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    • 2. 发明申请
      WO2006050257A3 DETECTION OF ION CHANNEL OR RECEPTOR ACTIVITY 审中-公开
    • DETECTION OF ION CHANNEL OR RECEPTOR ACTIVITY
    • 检测离子通道或受体活性
    • WO2006050257A3
    • 2009-05-07
    • PCT/US2005039260
    • 2005-10-31
    • MASSACHUSETTS INST OF TECCHNOL , CHILDRENS MEDICAL CENTER , MARINI DAVIDE M , DESAI BIMAL , DELLING MARKUS , SOLIS DANIEL J , BELCHER ANGELA M , CLAPHAM DAVID E , FEBVAY SEBASTIEN , CARTER BRETT
    • MARINI DAVIDE M , DESAI BIMAL , DELLING MARKUS , SOLIS DANIEL J , BELCHER ANGELA M , CLAPHAM DAVID E , FEBVAY SEBASTIEN , CARTER BRETT
    • G01N33/551
    • C12Q1/005 , B82Y5/00 , B82Y10/00 , G01N21/554 , G01N33/542 , G01N33/54373 , G01N33/6872 , G01N2333/726
    • The invention provides nanosensors and nanosensor components for the detection of ion channel activity, receptor activity, or protein protein interactions. Certain of the nanosensor components comprise a nanoparticle and recognition domain. Following contact with cells and, optionally, internalization of the nanosensor component by a cell, the recognition domain binds to a target domain, e.g., a heterologous target domain, of a polypeptide of interest such as an ion channel subunit, G protein coupled receptor (GPCR), or G protein subunit. Ion channel activity, GPCR activity, or altered protein interaction results in a detectable signal. The nanoparticles may be functionalized so that they respond to the presence of an ion by altering their proximity. Certain of the nanosensors utilize the phenomenon of plasmon resonance to produce a signal while others utilize magnetic properties, RET, and/or ion-sensitive moieties. Also provided are polypeptides, e.g., ion channel subunits, comprising a heterologous target domain, and cell lines that express the polypeptides. Further provided are a variety of methods for detecting ion channel activity, receptor activity, or protein interaction and for identifying compounds that modulate one or more of these. In certain embodiments the invention allows the user to detect the activity of specific ion channels even in the presence of other channels that permit passage of the same ion(s) or result in activation of the same downstream targets, thereby achieving improved specificity in high throughput screens while at the same time providing a high signal to noise ratio.
    • 本发明提供用于检测离子通道活性，受体活性或蛋白质蛋白相互作用的纳米传感器和纳米传感器组分。 某些纳米传感器组件包含纳米颗粒和识别结构域。 在与细胞接触并且任选地，通过细胞使纳米传感器组分内化后，识别结构域结合感兴趣的多肽的靶结构域例如异源靶结构域，例如离子通道亚基，G蛋白偶联受体（ GPCR）或G蛋白亚基。 离子通道活性，GPCR活性或改变的蛋白质相互作用导致可检测的信号。 纳米颗粒可以被官能化，使得它们通过改变它们的接近度来响应离子的存在。 某些纳米传感器利用等离子体共振现象来产生信号，而另一些利用磁性，RET和/或离子敏感部分。 还提供了多肽，例如包含异源靶域的离子通道亚单位，以及表达多肽的细胞系。 进一步提供了用于检测离子通道活性，受体活性或蛋白质相互作用以及鉴定调节这些中的一种或多种的化合物的各种方法。 在某些实施方案中，本发明允许用户即使在允许相同离子通过或导致相同下游靶的活化的其它通道的存在下也检测特定离子通道的活性，从而实现高通量的改善的特异性 屏幕同时提供高信噪比。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 6. 发明申请
      WO2005067683A8 INORGANIC NANOWIRES 审中-公开
    • INORGANIC NANOWIRES
    • 无机纳米级
    • WO2005067683A8
    • 2006-12-14
    • PCT/US2005000075
    • 2005-01-05
    • UNIV TEXAS , MASSACHUSETTS INST TECHNOLOGY , BELCHER ANGELA M , MAO CHUANBIN , SOLIS DANIEL J
    • BELCHER ANGELA M , MAO CHUANBIN , SOLIS DANIEL J
    • D02G3/00 , C04B35/64 , H01L29/06 , H01L29/22 , H01L29/745 , H05B6/00
    • H01B1/10 , B82Y10/00 , B82Y30/00 , C01B19/007 , C01G9/08 , C01P2004/04 , C01P2004/16 , C04B35/62272 , C04B2235/3284 , C04B2235/446 , H01B1/06 , H01F1/01 , H01F1/068 , H01L29/0665 , H01L29/0669 , H01L29/0673 , H01L29/22 , Y10T428/249924 , Y10T428/2913 , Y10T428/2927 , Y10T428/298
