专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08097405B1 Nucleic acid sequencing processes using non-radioactive detectable modified or labeled nucleotides or nucleotide analogs, and other processes for nucleic acid detection and chromosomal characterization using such non-radioactive detectable modified or labeled nucleotides or nucleotide analogs 失效
标题翻译：使用非放射性可检测的修饰或标记核苷酸或核苷酸类似物的核酸测序方法，以及使用这种非放射性可检测修饰或标记核苷酸或核苷酸类似物的核酸检测和染色体表征的其他过程
公开(公告)号：US08097405B1
公开(公告)日：2012-01-17
申请号：US08486069
申请日：1995-06-07
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6816 , A61K47/62 , C07B59/008 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/6804 , C12Q2525/117
摘要： A process for determining the sequence of nucleic acids of interest employs nucleotides or nucleotide analogs that have been made detectable by non-radioactive modifying or labeling. Such nucleotides or nucleotide analogs are modified on the sugar moieties, the phosphate moieties or the base moieties, including base analogs. Modified nucleotide analogs can be attached to or coupled to or incorporated into DNA or RNA. The modified or labeled nucleotides or nucleotide analogs are also useful in processes for detecting the presence of nucleic acids of interest and for characterizing chromosomal sequences. Detection processes using the modified or labeled nucleotides or nucleotide analogs extend to the use of a gel for separating or resolving hybrids formed between non-radioactively labeled oligonucleotides or polynucleotides and such nucleic acids of interest.
摘要翻译：用于确定感兴趣的核酸序列的方法使用已经通过非放射性修饰或标记可检测的核苷酸或核苷酸类似物。这些核苷酸或核苷酸类似物在糖部分，磷酸酯部分或碱基部分（包括碱基类似物）上被修饰。修饰的核苷酸类似物可以连接到DNA偶联或并入DNA或RNA中。修饰或标记的核苷酸或核苷酸类似物也可用于检测感兴趣的核酸的存在和表征染色体序列的方法。使用经修饰或标记的核苷酸或核苷酸类似物的检测方法延伸到使用凝胶来分离或拆分在非放射性标记的寡核苷酸或多核苷酸与所关注的核酸之间形成的杂交体。

2. 发明授权

US06992180B1 Oligo- or polynucleotides comprising phosphate-moiety labeled nucleotides 失效
标题翻译：包含磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸
公开(公告)号：US06992180B1
公开(公告)日：2006-01-31
申请号：US08479997
申请日：1995-06-07
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Janes G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Janes G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： C12N15/11
CPC分类号： C12Q1/6816 , A61K47/62 , C07B59/008 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/6804 , C12Q2525/117
摘要： The present invention provides a nucleotide having the formula, Sig-PM-SM-BASE wherein PM is a phosphate moiety, SM is a sugar moiety and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached to the 3′ or the 5′ position of the sugar moiety when the nucleotide is a deoxyribonucleotide and at the 2′, 3′ or 5′ position when the nucleotide is a ribonucleotide. BASE is attached to the 1′ position of SM from the N1 position when BASE is a pyrmidine or the N9 position when BASE is a purine or 7-deazapurine. Sig is covalently attached to PM directly or via a chemical linkage, and represents a detectable moiety covalently attached to SM directly or through a linkage group. Also provided are an oligo- or polynucleotide comprising at least one such phosphate-moiety labeled nucleotide, and other compositions including those wherein a polypeptide is terminally ligated or attached to the oligo- or polynucleotide. The phosphate-moiety labeled nucleotide, and the oligo- or polynucleotides and other compositions comprising such phosphate-moiety labeled nucleotides, are useful as diagnostic tools for detecting analytes and as therapeutic agents.
摘要翻译：本发明提供了具有下式的核苷酸：<？in-line-formula description =“In-line Formulas”end =“lead”？> Sig-PM-SM-BASE <？in-line-formula description =“In 其中PM是磷酸酯部分，SM是糖部分，BASE是嘧啶，嘌呤或7-脱氮嘌呤部分。当核苷酸是脱氧核糖核苷酸时，当核苷酸是核糖核苷酸时，PM连接到糖部分的3'或5'位置，并且在2'，3'或5'位置。当BASE是嘌呤或7-脱氮嘌呤时，当BASE是嘧啶或N 9位时，BASE从N <1>位置连接到SM的1'位置。 Sig直接或通过化学键共价连接到PM，并且代表可直接或通过连接基共价连接到SM的可检测部分。还提供了包含至少一个这样的磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸，以及包括多肽终止连接或连接到寡核苷酸或多核苷酸的那些的其它组合物。磷酸酯部分标记的核苷酸以及包含这种磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸和其它组合物可用作检测分析物和作为治疗剂的诊断工具。

