号：AU638042B2
公开(公告)日：1993-06-17
申请号：AU6051590
申请日：1990-08-02
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK (NIZO)
发明人： PETRUS ANTONIUS JOSEPHINA VOS , ROELANT JAN SIEZEN , WILLEM MEINDERT DE VOS , JAN KOK , GERHARDUS VENEMA , ALFRED JACQUES HAANDRIKMAN
IPC分类号： C12N15/09 , A23L27/20 , A23L27/21 , C12N1/21 , C12N9/52 , C12N15/57 , C12R1/225

2. 发明公开

EP0411715A2 Modified proteases, process for their preparation and their use in foodstuffs 失效
标题翻译：修饰的蛋白酶，它们的制备方法以及它们在食品的用途。
公开(公告)号：EP0411715A2
公开(公告)日：1991-02-06
申请号：EP90202113.8
申请日：1990-08-02
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK (NIZO)
发明人： Vos, Petrus Antonius Josephina , Siezen, Roelant Jan , De Vos, Willem Meindert , Kok, Jan , Venema, Gerhardus , Haandrikman, Alfred Jacques
IPC分类号： C12N15/57 , C12N15/62 , C12N15/74 , C12N1/21 , C12N9/52 , A23L1/226
CPC分类号： C12N9/52 , C07K2319/00
摘要： The invention relates to DNA fragments containing at least one "mutant protease gene", which "mutant protease gene" is understood to mean:
- a gene made up of sections of parent protease genes of several lactococcal strains, in particular of the parent protease genes of L. lactis subsp. cremoris Wg2 and L. lactis subsp. cremoris SK11, as well as
- a parent protease gene of a lactococcal strain, in particular the parent protease gene of L.lactis subsp. cremoris Wg2 or the parent protease gene of L.lactis subsp. cremoris SK11, the DNA sequence of which has been altered in a manner such that in the mutant protease for which the gene codes:

(a) an amino acid other than that of the "wild-type" protease is present at at least one site, and/or
(b) at at least one site within the first 1350 residues of the amino acid sequence, calculated from the N-terminus, at least one amino acid of the "wild-type" protease is missing and/or one or more amino acids have been inserted, or
(c) at at least two sites separated from each other, one or more amino acids are missing and/or one or more amino acids have been inserted,
the cloning vectors containing such a DNA fragment, the transformed host strains containing such a DNA fragment or vector, the mutant proteases obtainable as well as the foodstuffs and flavourings produced with the host strains or mutant proteases meant above.
摘要翻译：本发明涉及的DNA片段含有至少一个“突变体蛋白酶基因”，其中“突变体蛋白酶基因”是理解为是指： - 亲本蛋白酶的基因的由几乳球菌菌株的亲本蛋白酶的基因部分的基因，特别是的乳酸乳球菌亚种。乳脂WG2和乳酸乳球菌亚种。乳脂SK11，以及 - 一个应变乳球菌的亲本蛋白酶的基因，特别是乳酸乳球菌亚种的亲本蛋白酶的基因。乳脂W¯¯G2或乳酸乳球菌亚种的亲本蛋白酶的基因。比“野生型”的其它的（a）在氨基酸的蛋白酶是存在于至少一个位点：乳脂SK11，其中DNA序列已以这样的方式搜索被改变在突变体蛋白酶的量，基因编码没有和/或（b）在所述氨基酸序列的第1350个残基内的至少一个位点，从N-末端计算，“野生型”蛋白酶中的至少一个氨基酸被缺失和/或一个或多个氨基酸被插入，或（c）在彼此中，一个或多个氨基酸分开的至少两个位点缺失和/或一个或多个氨基酸被插入，含有这样的DNA片段的克隆载体，所述含寻求的DNA片段或载体转化的宿主菌株，产生具有上述指宿主菌株或突变型蛋白酶得到的突变型蛋白酶一个以及所述食品和调味剂。

3. 发明授权

EP1255446B1 STEAM HEATER 有权
标题翻译：蒸汽加热器
公开(公告)号：EP1255446B1
公开(公告)日：2005-11-30
申请号：EP01910220.1
申请日：2001-02-05
申请人： Stichting Nederlands Instituut Voor Zuivelonderzoek (NIZO)
发明人： DE JONG, Peter , VERDURMEN, Rudolph, Eduardus, Maria , STEENBERGEN, Albertus, Egbert
IPC分类号： A23C3/037 , A23L3/22
CPC分类号： A23C3/037 , A23L3/22 , Y10S261/10 , Y10S261/76
摘要： The invention relates to a device for treating a fluid product (4) such as a dairy product, with heat (6), the product (8) being transported with steam through a product conduit for treating the product with heat coming from the steam, the product treated with heat being subsequently supplied to an expansion tank (10) for cooling the product.

