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    • 2. 发明申请
    • ENZYMATIC OIL-DEGUMMING METHOD
    • ENIYYY油脂脱水方法
    • WO2006008508A1
    • 2006-01-26
    • PCT/GB2005/002823
    • 2005-07-18
    • DANISCO A/SSOE, Jorn, BorchTURNER, Mark
    • SOE, Jorn, BorchTURNER, Mark
    • C11B3/00
    • C12N9/1029A21D8/042A23J7/00C11B3/003C12N9/20C12P7/6418C12P7/6445C12P7/6481C12Y301/01003
    • A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
    • 一种酶促脱脂食用油的方法,包括用脂质酰基转移酶处理食用油,以将酰基从大部分磷脂转移到一个或多个酰基受体上,其中酰基受体可以是包含羟基的任何化合物 。 在一个实施方案中,优选地,酰基受体是水,并且在另一个实施方案中,优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时,产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含以下一个或多个氨基酸序列:SEQ ID No.1,SEQ ID No.3,SEQ ID No.4,SEQ ID No.5,SEQ ID No. 6号,SEQ ID No.7,SEQ ID No.8,SEQ ID No.9,SEQ ID No.10,SEQ ID No.11,SEQ ID No.12,SEQ ID No.13,SEQ ID No. 14,或SEQ ID No.15,SEQ ID No.16,SEQ ID No.17,SEQ ID No.18,SEQ ID No.36,SEQ ID No.38,SEQ ID No.40,SEQ ID No.41 ,SEQ ID No.45,SEQ ID No.47,SEQ ID No.50或与其同一性为75%以上的氨基酸序列。 还教导了包含SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。
    • 5. 发明申请
    • PROCESS
    • 处理
    • WO2009081094A2
    • 2009-07-02
    • PCT/GB2008004064
    • 2008-12-11
    • DANISCOSOE JORN BORCHBROWN ANNE VICTORIA
    • SOE JORN BORCHBROWN ANNE VICTORIA
    • C11B3/00C12N9/10C12N9/20C12P7/64
    • C12P7/6445C11B3/003C12Y203/01043C12Y301/04003
    • A process of water degumming an edible oil (preferably a crude edible oil) comprising the steps of: a) admixing approximately 0.1-5% w/w water with an edible oil (preferably a crude edible oil) and a lipid acyltransferase, b) agitating the admixture for between about 10 minutes and 180 minutes at about 45 to about 900C, and c) separating the oil phase and the gum phase. Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith. In one embodiment the lipid acyltransferase is preferably used in combination with a phospholipase C enzyme. A process for modifying the gum phase of a degummed oil using a lipid acyltransferase is also taught herein.
    • 一种将食用油(优选粗制食用油)脱水的方法,包括以下步骤:a)将约0.1-5%w / w的水与食用油(优选粗制食用油)和脂质酰基转移酶混合,b) 在约45至约900℃下搅拌混合物约10分钟至180分钟,以及c)分离油相和胶相。 优选地,所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽,其多肽通过表达如SEQ ID No.49所示的核苷酸序列或与其同一性具有70%或更多同一性的核苷酸序列获得; 和/或通过在中等严格条件下与包含SEQ ID No.49所示的核苷酸序列的核酸探针杂交的核酸的表达获得; 和/或是具有脂质酰基转移酶活性的多肽,该多肽包含SEQ ID No.68所示的氨基酸序列或与其同一性具有70%以上的氨基酸序列。 在一个实施方案中,脂质酰基转移酶优选与磷脂酶C酶组合使用。 本文还教导了使用脂质酰基转移酶改性脱胶油的胶相的方法。