专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2003055906A1 MODIFIED BACTERIAL SURFACE LAYER PROTEINS 审中-公开
标题翻译：改性细菌表面层蛋白
公开(公告)号：WO2003055906A1
公开(公告)日：2003-07-10
申请号：PCT/EP2002/014749
申请日：2002-12-23
申请人： NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO , POUWELS, Pieter, Hendrik , SMIT, Egbert , TIELEN, Frans
发明人： POUWELS, Pieter, Hendrik , SMIT, Egbert , TIELEN, Frans
IPC分类号： C07K14/335
CPC分类号： C07K14/335 , C07K2319/00 , C12N2810/55
摘要： Modified bacterial surface layer (S-layer) proteins are disclosed where the modification is the insertion, at an internal location, of a heterologous polypeptide, or polypeptide of interest. The polypeptide is a binding or target protein, such as an antigen or antibody, or part thereof, in particular a bacterial antigen (e.g. from Clostridium tetani such as TTFC). The modified surface layer protein can then be expressed on the surface of the bacterial cell and used in a vaccine. Also disclosed are bacteria which have been modified to express a heterologous surface layer protein, but which do not as a wild-type possess an S-layer (such as L. casei), and modified bacteria which express only a modified surface layer protein (and not the wild-type S-layer protein). The wild type S-layer is completely replaced with the modified version where the polynucleotide encoding the modified version is integrated into the bacterial genome. The modified S-proteins can form crystalline arrays, sheets or layers that can be used to bind functional molecules (e.g. receptors) to solid surfaces (Au, silicon wafers) in biosensors.
摘要翻译：公开了修饰的细菌表面层（S层）蛋白，其中修饰是在异源多肽或感兴趣的多肽的内部位置插入。多肽是结合或靶蛋白，例如抗原或抗体，或其部分，特别是细菌抗原（例如来自破伤风梭菌如TTFC）。然后可以将修饰的表面蛋白质在细菌细胞的表面上表达并用于疫苗中。还公开了已经被修饰以表达异源表面蛋白但是不具有野生型的具有S层（例如干酪乳杆菌）的细菌和仅表达改性表面蛋白的修饰细菌（而不是野生型S层蛋白）。野生型S层完全被编码修饰版本的多核苷酸整合到细菌基因组中的修饰版本替代。修饰的S-蛋白质可以形成可用于将功能性分子（例如受体）结合到生物传感器中的固体表面（Au，硅晶片）的晶体阵列，片或层。

IPRDB

热门服务

关于我们

友情链接

联系方式