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1. 发明申请

WO2012101998A1 METHOD FOR PRODUCTION OF RECOMBINANT HUMAN IDURONATE 2-SULFATASE 审中-公开
标题翻译：生产重组人胱天蛋白2-硫酸酯的方法
公开(公告)号：WO2012101998A1
公开(公告)日：2012-08-02
申请号：PCT/JP2012/000365
申请日：2012-01-23
申请人： JCR PHARMACEUTICALS CO., LTD. , MIHARA, Kazutoshi , KAWASAKI, Atsuko , OOTSUKI, Kouta , HATANO, Yuukichi , KAMEI, Shoichiro , SUGIMURA, Atsushi
发明人： MIHARA, Kazutoshi , KAWASAKI, Atsuko , OOTSUKI, Kouta , HATANO, Yuukichi , KAMEI, Shoichiro , SUGIMURA, Atsushi
IPC分类号： C12N15/09 , C12N9/16
CPC分类号： C12N9/16 , C12Y301/06013
摘要： Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.
摘要翻译：公开了大规模，高纯度和甘露糖6-磷酸残基生产重组人类伊朗阿拉酸2-硫酸酯酶（rhI2S）的方法。该方法包括以下步骤：（a）在无血清培养基中培养产生rhI2S的哺乳动物细胞，（b）收集培养上清，（c）使培养上清液进行阳离子交换柱层析，（d）染色亲和柱色谱法，（e）阴离子交换柱色谱法，和（f）以柱状色谱法，使用固相作为对磷酸酯基具有亲和性的材料，和（g）按凝胶过滤柱色谱法。

2. 发明申请

WO2014016873A1 METHOD FOR PRODUCTION OF RECOMBINANT HUMAN ALPHA-GALACTOSIDASE A 审中-公开
标题翻译：生产重组人α-半乳糖苷酶的方法A.
公开(公告)号：WO2014016873A1
公开(公告)日：2014-01-30
申请号：PCT/JP2012/004776
申请日：2012-07-26
申请人： JCR PHARMACEUTICALS CO., LTD. , ASANO, Masahiro , YAGI, Toshihiro , FUKUI, Tsuyoshi , KAWASAKI, Atsuko , HATANO, Yukichi , MIHARA, Kazutoshi , SUGIMURA, Atsushi
发明人： ASANO, Masahiro , YAGI, Toshihiro , FUKUI, Tsuyoshi , KAWASAKI, Atsuko , HATANO, Yukichi , MIHARA, Kazutoshi , SUGIMURA, Atsushi
IPC分类号： C12N9/40
CPC分类号： C12N9/2465
摘要： Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.
摘要翻译：公开了一种高纯度大规模生产重组人α-半乳糖苷酶A（rh-α-GalA）的方法。该方法包括以下步骤：（a）在无血清培养基中培养产生rh-α-Gal A的哺乳动物细胞，（b）收集培养上清液，（c）将培养上清液进行阴离子交换柱层析，（d）（e）在柱色谱上依次使用对磷酸基具有亲和性的物质，（f）阳离子交换柱色谱和（g）凝胶过滤柱色谱作为固相。 p>

3. 发明申请

WO1998008869A1 HEMATOPOIETIC STEM CELL GROWTH FACTOR (SCGF) 审中-公开
标题翻译： HEMATOPOIETIC STEM CELL GROWTH FACTOR（SCGF）
公开(公告)号：WO1998008869A1
公开(公告)日：1998-03-05
申请号：PCT/JP1997002985
申请日：1997-08-27
申请人： KYOWA HAKKO KOGYO CO., LTD. , HIRAOKA, Atsunobu , SUGIMURA, Atsushi , MIO, Hiroyuki
发明人： KYOWA HAKKO KOGYO CO., LTD.
IPC分类号： C07K14/52
CPC分类号： C07K14/475 , A61K38/00
摘要： A novel polypeptide exhibiting a growth promoting activity on hematopoietic stem cells; a gene encoding this polypeptide; an antibody reacting specifically with the polypeptide; a process for isolating the above-mentioned gene; and a vector to be used in this process. Thus, the pathologies of various hemopoietic organ diseases caused by abnormalities in hematopoietic cells and bone marrow inhibition can be clarified and, in its turn, these diseases can be diagnosed or treated. Also, it becomes possible to amplify hematopoietic cells in vitro for marrow graft required in treating these diseases or to elevate the gene transfer efficiency into hematopoietic stem cells for gene utilization. Moreover, the above-mentioned process and vector for isolating the SCGF gene are applicable to the clarification of other novel genes and thus contribute to the development of genetic engineering techniques.
摘要翻译：一种对造血干细胞具有生长促进活性的新型多肽; 编码该多肽的基因; 特异性与多肽反应的抗体; 分离上述基因的方法; 以及在此过程中使用的向量。因此，可以明确造血细胞异常引起的各种造血器官疾病和骨髓抑制的病态，从而可以诊断或治疗这些疾病。此外，可以在体外扩增用于治疗这些疾病所需的骨髓移植物的造血细胞，或者将基因转移效率提高到造血干细胞中用于基因利用。此外，用于分离SCGF基因的上述方法和载体可用于澄清其他新基因，从而有助于基因工程技术的发展。

4. 发明申请

WO2012101671A1 METHOD FOR PRODUCTION OF HUMAN RECOMBINANT IDURONATE 2-SULFATASE 审中-公开
标题翻译：生产人类重组蛋白2-硫酸酯的方法
公开(公告)号：WO2012101671A1
公开(公告)日：2012-08-02
申请号：PCT/JP2011/000392
申请日：2011-01-25
申请人： JCR PHARMACEUTICALS CO., LTD. , MIHARA, Kazutoshi , KAWASAKI, Atsuko , OOTSUKI, Kouta , HATANO, Yuukichi , KAMEI, Shoichiro , SUGIMURA, Atsushi
发明人： MIHARA, Kazutoshi , KAWASAKI, Atsuko , OOTSUKI, Kouta , HATANO, Yuukichi , KAMEI, Shoichiro , SUGIMURA, Atsushi
IPC分类号： C12N9/16 , C12N15/09
CPC分类号： C12N9/16 , C12Y301/06013
摘要： Disclosed is a method for production of human recombinant iduronate 2-sulfatase (hrI2S) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing hrI2S-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.
摘要翻译：公开了大规模，高纯度和甘露糖6-磷酸残基生产人重组糖尿病2-硫酸酯酶（hrI2S）的方法。该方法包括以下步骤：（a）在无血清培养基中培养产生hrI2S的哺乳动物细胞，（b）收集培养上清，（c）使培养上清液进行阳离子交换柱层析，（d）染色亲和柱色谱法，（e）阴离子交换柱色谱法，和（f）以柱状色谱法，使用固相作为对磷酸酯基具有亲和性的材料，和（g）按凝胶过滤柱色谱法。

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