3. 发明授权

US07220854B1 Sugar moiety labeled nucleotide, and an oligo- or polynucleotide, and other compositions comprising such sugar moiety labeled nucleotides 失效
标题翻译：糖部分标记的核苷酸，以及寡核苷酸或多核苷酸，以及包含这种糖部分标记的核苷酸的其它组合物
公开(公告)号：US07220854B1
公开(公告)日：2007-05-22
申请号：US08486066
申请日：1995-06-07
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： C07H19/04
CPC分类号： C07H19/04
摘要： The present invention provides a nucleotide having the formula, wherein PM is a phosphate moiety, SM is a ribose or a deoxyribose sugar moiety, and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached to SM at a position independently selected from the 2′, 3′, and 5′ positions of SM when the nucleotide is a ribonucleotide, and at a position independently selected from the 3′ and 5′ positions when the nucleotide is a deoxyribonucleotide. BASE is attached to the 1′ position of SM from the N1 position when BASE is a pyrimidine, or the N9 position when BASE is a purine or 7-deazapurine. Sig is a detectable moiety covalently attached to SM directly or through a linkage group. Also provided are an oligo- or polynucleotide comprising at least one such sugar moiety labeled nucleotide, and other compositions including those wherein a polypeptide is terminal ligated or attached to the oligo- or polynucleotide. The sugar moiety labeled nucleotide, and the oligo- or polynucleotides and other compositions comprising such nucleotides, are useful as diagnostic tools for detecting analytes and as therapeutic agents.
摘要翻译：本发明提供了具有下式的核苷酸，其中PM是磷酸酯部分，SM是核糖或脱氧核糖糖部分，BASE是嘧啶，嘌呤或7-脱氮嘌呤部分。当核苷酸是核糖核苷酸时，PM与独立地选自SM的2'，3'和5'位置的位置连接，并且当该核苷酸为脱氧核糖核苷酸当BASE是嘧啶时，BASE从N 1位置连接到SM的1'位置，或当BASE是嘌呤或7-脱氮嘌呤时，BASE被连接到N 9的位置。 Sig是直接或通过连接基共价连接到SM的可检测部分。还提供了包含至少一个这样的糖部分标记的核苷酸的寡核苷酸或多核苷酸，以及其他组合物，包括其中多肽是末端连接或连接到寡核苷酸或多核苷酸的那些。标记核苷酸的糖部分和包含这种核苷酸的寡核苷酸和多核苷酸和其它组合物可用作检测分析物和作为治疗剂的诊断工具。

4. 发明申请

US20060160403A1 Wire tap connector and contact therefor 有权
公开(公告)号：US20060160403A1
公开(公告)日：2006-07-20
申请号：US11036750
申请日：2005-01-14
申请人： Scott Duesterhoeft , David Fabian , Stanley Kline
发明人： Scott Duesterhoeft , David Fabian , Stanley Kline
IPC分类号： H01R4/24
CPC分类号： H01R4/2433 , H01R4/4818
摘要： A contact for a wire tap connector includes a substantially planar insulation displacement portion configured to receive a primary wire and electrically connect thereto, and a poke in wire section integrally formed with and attached to the planar insulation displacement portion. The poke in wire section is configured to accept a prepared connecting wire in one or more positions relative to the insulation displacement portion. When the contact is situated in a housing, a slidable stuffer may be provided to quickly and easily install the connector assembly to a primary run wire.

5. 发明授权

US5384241A Specific binding assay compound with inhibitive self-quenching characteristics 失效
标题翻译：具有抑制自熄特性的特异性结合测定化合物
公开(公告)号：US5384241A
公开(公告)日：1995-01-24
申请号：US443812
申请日：1989-11-29
申请人： Stanley Kline
发明人： Stanley Kline
IPC分类号： C07H21/00 , C12Q1/68 , G01N33/542 , G01N33/58 , G01N33/53
CPC分类号： C07H21/00 , C12Q1/6816 , G01N33/542 , G01N33/582 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/827 , Y10S436/828 , Y10S530/802
摘要： Disclosed is an assay system including a compound comprising an analyte-specific moiety having substituted thereon a polymer comprising plurality of self-quenching emitter moieties and a plurality of isocharged functionality separating the emitter moieties. The present invention provides compounds that overcome the undesirable effects of self-quenching when multiple emitter moieties are used for labelling of assay reagents. Avoidance of this self-quenching phenomenon by the compounds of the invention makes it possible to introduce a more concentrated degree of labelling on to analyte-specific molecules such as oligo nucleotide probes, antibodies and other specific binding proteins and analyte-specific polysaccharides. Therefor, it is possible to effect greater assay sensitivity because the number of labels per recognition molecule(analyte-specific moiety) can be increased beyond the point previously possible without the reduction in signal caused by self-quenching.
摘要翻译：公开了一种测定系统，其包括包含分析物特异性部分的化合物，其在其上被取代了包含多个自熄发射体部分的聚合物和分离发射体部分的多个等电荷官能团。本发明提供了当多个发射体部分用于标记测定试剂时克服了自猝灭的不期望的影响的化合物。通过本发明的化合物避免这种自熄现象使得可以将更浓缩的标记度引入分析物特异性分子，例如寡核苷酸探针，抗体和其他特异性结合蛋白和分析物特异性多糖。因此，可以实现更高的测定灵敏度，因为每个识别分子（分析物特异性部分）的标签数量可以增加到先前可能的点之上，而不会由自淬灭引起的信号降低。

