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1. 发明申请

WO2011004994A3 A NOVEL GENOME WALKING METHOD FOR CLONING OF UNKNOWN DNA SEQUENCES ADJACENT TO KNOWN SEQUENCE 审中-公开
公开(公告)号：WO2011004994A3
公开(公告)日：2011-01-13
申请号：PCT/KR2010/004365
申请日：2010-07-06
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , SOHN, Jung-Hoon , BAE, Jung-Hoon
发明人： SOHN, Jung-Hoon , BAE, Jung-Hoon
IPC分类号： C12Q1/68 , C12N15/11 , C07H21/00
摘要： The present invention relates to a novel genome walking method for cloning unknown genomic regions adjacent to known sequences by PCR. More particularly, the present invention relates to a method for cloning an unknown nucleotide sequence adjacent to a known nucleotide sequence, comprising the steps of 1) cleaving a genomic DNA that contains a known nucleotide sequence and an unknown nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3'-OH end of the cleaved DNA with a nucleotide analogue; 3) linking the terminal sequence of the cleaved DNA fragment with a linker, adapter or cassette having a terminal sequence complementary thereto; and 4) amplifying the unknown nucleotide sequence using a known nucleotide sequence-specific primer and a cassette sequence-specific primer. Further, the present invention relates to a composition of gene amplification for cloning the unknown nucleotide sequence, and a gene amplification kit comprising the composition.

2. 发明申请

WO2007015178A3 LIBRARY OF TRANSLATIONAL FUSION PARTNERS FOR PRODUCING RECOMBINANT PROTEINS AND TRANSLATIONAL FUSION PARTNERS SCREENED THEREFROM 审中-公开
公开(公告)号：WO2007015178A3
公开(公告)日：2007-02-08
申请号：PCT/IB2006/003102
申请日：2006-07-13
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , SOHN, Jung-Hoon , CHOI, Eui-Sung , BAE, Jung-Hoon , SHIN, Mi-Kyung , YOON, Sung-Sook , CHUN, Chang-Soo
发明人： SOHN, Jung-Hoon , CHOI, Eui-Sung , BAE, Jung-Hoon , SHIN, Mi-Kyung , YOON, Sung-Sook , CHUN, Chang-Soo
IPC分类号： C12Q1/68
摘要： The invention relates to techniques for the rapid screening of suitable translational fusion partners (TFPs) capable of inducing secretory production of recombinant proteins, especially proteins that are difficult to produce using conventional recombinant production methods.

3. 发明申请

WO2012128577A3 METHOD FOR PERFORMANCE ANALYSIS AND SCREENING OF SEPTICAEMIA PROTEIN THERAPEUTIC AGENTS 审中-公开
标题翻译：分离蛋白质治疗药物的性能分析和筛选方法
公开(公告)号：WO2012128577A3
公开(公告)日：2013-01-24
申请号：PCT/KR2012002091
申请日：2012-03-22
申请人： KOREA RES INST OF BIOSCIENCE , KOREA ADVANCED INST SCI & TECH , LEE SEUNG GOO , KIM HAK SUNG , KIM YU JUNG , LEE SANG CHUL , HAN JI EUN , KIM SU JIN , SOHN JUNG HOON , HA JAE SEOK
发明人： LEE SEUNG GOO , KIM HAK SUNG , KIM YU JUNG , LEE SANG CHUL , HAN JI EUN , KIM SU JIN , SOHN JUNG HOON , HA JAE SEOK
IPC分类号： C12Q1/02 , C12N15/63 , G01N33/15 , G01N33/53 , G01N33/68
CPC分类号： C07K14/195 , C07K14/46 , C07K14/461 , C07K14/705 , C07K2319/35 , C07K2319/70 , C12N15/70 , C12Q1/6897 , G01N33/5023 , G06F19/16
摘要： The present invention relates to the construction of an assay system for analysing the performance of LRR (leucine rich repeat) proteins, that are constituted by the repeat combination of LRR modules, as septicaemia-inhibiting protein therapeutic agents, and for screening protein therapeutic agents that exhibit an outstanding inhibiting performance from a sequence variation library. More specifically, the present invention relates to: a performance analysis method or screening method for septicaemia protein therapeutic agents, comprising the step of adding purified MD-2 protein to an assay system using a cell line in which an LPS signal transmission receptor is expressed on the surface; a performance analysis method or screening method for septicaemia protein therapeutic agents, comprising the step of adding a purified foreign MD-2 protein to a THP-1 cell line; the cell line for performance analysis or for screening; and a kit for septicaemia therapeutic agent screening or performance analysis, comprising the cell line and purified foreign MD-2.
摘要翻译：本发明涉及用于分析LRR（富含亮氨酸重复）蛋白质的性能的测定系统的构建，其由LRR模块的重复组合构成，作为败血病抑制蛋白质治疗剂，并用于筛选蛋白质治疗剂，从序列变异库显示出优异的抑制性能。更具体地，本发明涉及：败血病蛋白治疗剂的性能分析方法或筛选方法，包括将纯化的MD-2蛋白加入到使用其中表达LPS信号传递受体的细胞系的测定系统的步骤表面用于败血病蛋白治疗剂的性能分析方法或筛选方法，包括将纯化的外源MD-2蛋白加入到THP-1细胞系中的步骤; 用于性能分析或筛选的细胞系; 以及用于败血症治疗剂筛选或性能分析的试剂盒，其包含细胞系和纯化的外源MD-2。