4. 发明授权

EP0579730B1 LANTIBIOTICS SIMILAR TO NISIN A, LACTIC ACID BACTERIA WHICH PRODUCE SUCH LANTIBIOTICS, METHOD FOR CONSTRUCTING SUCH LACTIC ACID BACTERIA AND METHOD FOR PRESERVING FOODSTUFFS WITH THE AID OF THESE LANTIBIOTICS AND THESE LACTIC ACID BACTERIA PRODUCING LANTIBIOTICS 失效
标题翻译：乳酸链球菌素类似羊毛硫这种制造乳杆菌及生产
公开(公告)号：EP0579730B1
公开(公告)日：2003-02-26
申请号：EP92910001.4
申请日：1992-04-09
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK (NIZO)
发明人： DE VOS, Willem, Meindert , SIEZEN, Roelant, Jan , KUIPERS, Oscar, Paul
IPC分类号： C12N15/74 , C07K14/00 , C12N1/21 , A23C19/097 , C12P21/02
CPC分类号： C12N15/746 , A23C19/11 , C07K14/315
摘要： The invention relates primarily to a number of lantibiotics similar to nisin A, which differ from nisin A by substitution, deletion or insertion of one or more amino acids, such as, in particular, the naturally occuring analogue of nisin A, termed nisin Z, which has the structure given above. Lantibiotics of this type are particularly suitable for preserving foodstuffs for humans and animals. This preservation takes place by the use of these lantibiotics themselves or of microorganisms producing these lantibiotics, in particular lactic acid bacteria, in the preparation or storage of foodstuffs.

5. 发明公开

EP0579730A1 LANTIBIOTICS SIMILAR TO NISIN A, LACTIC ACID BACTERIA WHICH PRODUCE SUCH LANTIBIOTICS, METHOD FOR CONSTRUCTING SUCH LACTIC ACID BACTERIA AND METHOD FOR PRESERVING FOODSTUFFS WITH THE AID OF THESE LANTIBIOTICS AND THESE LACTIC ACID BACTERIA PRODUCING LANTIBIOTICS 失效
标题翻译：乳酸链球菌素类似羊毛硫这种制造乳杆菌及其制备方法。
公开(公告)号：EP0579730A1
公开(公告)日：1994-01-26
申请号：EP92910001.0
申请日：1992-04-09
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK (NIZO)
发明人： DE VOS, Willem, Meindert , SIEZEN, Roelant, Jan , KUIPERS, Oscar, Paul
IPC分类号： A23C19 , C07K14 , C12N1 , C12N15
CPC分类号： C12N15/746 , A23C19/11 , C07K14/315
摘要： On décrit principalement un certain nombre de lantibiotiques semblables à la nisine A mais différents de celle-ci par substitution, la délétion ou l'insertion d'un ou plusieurs acides aminés, notamment l'analogue naturel de nisine A appelé nisine Z, dont la structure est présentée ci-dessus. Ce type de lantibiotiques est adapté notamment à la conservation des denrées alimentaires destinées à l'homme et aux animaux. Cette conservation peut s'effectuer lors de la préparation ou du stockage des denrées alimentaires, à l'aide de ces lantibiotiques proprement dits ou des microorganismes qui les produisent, notamment des bactéries de l'acide lactique.

6. 发明公开

EP0411715A3 Modified proteases, process for their preparation and their use in foodstuffs 失效
标题翻译：改性蛋白质，其制备方法及其在食品卫生中的应用
公开(公告)号：EP0411715A3
公开(公告)日：1991-04-10
申请号：EP90202113.8
申请日：1990-08-02
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK (NIZO)
发明人： Vos, Petrus Antonius Josephina , Siezen, Roelant Jan , De Vos, Willem Meindert , Kok, Jan , Venema, Gerhardus , Haandrikman, Alfred Jacques
IPC分类号： C12N15/57 , C12N15/62 , C12N15/74 , C12N1/21 , C12N9/52 , A23L1/226
CPC分类号： C12N9/52 , C07K2319/00
摘要： The invention relates to DNA fragments containing at least one "mutant protease gene", which "mutant protease gene" is understood to mean:
- a gene made up of sections of parent protease genes of several lactococcal strains, in particular of the parent protease genes of L. lactis subsp. cremoris Wg2 and L. lactis subsp. cremoris SK11, as well as
- a parent protease gene of a lactococcal strain, in particular the parent protease gene of L.lactis subsp. cremoris Wg2 or the parent protease gene of L.lactis subsp. cremoris SK11, the DNA sequence of which has been altered in a manner such that in the mutant protease for which the gene codes:
(a) an amino acid other than that of the "wild-type" protease is present at at least one site, and/or (b) at at least one site within the first 1350 residues of the amino acid sequence, calculated from the N-terminus, at least one amino acid of the "wild-type" protease is missing and/or one or more amino acids have been inserted, or (c) at at least two sites separated from each other, one or more amino acids are missing and/or one or more amino acids have been inserted, the cloning vectors containing such a DNA fragment, the transformed host strains containing such a DNA fragment or vector, the mutant proteases obtainable as well as the foodstuffs and flavourings produced with the host strains or mutant proteases meant above.

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