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1. 发明申请

US20050053941A1 Extraction of nucleic acid 审中-公开
标题翻译：提取核酸
公开(公告)号：US20050053941A1
公开(公告)日：2005-03-10
申请号：US10496449
申请日：2002-11-20
申请人： Matthew Baker , Matthew Taylor , Shilpa Uppal
发明人： Matthew Baker , Matthew Taylor , Shilpa Uppal
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C12N1/08
CPC分类号： C12N15/1003 , C12Q1/6806
摘要： Methods of obtaining a sample of target nucleic acid from cells containing the target nucleic acid and genomic DNA or RNA are disclosed. In contrast to prior art protocols, this method does not require the cells containing the target nucleic acid to be lysed and instead is based on the observation when cells are suspended in an aqueous medium and the target nucleic acid are released into the medium through the cell walls. The invention therefore helps to avoid the use of cell lysis, heating, extremes of pH, water immiscible solvents, and electrical fields used in prior art nucleic acid extraction methods. The present invention is particularly applicable to the separation of non-genomic nucleic acid, such as cellular vector DNA or RNA, self-replicating satellite nucleic acids or plasmid DNA, from genomic nucleic acids, such as host cell chromosomes and ribosomal RNA.
摘要翻译：公开了从含有靶核酸和基因组DNA或RNA的细胞获得靶核酸样品的方法。与现有技术方案相反，该方法不需要含有靶核酸的细胞被裂解，而是基于当细胞悬浮在水性介质中并且靶核酸通过细胞释放到培养基中时的观察墙壁因此，本发明有助于避免使用现有技术的核酸提取方法中使用的细胞裂解，加热，极端pH，水不混溶溶剂和电场。本发明特别适用于从诸如宿主细胞染色体和核糖体RNA的基因组核酸分离非基因组核酸如细胞载体DNA或RNA，自复制卫星核酸或质粒DNA。

2. 发明申请

US20060263780A1 Tagged polyfunctional reagents capble of reversibly binding target substances in a ph-dependent manner 审中-公开
标题翻译：标记的多官能试剂以ph依赖的方式可逆地结合靶物质
公开(公告)号：US20060263780A1
公开(公告)日：2006-11-23
申请号：US10547644
申请日：2003-12-16
申请人： Matthew Baker , Matthew Taylor , Shilpa Uppal , Simon Douglas , Elliot Lawrence
发明人： Matthew Baker , Matthew Taylor , Shilpa Uppal , Simon Douglas , Elliot Lawrence
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N33/532 , C12N15/101 , G01N33/58 , Y10T436/13 , Y10T436/145555
摘要： Polyfunctional reagents are disclosed that are capable of reversibly binding to target substances, for example nucleic acid, proteins, polypeptides, cells, cell components, microorganisms or viruses, for use in purifying or otherwise manipulating them. The reagents comprise a tagging group for manipulating and/or detecting the target substance when bound to the polyfunctional reagent. The polyfunctional reagents work by binding the target substance at a first pH and then releasing it at a second pH, usually higher than the first. Examples of tagging groups include tagging group members of a specific binding pair which is capable of binding to a specific binding partner and/or a label.
摘要翻译：公开了能够可逆地结合目标物质（例如核酸，蛋白质，多肽，细胞，细胞成分，微生物或病毒）以用于纯化或以其他方式操作的多官能试剂。试剂包含用于在与多官能试剂结合时操纵和/或检测目标物质的标记基团。多官能试剂通过在第一pH下结合目标物质然后在通常高于第一pH的第二pH释放它来起作用。标记组的实例包括能够结合特异性结合配偶体和/或标签的特异性结合对的标记组成员。

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