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    • 1. 发明授权
    • Banana DNA associated with fruit development
    • 与果实发育相关的香蕉DNA
    • US06284946B1
    • 2001-09-04
    • US09160351
    • 1998-09-25
    • Gregory D. MayStephanie K. Clendennen
    • Gregory D. MayStephanie K. Clendennen
    • C12N504
    • C12Y302/01073C07K14/415C07K14/42C07K14/825C12N9/0065C12N9/1051C12N9/2442C12N9/2448C12N15/8249C12N15/8257C12Y302/01014
    • The present invention provides isolated and purified genes which are differentially expressed during banana fruit development, and the protein products of these genes. The present invention further provides DNA regulatory elements which are differentially expressed during banana fruit development, chimeric genes comprising these DNA regulatory elements operably linked to heterologous DNA molecules, and plants transformed with said chimeric genes, providing for controlled expression of said heterologous DNA molecules during the development and ripening of the fruit of said plants, or in response to exogenous ethylene signals in said plants. The present invention also provides a method for expression of a heterologous protein in fruit comprising transforming fruiting plants with one or more chimeric genes according to the present invention, exposing said fruit to an endogenous or exogenous ethylene signal, and harvesting fruit containing said heterologous protein. The method of the present invention may further comprise isolated the proteins produced by said method from the harvested fruit. In a particularly preferred embodiment, the heterologous protein is a therapeutic protein, which may be isolated from the harvested fruit, or consumed directly in the transformed fruit by a patient in need of said therapeutic protein.
    • 本发明提供了在香蕉果实发育期间差异表达的分离和纯化的基因,以及这些基因的蛋白质产物。 本发明还提供了在香蕉果实发育期间差异表达的DNA调节元件,包含与异源DNA分子可操作地连接的这些DNA调节元件的嵌合基因,以及用所述嵌合基因转化的植物,提供所述异源DNA分子在 所述植物的果实的开发和成熟,或响应于所述植物中的外源乙烯信号。 本发明还提供了一种用于在果实中表达异源蛋白质的方法,包括用根据本发明的一种或多种嵌合基因转化结实植物,将所述果实暴露于内源或外源乙烯信号,以及收获含有所述异源蛋白质的水果。 本发明的方法还可以包括从收获的水果中分离由所述方法产生的蛋白质。 在特别优选的实施方案中,异源蛋白质是治疗性蛋白质,其可以从收获的果实中分离,或由需要所述治疗性蛋白质的患者直接在转化的果实中消耗。