    • An inorganic nanowire having an organic scaffold substantially removed from the inorganic nanowire, the inorganic nanowire consisting essentially of fused inorganic nanoparticles substantially free of the organic scaffold, and methods of making same. For example, a virus-based scaffold for the synthesis of single crystal ZnS, CdS and free-standing L10 CoPt and FePt nanowires can be used, with the means of modifying substrate specificity through standard biological methods. Peptides can be selected through an evolutionary screening process that exhibit control of composition, size, and phase during nanoparticle nucleation have been expressed on the highly ordered filamentous capsid of the M13 bacteriophage. The incorporation of specific, nucleating peptides into the generic scaffold of the M13 coat structure can provide a viable template for the directed synthesis of a variety of materials including semiconducting and magnetic materials. Removal of the viral template via annealing can promote oriented aggregation-based crystal growth, forming individual crystalline nanowires. The unique ability to interchange substrate specific peptides into the linear self­assembled filamentous construct of the M 13 virus introduces a material tunability not seen in previous synthetic routes. Therefore, this system provides a genetic tool kit for growing and organizing nanowires from various materials including semiconducting and magnetic materials.
    • 具有基本上从无机纳米线除去的有机支架的无机纳米线，基本上不含有机支架的熔融无机纳米线组成的无机纳米线及其制备方法。 例如，可以使用用于合成单晶ZnS，CdS和独立的L10 CoPt和FePt纳米线的基于病毒的支架，具有通过标准生物学方法修饰底物特异性的方法。 可以通过进化筛选方法选择肽，其在M13噬菌体的高度有序的丝状衣壳上表达纳米颗粒成核期间组合物，大小和相位的控制。 将特定的成核肽并入M13涂层结构的通用支架中可以提供用于定向合成各种材料（包括半导体和磁性材料）的可行模板。 通过退火去除病毒模板可促进取向聚集的晶体生长，形成单独的晶体纳米线。 将底物特异性肽转化为M13病毒的线性自组装丝状构建体的独特能力引入了在以前的合成途径中未见的材料可调性。 因此，该系统提供了一种遗传工具包，用于从包括半导体和磁性材料的各种材料生长和组织纳米线。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 7. 发明申请
      WO2005067683A2 INORGANIC NANOWIRES 审中-公开
    • INORGANIC NANOWIRES
    • 无机纳米材料
    • WO2005067683A2
    • 2005-07-28
    • PCT/US2005/000075
    • 2005-01-05
    • BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM? , MASSACHUSETTS INSTITUTE OF TECHNOLOGY, INC. , BELCHER, Angela, M. , MAO, Chuanbin , SOLIS, Daniel, J.
    • BELCHER, Angela, M. , MAO, Chuanbin , SOLIS, Daniel, J.
    • C04B35/64 , D02G3/00 , H01L29/06 , H01L29/22 , H01L29/745 , H05B6/00
    • H01B1/10 , B82Y10/00 , B82Y30/00 , C01B19/007 , C01G9/08 , C01P2004/04 , C01P2004/16 , C04B35/62272 , C04B2235/3284 , C04B2235/446 , H01B1/06 , H01F1/01 , H01F1/068 , H01L29/0665 , H01L29/0669 , H01L29/0673 , H01L29/22 , Y10T428/249924 , Y10T428/2913 , Y10T428/2927 , Y10T428/298
    • An inorganic nanowire having an organic scaffold substantially removed from the inorganic nanowire, the inorganic nanowire consisting essentially of fused inorganic nanoparticles substantially free of the organic scaffold, and methods of making same. For example, a virus-based scaffold for the synthesis of single crystal ZnS, CdS and free-standing L10 CoPt and FePt nanowires can be used, with the means of modifying substrate specificity through standard biological methods. Peptides can be selected through an evolutionary screening process that exhibit control of composition, size, and phase during nanoparticle nucleation have been expressed on the highly ordered filamentous capsid of the M13 bacteriophage. The incorporation of specific, nucleating peptides into the generic scaffold of the M13 coat structure can provide a viable template for the directed synthesis of a variety of materials including semiconducting and magnetic materials. Removal of the viral template via annealing can promote oriented aggregation-based crystal growth, forming individual crystalline nanowires. The unique ability to interchange substrate specific peptides into the linear self­assembled filamentous construct of the M 13 virus introduces a material tunability not seen in previous synthetic routes. Therefore, this system provides a genetic tool kit for growing and organizing nanowires from various materials including semiconducting and magnetic materials.
