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1. 发明申请

US20160138061A1 FATTY ACID AND DERIVATIVES PRODUCTION 审中-公开
标题翻译：脂肪酸和衍生物生产
公开(公告)号：US20160138061A1
公开(公告)日：2016-05-19
申请号：US14943333
申请日：2015-11-17
申请人： Thomas HAAS , Markus Poetter , Steffen Schaffer
发明人： Thomas HAAS , Markus Poetter , Steffen Schaffer
IPC分类号： C12P7/64
CPC分类号： C12P7/6409 , Y02P20/59
摘要： At least one fatty acid and/or derivative thereof is produced from a gas containing H2, CO2, and O2 by providing a genetically modified hydrogen oxidizing bacterium in an aqueous medium; and contacting the aqueous medium with the gas containing H2, CO2 and O2 in a weight ratio of 20 to 70 (H2): 10 to 45 (CO2): 5 to 35 (O2); wherein the fatty acid contains at least 5 carbon atoms and wherein the hydrogen oxidizing bacterium is genetically modified relative to the wild type bacterium to increase the expression of enzyme E1 that is capable of catalyzing the conversion of acetyl CoA to acyl ACP via malonyl coA and to increase the expression of enzyme E2 that is capable of catalyzing the conversion of Acyl ACP to the fatty acid.
摘要翻译：通过在水性介质中提供遗传修饰的氢氧化细菌，从含有H 2，CO 2和O 2的气体产生至少一种脂肪酸和/或其衍生物; 并使含水介质与含有H 2，CO 2和O 2的气体以20至70（H 2）：10至45（CO 2）：5至35（O 2）的重量比接触; 其中所述脂肪酸含有至少5个碳原子，并且其中所述氢氧化细菌相对于野生型细菌被遗传修饰以增加能够通过丙二酰辅酶A催化乙酰辅酶A转化为酰基ACP的酶E1的表达，增加能够催化酰基ACP向脂肪酸转化的酶E2的表达。

2. 发明授权

US09234222B2 Microbial strains producing sphingoid bases or derivatives thereof 有权
标题翻译：产生螺旋体碱或其衍生物的微生物菌株
公开(公告)号：US09234222B2
公开(公告)日：2016-01-12
申请号：US11666863
申请日：2005-11-07
申请人： Marco Alexander Van Den Berg , Steffen Schaffer
发明人： Marco Alexander Van Den Berg , Steffen Schaffer
IPC分类号： C12P13/00 , C12P13/02
CPC分类号： C12P13/001 , C12P13/02
摘要： The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii.
摘要翻译：本发明提供了生产至少0.1mg / g生物量干重的类鞘氨醇碱的微生物菌株，特别是酵母菌株。本发明还提供一种获得产生类鞘氨醇碱的微生物菌株的方法，包括在合适浓度的毒素存在下孵育一群微生物细胞，选择对所述毒素具有抗性的细胞，以及从毒素 - 产生至少0.1mg / g生物质干重的式I的类鞘氨醇碱。任选地，所述方法还包括使生产至少0.1mg / g生物量干重的毒素抗性微生物细胞群体式I的鞘氨醇类碱基与编码鞘脂代谢途径酶的多核苷酸进行DNA介导的转化。本发明还提供一种具有从毕赤酵母（Pichia ciferrii）获得的二氢神经酰胺去饱和酶活性的多肽。

3. 发明授权

US09150890B2 Candida tropicalis cells and use thereof 有权
公开(公告)号：US09150890B2
公开(公告)日：2015-10-06
申请号：US14609714
申请日：2015-01-30
申请人： Markus Poetter , Hans-Georg Hennemann , Steffen Schaffer , Thomas Haas
发明人： Markus Poetter , Hans-Georg Hennemann , Steffen Schaffer , Thomas Haas
IPC分类号： C12P7/62 , C12N1/16 , C12N9/02 , C12P7/42 , C12P13/04 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08
CPC分类号： C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要： The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

