专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06864057B2 Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing 失效
标题翻译：从信使RNA构建单向克隆的cDNA文库，用于改进3'末端DNA测序
公开(公告)号：US06864057B2
公开(公告)日：2005-03-08
申请号：US10039890
申请日：2002-01-04
申请人： Glenn K. Fu , Steven Starnes , Laura L. Stuve
发明人： Glenn K. Fu , Steven Starnes , Laura L. Stuve
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12N15/1096
摘要： Methods are provide for preparing cDNA corresponding to a mRNA. In the subject methods, a mRNA is first contacted with a mixture of primers under first strand cDNA synthesis conditions. The primer mixture contains primers that have at least 10 contiguous deoxythymidines, a double stranded restriction enzyme recognition sequence near one end and a non-polyA-complementary region near the other end, where the non-polyA-complementary region is -VV, -VTV, -VTTV, -VTTTV, and -VVVVV. The resultant cDNA is modified such that the polyT tail is substantially removed. The modified cDNA is then ligated into a vector. The subject methods find use in a variety of applications, and find particular use in the sequencing of DNA and in the synthesis of cDNA libraries.
摘要翻译：提供制备对应于mRNA的cDNA的方法。在本发明方法中，首先在第一链cDNA合成条件下使mRNA与引物的混合物接触。引物混合物包含具有至少10个连续脱氧胸苷，一端附近的双链限制性酶识别序列和另一端附近的非聚-A互补区的引物，其中非聚-A互补区为-VV，-VTV ，-VTTV，-VTTTV和-VVVVV。所得cDNA被修饰，使得polyT尾部基本上被除去。然后将修饰的cDNA连接到载体中。本发明方法可用于各种应用，并且在DNA的测序和cDNA文库的合成中具有特别的用途。

2. 发明授权

US06387624B1 Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing 失效
标题翻译：从信使RNA构建单向克隆的cDNA文库，用于改进3'末端DNA测序
公开(公告)号：US06387624B1
公开(公告)日：2002-05-14
申请号：US09549770
申请日：2000-04-14
申请人： Glenn K. Fu , Steven Starnes , Laura L. Stuve
发明人： Glenn K. Fu , Steven Starnes , Laura L. Stuve
IPC分类号： C12Q168
CPC分类号： C12N15/1096
摘要： Methods are provide for preparing cDNA corresponding to a mRNA. In the subject methods, a mRNA is first contacted with a mixture of primers under first strand cDNA synthesis conditions. The primer mixture contains primers that have at least 10 contiguous deoxythymidines, a double stranded restriction enzyme recognition sequence near one end and a non-polyA-complementary region near the other end, where the non-polyA-complementary region is -VV, -VTV, -VTTV, -VTTTV, and -VVVVV. The resultant cDNA is modified such that the polyT tail is substantially removed. The modified cDNA is then ligated into a vector. The subject methods find use in a variety of applications, and find particular use in the sequencing of DNA and in the synthesis of cDNA libraries.
摘要翻译：提供制备对应于mRNA的cDNA的方法。在本发明方法中，首先在第一链cDNA合成条件下使mRNA与引物的混合物接触。引物混合物包含具有至少10个连续脱氧胸苷，一端附近的双链限制性酶识别序列和另一端附近的非聚-A互补区的引物，其中非聚-A互补区为-VV，-VTV ，-VTTV，-VTTTV和-VVVVV。所得cDNA被修饰，使得polyT尾部基本上被除去。然后将修饰的cDNA连接到载体中。本发明方法用于各种应用，并且在DNA的测序和cDNA文库的合成中具有特别的用途。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式