    • 具有基本上从无机纳米线去除的有机支架的无机纳米线，所述无机纳米线基本上由基本上不含有机支架的融合无机纳米粒子组成，以及制备所述无机纳米线的方法。 例如，可以使用基于病毒的用于合成单晶ZnS，CdS和独立式L10 CoPt和FePt纳米线的支架，以及通过标准生物学方法改变​​底物特异性的手段。 可以通过进化筛选过程来选择肽，在M13噬菌体的高度有序丝状衣壳上已经表达了纳米颗粒成核过程中组成，大小和相的控制。 将特定的成核肽掺入到M13涂层结构的通用支架中可以为包括半导体和磁性材料在内的各种材料的定向合成提供可行的模板。 通过退火去除病毒模板可以促进基于聚集的定向晶体生长，从而形成单独的晶体纳米线。 将底物特异性肽交换成M13病毒的线性自身和害羞组装丝状构建体的独特能力引入了在先前的合成途径中未见的材料可调性。 因此，这个系统提供了一个基因工具包，用于从各种材料中生长和组织纳米线，包括半导体和磁性材料。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 8. 发明申请
      WO2006050257A2 DETECTION OF ION CHANNEL OR RECEPTOR ACTIVITY 审中-公开
    • DETECTION OF ION CHANNEL OR RECEPTOR ACTIVITY
    • 检测离子通道或受体活性
    • WO2006050257A2
    • 2006-05-11
    • PCT/US2005/039260
    • 2005-10-31
    • MASSACHUSETTS INSTITUTE OF TECCHNOLOGY , CHILDREN'S MEDICAL CENTER CORPORATION , HOWARD HUGHES MEDICAL INSTITUTE , MARINI, Davide, M. , DESAI, Bimal , DELLING, Markus , SOLIS, Daniel, J. , BELCHER, Angela, M. , CLAPHAM, David, E. , FEBVAY, Sebastien , CARTER, Brett
    • MARINI, Davide, M. , DESAI, Bimal , DELLING, Markus , SOLIS, Daniel, J. , BELCHER, Angela, M. , CLAPHAM, David, E. , FEBVAY, Sebastien , CARTER, Brett
    • G01N33/53
    • C12Q1/005 , B82Y5/00 , B82Y10/00 , G01N21/554 , G01N33/542 , G01N33/54373 , G01N33/6872 , G01N2333/726
    • The invention provides nanosensors and nanosensor components for the detection of ion channel activity, receptor activity, or protein protein interactions. Certain of the nanosensor components comprise a nanoparticle and recognition domain. Following contact with cells and, optionally, internalization of the nanosensor component by a cell, the recognition domain binds to a target domain, e.g., a heterologous target domain, of a polypeptide of interest such as an ion channel subunit, G protein coupled receptor (GPCR), or G protein subunit. Ion channel activity, GPCR activity, or altered protein interaction results in a detectable signal. The nanoparticles may be functionalized so that they respond to the presence of an ion by altering their proximity. Certain of the nanosensors utilize the phenomenon of plasmon resonance to produce a signal while others utilize magnetic properties, RET, and/or ion-sensitive moieties. Also provided are polypeptides, e.g., ion channel subunits, comprising a heterologous target domain, and cell lines that express the polypeptides. Further provided are a variety of methods for detecting ion channel activity, receptor activity, or protein interaction and for identifying compounds that modulate one or more of these. In certain embodiments the invention allows the user to detect the activity of specific ion channels even in the presence of other channels that permit passage of the same ion(s) or result in activation of the same downstream targets, thereby achieving improved specificity in high throughput screens while at the same time providing a high signal to noise ratio.