4. 发明申请

US20150111253A1 ENZYMATIC OMEGA-OXIDATION AND OMEGA-AMINATION OF FATTY ACIDS 有权
标题翻译：脂肪酸的氧化欧米茄氧化和欧米加氨基酸
公开(公告)号：US20150111253A1
公开(公告)日：2015-04-23
申请号：US14384301
申请日：2013-03-12
申请人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
发明人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
IPC分类号： C12P7/64 , C07K14/21
CPC分类号： C12P7/6436 , C07K14/21 , C07K14/79 , C12N9/0006 , C12N9/0016 , C12N9/0077 , C12N9/0095 , C12N9/1096 , C12P7/42 , C12P7/62 , C12P7/6409 , C12P13/005 , C12Y114/14001
摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.
摘要翻译：本发明涉及一种氧化式（I）的H3C-（CH2）n-COOR的脂肪酸或其酯的方法，其中R选自H，甲基，乙基，丙基和丁基，其中 n为0至30，优选6至24，包括通过在分子氧和NAD存在下使脂肪酸或其酯与CYP153家族的细胞色素P450单加氧酶接触来氧化脂肪酸或其酯的步骤 P）H和表达CYP153家族的重组细胞色素P450单加氧酶，重组醇脱氢酶，重组转氨酶以及任选的一种或多于一种重组酶的全细胞催化剂，其包含丙氨酸脱氢酶，铁氧还蛋白和铁氧还蛋白还原酶，以及所述全细胞催化剂用于氧化脂肪酸或其酯的用途。

5. 发明申请

US20140186905A1 BIOTECHNOLOGICAL SYNTHESIS PROCESS OF ORGANIC COMPOUNDS WITH THE AID OF AN ALKL GENE PRODUCT 审中-公开
标题翻译：有机化合物与ALKL基因产物的生物合成合成方法
公开(公告)号：US20140186905A1
公开(公告)日：2014-07-03
申请号：US14238576
申请日：2012-08-15
申请人： Steffen Schaffer , Jasmin Gielen , Nicole Decker , Nicole Kirchner , Thomas Haas , Markus Poetter , Harald Haeger
发明人： Steffen Schaffer , Jasmin Gielen , Nicole Decker , Nicole Kirchner , Thomas Haas , Markus Poetter , Harald Haeger
IPC分类号： C12P7/64 , C12P5/02 , C12P13/00
CPC分类号： C12P7/649 , C07K14/21 , C12P5/026 , C12P7/62 , C12P7/64 , C12P7/6409 , C12P13/001 , Y02E50/343 , Y02T50/678
摘要： Subject matter of the invention is a biotechnological process for the production of organic compounds with the aid of at least one alkL gene product.
摘要翻译：本发明的主题是借助至少一种alkL基因产物生产有机化合物的生物技术方法。

6. 发明申请

US20140178948A1 PRODUCTION OF OMEGA-AMINO FATTY ACIDS 有权
标题翻译：欧米茄 - 脂肪酸的生产
公开(公告)号：US20140178948A1
公开(公告)日：2014-06-26
申请号：US14132473
申请日：2013-12-18
申请人： Steffen SCHAFFER , Jasmin Gielen , Mirja Wessel , Hans-Georg Hennemann , Harald Haeger , Thomas Haas , Wilfried Bluemke
发明人： Steffen SCHAFFER , Jasmin Gielen , Mirja Wessel , Hans-Georg Hennemann , Harald Haeger , Thomas Haas , Wilfried Bluemke
IPC分类号： C12P13/00
CPC分类号： C12P13/001 , C12N9/0008 , C12N9/0016 , C12N9/0069 , C12N9/1096 , C12N9/1288 , C12P13/005 , Y02P20/582
摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.
摘要翻译：本发明提供一种全细胞催化剂，其表达重组α-加氧酶或重组脂肪酸还原酶与磷酸巯基乙酰转移酶的磷酸聚糖乙酰化脂肪酸还原酶的组合，除了α-加氧酶和/或脂肪酸的组合还原酶和磷酸泛酰乙酰转移酶表达转氨酶，其特征在于磷酸泛酰乙酰转移酶和/或转氨酶优选是重组的; 以及将脂肪酸ω-羟基脂肪酸，ω-氧代脂肪酸或其单酯转化成胺的方法，包括脂肪酸，ω-羟基脂肪酸，ω-氧代脂肪酸或其单酯通过与烷烃羟化酶和/或醇脱氢酶接触而与氧化产物接触，使氧化产物与磷酸泛酰乙酰化的脂肪酸还原酶或α-双加氧酶接触，得到醛，并使醛与转氨酶接触。

7. 发明申请

US20130164797A1 PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM 有权
标题翻译：从水质培养基中改善疏水有机溶液分离的方法
公开(公告)号：US20130164797A1
公开(公告)日：2013-06-27
申请号：US13721481
申请日：2012-12-20
申请人： Jasmin GIELEN , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
发明人： Jasmin GIELEN , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
IPC分类号： C12P7/64
CPC分类号： C12P7/6436 , C12N9/001 , C12P13/04 , C12Y103/99003
摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.
摘要翻译：提供了一种从水性培养基中改进疏水性有机溶液分离的方法。该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。代谢活性细胞的活性降低与野生型活性细胞相比，活性降低至少是一种催化脂肪酸β-氧化反应的一种酶。本发明还提供了一种代谢活性细胞，其与野生型相比具有降低的活性，所述活性细胞能催化脂肪酸的β-氧化反应之一，包括选自FadA，FadB，FadD， FadL和FadE及其变体。

