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    • 2. 发明申请
    • A SOLID STATE MATRIX, PROCESS OF PREPARATION THEREOF, AND PROCESS OF PREPARATION OF THEAFLAVINS
    • 固体状态矩阵,其制备方法及其制备方法
    • WO2007116259A2
    • 2007-10-18
    • PCT/IB2007000699
    • 2007-03-21
    • COUNCIL SCIENT IND RESSINGH HARSH PRATAPSHARMA KAPIL
    • SINGH HARSH PRATAPSHARMA KAPIL
    • C08F220/18C08F8/30C12N11/08
    • C08F220/18C08F8/30C12N11/08C12P17/06C08F20/00
    • The present invention relates to a process for the development of a highly efficient solid state matrix by the activation of acrylate based polymer resin having specialized functional groups with 1,1-Carbonyl diimidazole for immobilizing biologically active macromolecules such as oxidases, in particular plant oxidases and the most preferred being tea polyphenol oxidase through indirect covalent bonding/cross linking on such activated polymer resin support, are thermally stable, gives very high number of turnovers in vitro ("n" times) with tea substrate forming exclusive product Theaflavins without any loss of biological activity and leaving the product remaining in vitro without adherence to matrix rendering the matrix safe towards product poisoning and subsequent partial or complete loss of biological activity of the matrix bound enzyme system and thus well adapted to and well suited bioreactors based on such systems. It is unique with respect to its recyclability of otherwise uneconomical tea substrates such as seed and flower substrates into theaflavins both with respect to crude substrate or purified ones.
    • 本发明涉及通过活化具有特殊官能团的具有1,1-羰基二咪唑的丙烯酸酯类聚合物树脂来固定生物活性大分子如氧化酶,特别是植物氧化酶,开发高效固态基质的方法,以及 最优选的是茶多酚氧化酶通过间接共价键/交联在这种活化的聚合物树脂载体上,是热稳定的,在茶叶底物形成独特产品时,在体外(“n”倍)时产生非常高数量的周转量茶黄素没有任何损失 生物活性并使产物保留在体外,而不依赖于基质,使得基质对产品中毒和随后的基质结合酶系统的生物活性的部分或完全丧失是安全的,因此很好地适应于基于这种系统的和非常适合的生物反应器。 对于其他不经济的茶基质,如对于粗制底物或纯化的茶碱,其对茶黄素的种子和花基质的再循环性是独一无二的。