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1. 发明授权

US06399340B1 Vector derivatives of gluconobacter plasmid pF4 失效
标题翻译：葡萄糖杆菌质粒pF4的载体衍生物
公开(公告)号：US06399340B1
公开(公告)日：2002-06-04
申请号：US09509565
申请日：2000-06-23
申请人： Yoshimasa Saito , Yuji Noguchi , Koji Yoshikawa , Shinsuke Soeda
发明人： Yoshimasa Saito , Yuji Noguchi , Koji Yoshikawa , Shinsuke Soeda
IPC分类号： C12P760
CPC分类号： C12N9/0006 , C12N15/74 , C12P7/60
摘要： A DNA derived from plasmid pF4, which contains a region involved in the control of autonomous replication in a bacterial cell belonging to the genus Gluconobactor, with or without a part of the polynucleotide region not essential for the autonomous replication therein, particularly, the same DNA comprising, as the region involved in the control of autonomous replication in the bacterial cell belonging to the genus Gluconobactor, a part or the entirety of the polynucleotide of nucleotide No. 2897-3969 region of Sequence Listing SEQ:ID No. 1. A plasmid vector containing this DNA, transformant transformed with this plasmid vector, and a method for producing a physiologically active substance comprising culturing this transformant. The full length nucleotide sequence of plasmid pF4 and the region containing DNA involved in the control of automonous replication in the bacterial cell belonging to the genus Gluconobactor were specified, whereby the region not essential for the automonous replication could be removed to provide a shortened pF4. The shortening of the region in the vector for autonomous replication increases the remaining region of the vector and allows incorporation of many structural genes into the vector. Consequently, the vector can have many functions.
摘要翻译：来自质粒pF4的DNA，其含有涉及控制属于Gluconobactor属的细菌细胞中的自主复制的区域，具有或不具有对其自主复制不是必需的一部分多核苷酸区域，特别是相同的DNA 包括作为控制属于Gluconobactor属的细菌细胞中的自主复制的区域的部分或全部是序列表SEQ ID NO：1的核苷酸2897-3969区域的多核苷酸的部分或全部。质粒含有该DNA的载体，用该质粒载体转化的转化体，以及生产生物活性物质的方法，包括培养该转化体。规定了质粒pF4的全长核苷酸序列和涉及控制属于Gluconobactor属细菌细胞中的自动复制控制的DNA的区域，由此可以除去对于自动复制不是必需的区域以提供缩短的pF4。自由复制载体中区域的缩短增加了载体的剩余区域，并允许将许多结构基因并入载体中。因此，矢量可以有很多功能。

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