8. 发明申请

US20130130319A1 CELLS AND METHODS FOR PRODUCING RHAMNOLIPIDS 有权
标题翻译：用于生产丹参酮的细胞和方法
公开(公告)号：US20130130319A1
公开(公告)日：2013-05-23
申请号：US13812625
申请日：2011-07-20
申请人： Steffen Schaffer , Mirja Wessel , Anja Thiessenhusen , Nadine Stein
发明人： Steffen Schaffer , Mirja Wessel , Anja Thiessenhusen , Nadine Stein
IPC分类号： C12P19/44
CPC分类号： C12N15/78 , A01N43/16 , C12N9/1051 , C12N9/14 , C12N15/52 , C12N15/70 , C12N15/77 , C12P19/44 , C12Y306/04013 , Y02P20/52
摘要： The invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids.
摘要翻译：本发明涉及细胞和核酸，以及其用于制备鼠李糖脂的用途，以及用于制备鼠李糖脂的方法。

9. 发明申请

US20100261237A1 FERMENTATIVE PRODUCTION OF ACETONE FROM RENEWABLE RESOURCES BY MEANS OF NOVEL METABOLIC PATHWAY 有权
标题翻译：通过新型代谢途径从可再生资源中发酵乙腈
公开(公告)号：US20100261237A1
公开(公告)日：2010-10-14
申请号：US12679488
申请日：2008-10-01
申请人： Stefan Verseck , Steffen Schaffer , Werner Freitag , Friedrich-Georg Schmidt , Matthias Orschel , Gerda Grund , Wilfried Schmidt , Hubert Johannes Bahl , Ralf-Joerg Fischer , Antje May , Peter Duerre , Simone Lederle
发明人： Stefan Verseck , Steffen Schaffer , Werner Freitag , Friedrich-Georg Schmidt , Matthias Orschel , Gerda Grund , Wilfried Schmidt , Hubert Johannes Bahl , Ralf-Joerg Fischer , Antje May , Peter Duerre , Simone Lederle
IPC分类号： C12P7/28 , C07H21/04 , C07C45/61 , C12N15/74
CPC分类号： C12P7/28 , C12N9/16 , C12N9/93 , C12Y301/02 , C12Y301/02018
摘要： The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO2, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.
摘要翻译：本发明描述了从乙酰辅酶A开始制备丙酮的方法，包括方法步骤A.将乙酰辅酶A酶促转化为乙酰乙酰辅酶A.将乙酰乙酰辅酶A酶促转化为乙酰乙酸酯，并将乙酰乙酸酯脱羧至丙酮， CO 2，其特征在于辅酶A在工艺步骤B中不转移到受体分子。此外，工艺步骤B由酰基-CoA硫酯酶，酰基辅酶A合成酶或酰基辅酶A硫代激酶类的催化剂惊人地催化。关注一个完全新颖的代谢途径，因为乙酰乙酰辅酶A的酶水解而不会同时转移CoA到受体分子，从未以前没有描述任何微生物酶。

10. 发明申请

US20080299625A1 Microbial Strains Producing Sphingoid Bases or Derivatives Thereof 有权
标题翻译：产生鞘氨醇碱或其衍生物的微生物菌株
公开(公告)号：US20080299625A1
公开(公告)日：2008-12-04
申请号：US11666863
申请日：2005-11-07
申请人： Marco Alexander Van Den Berg , Steffen Schaffer
发明人： Marco Alexander Van Den Berg , Steffen Schaffer
IPC分类号： C12P13/00 , C12N1/19 , C12N15/87 , C12N9/00 , C12N15/11
CPC分类号： C12P13/001 , C12P13/02
摘要： The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii.
摘要翻译：本发明提供了生产至少0.1mg / g生物量干重的类鞘氨醇碱的微生物菌株，特别是酵母菌株。本发明还提供一种获得产生类鞘氨醇碱的微生物菌株的方法，包括在合适浓度的毒素存在下孵育一群微生物细胞，选择对所述毒素具有抗性的细胞，以及从毒素 - 产生至少0.1mg / g生物质干重的式I的类鞘氨醇碱。任选地，所述方法还包括使生产至少0.1mg / g生物量干重的毒素抗性微生物细胞群体式I的鞘氨醇类碱基与编码鞘脂代谢途径酶的多核苷酸进行DNA介导的转化。本发明还提供一种具有从毕赤酵母（Pichia ciferrii）获得的二氢神经酰胺去饱和酶活性的多肽。

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