6. 发明授权

US5260433A Saccharide specific binding system labeled nucleotides 失效
标题翻译：糖类特异性结合系统标记核苷酸
公开(公告)号：US5260433A
公开(公告)日：1993-11-09
申请号：US567039
申请日：1990-08-13
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： A61K47/48 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/68 , C07H17/00
CPC分类号： C07K17/06 , A61K47/48238 , C07H19/04 , C07H19/10
摘要： The present invention provides nucleotides and polynucleotides which are chemically modified or labeled so as to be capable of ready detection when attached to and/or incorporated in nucleic acid material. More particularly, this invention provides a nucleotide selected from the group consisting of (i) a ribonucleotide having the formula, ##STR1## wherein PM is a phosphate moiety, SM is a sugar moiety, BASE is a pyrimidine, purine or 7-deazapurine moiety, and Sig is a saccharide moiety. PM is attached at the 2',3' or 5' position of SM, BASE is attached to the 1' position of SM from the N.sup.1 position when BASE is a pyrimidine or covalently attached to SM; and (ii) a nucleotide having the formula, ##STR2## wherein PM, SM, BASE and Sig are as defined above but PM is attached to the 3' or the 5' position of SM when said nucleotide is a deoxyribonucleotide and at the 2', 3' or 5' position when said nucleotide is a ribonucleotide, BASE is attached to the 1' position of SM from the N.sup.1 position when BASE is a pyrimidine or the N.sup.9 position when BASE is a purine, and wherein Sig is covalently attached to PM.
摘要翻译：本发明提供了化学修饰或标记的核苷酸和多核苷酸，以便在附着于和/或并入核酸材料时能够准确检测。更具体地，本发明提供了选自以下的核苷酸：（i）具有下式的核糖核苷酸，其中PM是磷酸酯部分，SM是糖部分，BASE是嘧啶嘌呤或7-脱氮嘌呤部分，Sig是糖部分。 PM连接在SM的2'，3'或5'位置，当BASE是嘧啶或共价连接到SM时，BASE从N1位置连接到SM的1'位置; 和（ii）具有下式的核苷酸，其中PM，SM，BASE和Sig如上所定义，但当所述核苷酸是脱氧核糖核苷酸时，PM连接到SM的3'或5'位，并且在2 当所述核苷酸为核糖核苷酸时，当碱基为嘧啶时，BASE与N1的1'位置连接，当BASE为嘌呤时，BASE与N9位置连接，并且其中Sig共价连接到PM。

7. 发明授权

US5241060A Base moiety-labeled detectable nucleatide 失效
标题翻译：碱基部分标记可检测核苷
公开(公告)号：US5241060A
公开(公告)日：1993-08-31
申请号：US532704
申请日：1990-06-04
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Jannis G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： A61K47/48 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/68 , G01N33/50
CPC分类号： C07H19/10 , A61K47/48238 , C07H19/04 , C07K17/06 , C12Q1/6804 , C12Q1/6816 , G01N33/50
摘要： The present invention provides nucleotides and polynucleotides which are chemically modified or labeled so as to be capable of ready detection when attached to and/or incorporated in nucleic acid material. More particularly, this invention provides a nucleotide having the formulaPM-SM-BASE-Sigwherein PM is a phosphate moiety, SM is a sugar moiety and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached at the 3' or the 5' position of SM when the nucleotide is a deoxyribonucleotide and at the 2', 3' or 5' position when the nucleotide is a ribonucleotide. BASE is attached to the 1' position of SM from the N.sup.1 position when BASE is a pyrimidine or the N.sup.9 position when BASE is a purine or a 7-deazapurine. Sig is a detectable moiety that is covalently attached to BASE at a position other than the C.sup.5 position when BASE is a pyrimidine, at a position other than the C.sup.8 position when BASE is a purine and at a position other than the C.sup.7 position when BASE is a 7-deazapurine.
摘要翻译：本发明提供了化学修饰或标记的核苷酸和多核苷酸，以便在附着于和/或并入核酸材料时能够准确检测。更具体地，本发明提供了具有式PM-SM-BASE-Sig的核苷酸，其中PM是磷酸酯部分，SM是糖部分，BASE是嘧啶，嘌呤或7-脱氮嘌呤部分。当核苷酸是脱氧核糖核苷酸时，当核苷酸是核糖核苷酸时，PM连接在SM的3'或5'位置处，并且在2'，3'或5'位置。当BASE是嘧啶时，BASE从N1位置连接到SM的1'位置，当BASE是嘌呤或7-脱氮嘌呤时，BASE连接到N9位置。当BASE是嘌呤时，当BASE是嘌呤时，除了C 8位以外的位置，当BASE是嘌呤时，Sig是可检测的部分，其在除了C5位置以外的位置处共价连接到BASE上，而当BASE是一种7-脱氮嘌呤。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式