    • 本发明提供用于检测离子通道活性，受体活性或蛋白质蛋白相互作用的纳米传感器和纳米传感器组分。 某些纳米传感器组件包含纳米颗粒和识别结构域。 在与细胞接触并且任选地，通过细胞使纳米传感器组分内化后，识别结构域结合感兴趣的多肽的靶结构域例如异源靶结构域，例如离子通道亚基，G蛋白偶联受体（ GPCR）或G蛋白亚基。 离子通道活性，GPCR活性或改变的蛋白质相互作用导致可检测的信号。 纳米颗粒可以被官能化，使得它们通过改变它们的接近度来响应离子的存在。 某些纳米传感器利用等离子体共振现象来产生信号，而另一些利用磁性，RET和/或离子敏感部分。 还提供了多肽，例如包含异源靶域的离子通道亚单位，以及表达多肽的细胞系。 进一步提供了用于检测离子通道活性，受体活性或蛋白质相互作用以及鉴定调节这些中的一种或多种的化合物的各种方法。 在某些实施方案中，本发明允许用户即使在允许相同离子通过或导致相同下游靶的活化的其它通道的存在下也检测特定离子通道的活性，从而实现高通量的改善的特异性 屏幕同时提供高信噪比。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 9. 发明申请
      WO2005067683A3 INORGANIC NANOWIRES 审中-公开
    • INORGANIC NANOWIRES
    • 无机纳米级
    • WO2005067683A3
    • 2005-12-08
    • PCT/US2005000075
    • 2005-01-05
    • UNIV TEXAS , MASSACHUSETTS INST TECHNOLOGY , BELCHER ANGELA M , MAO CHUANBIN , SOLIS DANIEL J
    • BELCHER ANGELA M , MAO CHUANBIN , SOLIS DANIEL J
    • C04B35/64 , D02G3/00 , H01L29/06 , H01L29/22 , H01L29/745 , H05B6/00
    • H01B1/10 , B82Y10/00 , B82Y30/00 , C01B19/007 , C01G9/08 , C01P2004/04 , C01P2004/16 , C04B35/62272 , C04B2235/3284 , C04B2235/446 , H01B1/06 , H01F1/01 , H01F1/068 , H01L29/0665 , H01L29/0669 , H01L29/0673 , H01L29/22 , Y10T428/249924 , Y10T428/2913 , Y10T428/2927 , Y10T428/298
    • An inorganic nanowire having an organic scaffold substantially removed from the inorganic nanowire, the inorganic nanowire consisting essentially of fused inorganic nanoparticles substantially free of the organic scaffold, and methods of making same. For example, a virus-based scaffold for the synthesis of single crystal ZnS, CdS and free-standing L10 CoPt and FePt nanowires can be used, with the means of modifying substrate specificity through standard biological methods. Peptides can be selected through an evolutionary screening process that exhibit control of composition, size, and phase during nanoparticle nucleation have been expressed on the highly ordered filamentous capsid of the M13 bacteriophage. The incorporation of specific, nucleating peptides into the generic scaffold of the M13 coat structure can provide a viable template for the directed synthesis of a variety of materials including semiconducting and magnetic materials. Removal of the viral template via annealing can promote oriented aggregation-based crystal growth, forming individual crystalline nanowires. The unique ability to interchange substrate specific peptides into the linear self­assembled filamentous construct of the M 13 virus introduces a material tunability not seen in previous synthetic routes. Therefore, this system provides a genetic tool kit for growing and organizing nanowires from various materials including semiconducting and magnetic materials.
    • 具有基本上从无机纳米线除去的有机支架的无机纳米线，基本上不含有机骨架的熔融无机纳米线组成的无机纳米线及其制备方法。 例如，可以使用用于合成单晶ZnS，CdS和独立的L10 CoPt和FePt纳米线的基于病毒的支架，具有通过标准生物学方法修饰底物特异性的方法。 可以通过进化筛选方法选择肽，其在M13噬菌体的高度有序的丝状衣壳上表达纳米颗粒成核期间组合物，大小和相位的控制。 将特定的成核肽并入M13涂层结构的通用支架中可以提供用于定向合成各种材料（包括半导体和磁性材料）的可行模板。 通过退火去除病毒模板可促进取向聚集的晶体生长，形成单独的晶体纳米线。 将底物特异性肽转化为M13病毒的线性自组装丝状构建体的独特能力引入了在以前的合成途径中未见的材料可调性。 因此，该系统提供了一种遗传工具包，用于从包括半导体和磁性材料的各种材料生长和组织纳米线。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
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