4. 发明申请

WO2012141547A3 ENHANCED-ACTIVITY MODIFIED BETA-GLUCOSIDASE AND A MANUFACTURING METHOD FOR BIOETHANOL USING SAME 审中-公开
标题翻译：增强活性改性的β-葡糖苷酶和使用其的生物柴油的制造方法
公开(公告)号：WO2012141547A3
公开(公告)日：2013-01-10
申请号：PCT/KR2012002846
申请日：2012-04-13
申请人： KOREA RES INST OF BIOSCIENCE , SOHN JUNG HOON , KIM HYUN JIN , KIM CHUL HO , SEOMOON JI HUN , SUNG BONG HYUN , LIM KWANG MOOK , KIM MI JIN , KIM JONG HYUN
发明人： SOHN JUNG HOON , KIM HYUN JIN , KIM CHUL HO , SEOMOON JI HUN , SUNG BONG HYUN , LIM KWANG MOOK , KIM MI JIN , KIM JONG HYUN
IPC分类号： C12N15/56 , C12N9/24 , C12N15/31 , C12P7/06
CPC分类号： C12P7/06 , C12N9/2445 , C12Y302/01021 , Y02E50/17
摘要： The present invention relates to a beta-glucosidase which has been modified so as to enhance the activity thereof, and to a production method for bioethanol using same. More specifically, the present invention relates to: a polynucleotide coding for a beta-glucosidase which has been modified so as to enhance the activity thereof; beta-glucosidase expressed from the polynucleotide; an expression vector comprising the polynucleotide; a transformant genetically transformed by means of the expression vector; a method for producing the modified beta-glucosidase by using the transformant; and a method for producing bioethanol by using the transformant. The modified beta-glucosidase of the present invention can be used to implement a saccharification process which is even more efficient than with existing beta-glucosidase, and can therefore be widely put to use in the economic production of bioethanol.
摘要翻译：本发明涉及已经被改性以提高其活性的β-葡糖苷酶以及使用它的生物乙醇的生产方法。更具体地，本发明涉及：已经修饰以增强其活性的β-葡糖苷酶编码的多核苷酸; 由多核苷酸表达的β-葡糖苷酶; 包含该多核苷酸的表达载体; 通过表达载体进行遗传转化的转化体; 通过使用转化体生产修饰的β-葡糖苷酶的方法; 以及使用该转化体生产生物乙醇的方法。本发明的经修饰的β-葡糖苷酶可用于实现比现有β-葡糖苷酶更有效的糖化方法，因此可广泛用于经济生产生物乙醇。

5. 发明申请

WO2012060666A3 METHOD FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR IN LARGE VOLUME FROM YEAST 审中-公开
标题翻译：从日本大批量生产人类生殖生长因子的方法
公开(公告)号：WO2012060666A3
公开(公告)日：2012-07-26
申请号：PCT/KR2011008390
申请日：2011-11-04
申请人： KOREA RES INST OF BIOSCIENCE , SOHN JUNG HOON , BEA JUNG HOON , KIM MI JIN , KIM HYUN JIN , PARK SOON HO , LIM KWANG MOOK
发明人： SOHN JUNG HOON , BEA JUNG HOON , KIM MI JIN , KIM HYUN JIN , PARK SOON HO , LIM KWANG MOOK
IPC分类号： C12N15/81 , C07K14/485 , C12N1/19 , C12N15/12
CPC分类号： C07K14/485 , C12N15/81 , C12R1/645
摘要： The present invention relates to a method for producing hEGF(human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
摘要翻译：本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

