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    • 2. 发明申请
    • METHODS FOR DETECTING TARGETS
    • 检测目标的方法
    • WO2006031663A3
    • 2006-05-11
    • PCT/US2005032257
    • 2005-09-09
    • GENENCOR INTSHERWOOD STEVEN W
    • SHERWOOD STEVEN W
    • C12Q1/34G01N33/58
    • G01N33/56966G01N33/57492
    • The present invention provides methods and compositions for detecting target molecules present on cells and tissues. In particular, the methods involve adding primary antibodies such as scFv-targeted lactamase that are directed against a target of interest (e.g., cancer markers) to a tissue sample, followed by adding a lactam-containing compound and finally a lactamase reporter system. In some preferred embodiments, the lactamase reporters are fluorescent reporters that bind to the test tissue. In some particularly preferred embodiments, the test tissue contains at least one cancer cell and/or at least one cancer-associated marker.
    • 本发明提供用于检测存在于细胞和组织上的靶分子的方法和组合物。 特别地,所述方法包括将针对感兴趣靶标(例如癌症标志物)的一级抗体(例如scFv靶向的内酰胺酶)加入到组织样品中,随后加入含内酰胺的化合物,最后加入内酰胺酶报告系统。 在一些优选的实施方案中,内酰胺酶报告物是与测试组织结合的荧光报告物。 在一些特别优选的实施方案中,测试组织含有至少一种癌细胞和/或至少一种癌相关标志物。