6. 发明申请

WO2004024954A1 METHOD FOR SCREENING OF A LIPASE HAVING IMPROVED ENZYMATIC ACTIVITY USING YEAST SURFACE DISPLAY VECTOR AND THE LIPASE 审中-公开
标题翻译：使用YEAST表面显示向量和脂质体筛选具有改善的酶活性的脂肪酶的方法
公开(公告)号：WO2004024954A1
公开(公告)日：2004-03-25
申请号：PCT/KR2003/001820
申请日：2003-09-04
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHOI, Eui-Sung , SOHN, Jung-Hoon , KIM, So-Young
发明人： CHOI, Eui-Sung , SOHN, Jung-Hoon , KIM, So-Young
IPC分类号： C12Q1/68
CPC分类号： C40B40/02 , C12N9/18 , C12N15/1037
摘要： The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry.
摘要翻译：本发明涉及使用酵母表面显示载体筛选具有改善的酶活性的脂肪酶的方法和由其制备的突变脂肪酶，更具体地涉及包括以下的方法： 1）将脂肪酶基因克隆到表面展示载体中，2）通过诱变PCR制备步骤1的突变脂肪酶基因文库，3）将步骤2的突变体脂肪酶基因文库和表面显示载体转化到宿主细胞中，4）测量突变脂肪酶的活性显示在转化的宿主细胞的表面，并选择由其制备的突变体脂肪酶。本发明的方法可以筛选具有改善的酶活性的脂肪酶。因此，它可以有效地用于食品和洗涤剂行业等各个领域。

7. 发明申请

WO2011004994A2 A NOVEL GENOME WALKING METHOD FOR CLONING OF UNKNOWN DNA SEQUENCES ADJACENT TO KNOWN SEQUENCE 审中-公开
标题翻译：克隆未知DNA序列与已知序列相邻的一种新的基因组步骤
公开(公告)号：WO2011004994A2
公开(公告)日：2011-01-13
申请号：PCT/KR2010004365
申请日：2010-07-06
申请人： KOREA RES INST OF BIOSCIENCE , SOHN JUNG-HOON , BAE JUNG-HOON
发明人： SOHN JUNG-HOON , BAE JUNG-HOON
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/11
CPC分类号： C12Q1/6855 , C12Q2525/186
摘要： The present invention relates to a novel genome walking method for cloning unknown genomic regions adjacent to known sequences by PCR. More particularly, the present invention relates to a method for cloning an unknown nucleotide sequence adjacent to a known nucleotide sequence, comprising the steps of 1) cleaving a genomic DNA that contains a known nucleotide sequence and an unknown nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3'-OH end of the cleaved DNA with a nucleotide analogue; 3) linking the terminal sequence of the cleaved DNA fragment with a linker, adapter or cassette having a terminal sequence complementary thereto; and 4) amplifying the unknown nucleotide sequence using a known nucleotide sequence-specific primer and a cassette sequence-specific primer. Further, the present invention relates to a composition of gene amplification for cloning the unknown nucleotide sequence, and a gene amplification kit comprising the composition.
摘要翻译：本发明涉及用于通过PCR克隆与已知序列相邻的未知基因组区域的新型基因组步行方法。更具体地说，本发明涉及克隆与已知核苷酸序列相邻的未知核苷酸序列的方法，该方法包括以下步骤：1）用限制性酶切割含有已知核苷酸序列和与其相邻的未知核苷酸序列的基因组DNA ; 2）用核苷酸类似物修饰切割的DNA的3'-OH末端; 3）将切割的DNA片段的末端序列与具有与其互补的末端序列的接头，接头或盒连接; 和4）使用已知的核苷酸序列特异性引物和盒序列特异性引物扩增未知的核苷酸序列。此外，本发明涉及用于克隆该未知核苷酸序列的基因扩增组合物，以及包含该组合物的基因扩增试剂盒。

8. 发明申请

WO2010064748A1 SCREENING OF ABUNDANTLY SECRETED PROTEINS AND THEIR USE AS FUSION PARTNERS FOR THE PRODUCTION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：过度分泌蛋白质的筛选及其作为重组蛋白生产的融合合作伙伴使用
公开(公告)号：WO2010064748A1
公开(公告)日：2010-06-10
申请号：PCT/KR2008/007231
申请日：2008-12-05
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , SOHN, Jung-Hoon , BAE, Jung-Hoon , KIM, Hyun-Jin , LIM, Kwang-Mook , KIM, Seung Il
发明人： SOHN, Jung-Hoon , BAE, Jung-Hoon , KIM, Hyun-Jin , LIM, Kwang-Mook , KIM, Seung Il
IPC分类号： C07K19/00 , C07K14/435 , C07K14/00 , C12N15/09 , C12N15/12 , C12N15/62
CPC分类号： C12N15/625 , C07K2319/02 , C12N15/1051 , C12P21/02
摘要： The present invention relates techniques for identifying suitable secretion fusion partner (SFP) for hyper-secretory production of recombinant proteins. The SFPs can be obtained from secretome analyses. Recombinant proteins are produced in a fusion form with a secretion fusion partner (SFP) and can be separated from the SFP by in vitro protease treatment. SFPs of this invention greatly improve the secretion level of target proteins and peptides which are valuable for bio-pharmaceuticals and the bio-industry.
摘要翻译：本发明涉及用于鉴定用于重组蛋白质的超分泌生产的合适的分泌融合伴侣（SFP）的技术。 SFP可以从分泌物分析中获得。以分泌融合配偶体（SFP）的融合形式产生重组蛋白质，并可通过体外蛋白酶处理从SFP分离。本发明的SFP大大提高了对生物制药和生物工业有价值的靶蛋白和肽的分泌水平。

9. 发明申请

WO2007015178A2 LIBRARY OF TRANSLATIONAL FUSION PARTNERS FOR PRODUCING RECOMBINANT PROTEINS AND TRANSLATIONAL FUSION PARTNERS SCREENED THEREFROM 审中-公开
标题翻译：用于生产重组蛋白和翻译融合合作伙伴的翻译融合合作伙伴图书馆
公开(公告)号：WO2007015178A2
公开(公告)日：2007-02-08
申请号：PCT/IB2006003102
申请日：2006-07-13
申请人： KOREA RES INST OF BIOSCIENCE , SOHN JUNG-HOON , CHOI EUI-SUNG , BAE JUNG-HOON , SHIN MI-KYUNG , YOON SUNG-SOOK , CHUN CHANG-SOO
发明人： SOHN JUNG-HOON , CHOI EUI-SUNG , BAE JUNG-HOON , SHIN MI-KYUNG , YOON SUNG-SOOK , CHUN CHANG-SOO
CPC分类号： C12P21/02 , C07K14/39 , C12N15/1086 , C12N15/81
摘要： The invention relates to techniques for the rapid screening of suitable translational fusion partners (TFPs) capable of inducing secretory production of recombinant proteins, especially proteins that are difficult to produce using conventional recombinant production methods.
摘要翻译：本发明涉及用于快速筛选能够诱导重组蛋白分泌生产的合适的翻译融合伴侣（TFP）的技术，特别是使用常规重组生产方法难以产生的蛋白质。

10. 发明申请

WO2005068658A1 RAPID SCREENING METHOD OF TRANSLATIONAL FUSION PARTNERS FOR PRODUCING RECOMBINANT PROTEINS AND TRANSLATIONAL FUSION PARTNERS SCREENED THEREFROM 审中-公开
标题翻译：用于生产重组蛋白的翻译融合合作伙伴的快速筛选方法和筛选的翻译融合合作伙伴
公开(公告)号：WO2005068658A1
公开(公告)日：2005-07-28
申请号：PCT/KR2004/003517
申请日：2004-12-30
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , SOHN, Jung-Hoon , CHOI, Eui-Sung , BAE, Jung-Hoon , LEE, Eung-Suck , SHIN, Mi-Kyung
发明人： SOHN, Jung-Hoon , CHOI, Eui-Sung , BAE, Jung-Hoon , LEE, Eung-Suck , SHIN, Mi-Kyung
IPC分类号： C12Q1/68
CPC分类号： C12P21/02 , C07K14/535 , C07K14/55 , C07K2319/00 , C07K2319/35 , C07K2319/61 , C12N9/20 , C12N15/1086
摘要： Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.
摘要翻译：公开了一种快速筛选能够诱导来自多种遗传来源的常规重组生产方法难以产生的非生产性蛋白质的表达或分泌生产的合适的翻译融合伴侣（TFP）的方法，以及蛋白质分泌 - 诱导使用该方法获得的TFP。

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