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1. 发明专利

DE69330162D1 未知
公开(公告)号：DE69330162D1
公开(公告)日：2001-05-23
申请号：DE69330162
申请日：1993-09-22
申请人： KANAGAWA KAGAKU GIJUTSU AKAD , KATO SEISHI , SEKINE SHINGO
发明人： KATO SEISHI , SEKINE SHINGO
IPC分类号： C12N15/09 , C12N15/10 , C12N15/66 , C07H21/02 , C07H21/04
摘要： The invention discloses a method of providing a cDNA containing the whole of the information on the primary structure of a protein by selectively synthesizing only the complete-length cDNA that contains a sequence beginning with the cap site of a mRNA. The process for producing an intermediate for the synthesis of the complete-length cDNA comprises the step of treating a mRNA extracted from cells with an alkaline phosphatase to eliminate the phosphate group from the 5' end of an uncapped incomplete-length mRNA, the step of decapping from the 5' end of a capped complete-length mRNA, and the step of linking the 5'-end phosphate group formed in the above step to either the DNA oligonucleotide represented by the following general formula (I) or a DNA-RNA chimera oligonucleotide by means of a T4RNA ligase to selectively add the DNA oligonucleotide or DNA-RNA chimera oligonucleotide having an arbitrary sequence to the 5' end of the complete-length mRNA: 5'-dN1-dN2- -dNm-N1N2- -Nn-3', wherein dN represents a deoxyribonucleotide selected from among dAMP, dCMP, dGMP and dTMP; N represents a ribonucleotide selected from among AMP, CMP, GMP and UMP; "-" represents a phosphoric ester linkage; m represents an integer of 1 or above; and n represents an integer of 0 or above. The process for synthesizing a complete-length cDNA comprises linking a double-stranded DNA primer having a dT tail by annealing to the poly (A) tail of the 3' end of the complete-length mRNA having the 5' end to which the DNA oligonucleotide or DNA-RNA chimera oligonucleotide have been added as described above, and then synthesizing a single-stranded cDNA complementary to the complete-length mRNA by means of a reverse transcriptase.

2. 发明公开

EP0625572A1 PROCESS FOR SYNTHESIZING COMPLETE-LENGTH cDNA, PROCESS FOR PRODUCING INTERMEDIATE THEREFOR, AND PROCESS FOR PRODUCING RECOMBINANT VECTOR CONTAINING COMPLETE-LENGTH cDNA 失效
标题翻译： METHOD FOR IN合成全长cDNA PROCESS FOR PRODUCING中间体和用于制备重组载体使用完整cDNA含有。
公开(公告)号：EP0625572A1
公开(公告)日：1994-11-23
申请号：EP93921061.3
申请日：1993-09-22
申请人： THE KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY FOUNDATION , KATO, Seishi , SEKINE, Shingo
发明人： KATO, Seishi 1-9-2-304, Minamidai , SEKINE, Shingo 4-4-1, Nishioonuma
IPC分类号： C12N15/10
CPC分类号： C12N15/1096 , C12N15/66
摘要： The invention discloses a method of providing a cDNA containing the whole of the information on the primary structure of a protein by selectively synthesizing only the complete-length cDNA that contains a sequence beginning with the cap site of a mRNA. The process for producing an intermediate for the synthesis of the complete-length cDNA comprises the step of treating a mRNA extracted from cells with an alkaline phosphatase to eliminate the phosphate group from the 5' end of an uncapped incomplete-length mRNA, the step of decapping from the 5' end of a capped complete-length mRNA, and the step of linking the 5'-end phosphate group formed in the above step to either the DNA oligonucleotide represented by the following general formula (I) or a DNA-RNA chimera oligonucleotide by means of a T4RNA ligase to selectively add the DNA oligonucleotide or DNA-RNA chimera oligonucleotide having an arbitrary sequence to the 5' end of the complete-length mRNA: 5'-dN₁-dN₂- ... -dN m -N₁N₂- ... -N n -3', wherein dN represents a deoxyribonucleotide selected from among dAMP, dCMP, dGMP and dTMP; N represents a ribonucleotide selected from among AMP, CMP, GMP and UMP; "-" represents a phosphoric ester linkage; m represents an integer of 1 or above; and n represents an integer of 0 or above. The process for synthesizing a complete-length cDNA comprises linking a double-stranded DNA primer having a dT tail by annealing to the poly (A) tail of the 3' end of the complete-length mRNA having the 5' end to which the DNA oligonucleotide or DNA-RNA chimera oligonucleotide have been added as described above, and then synthesizing a single-stranded cDNA complementary to the complete-length mRNA by means of a reverse transcriptase.
摘要翻译：本发明盘松动提供的cDNA通过选择性地仅合成完整的全长cDNA确实包含与mRNA的帽位点开始的序列包含整个的上的蛋白质的一级结构中的信息的方法。在中间的完整长度cDNA的合成的制造方法包括：在碱性磷酸酯酶从细胞治疗的mRNA萃取从一个未加帽的不完全长度的mRNA的5“末端的步骤，消除磷酸基团的步骤从封端的完整长度的mRNA的5“端，并在上述步骤或者由下述通式（I）表示的DNA寡核苷酸所形成的5'-末端磷酸基团连接的步骤或DNA-RNA脱帽嵌合体寡核苷酸由T4RNA连接酶的装置以选择性地添加到具有任意序列的完整长度的mRNA的5“末端的DNA寡核苷酸或DNA-RNA嵌合体寡核苷酸：5'-DN1-DN2 - <...> - DNM -N1N2 - <...> - NN-3”，worin dN的darstellt选自潮湿，的dCMP，和的dGMP选择的dTMP脱氧核糖核苷酸; Ñdarstellt从AMP，CMP，GMP和UMP中选择的核糖核苷酸; “ - ” darstellt磷酸酯键; 上的1或以上的整数米darstellt; 和n darstellt为0或以上的整数。用于合成完整的全长cDNA包括连接具有由退火的dT尾到尾端，与DNA的3“具有5的完整长度的mRNA的末端”的多聚（A）尾的双链DNA引物的方法如上所述，然后合成的单链cDNA互补利用逆转录酶的指完整长度的mRNA已被添加寡核苷酸或DNA-RNA嵌合体寡核苷酸。

3. 发明公开

EP0625572A4 PROCESS FOR SYNTHESIZING COMPLETE-LENGTH cDNA, PROCESS FOR PRODUCING INTERMEDIATE THEREFOR, AND PROCESS FOR PRODUCING RECOMBINANT VECTOR CONTAINING COMPLETE-LENGTH cDNA. 失效
标题翻译： METHOD FOR IN合成全长cDNA PROCESS FOR PRODUCING中间体和用于制备重组载体使用完整cDNA含有。
公开(公告)号：EP0625572A4
公开(公告)日：1997-03-12
申请号：EP93921061
申请日：1993-09-22
申请人： KANAGAWA KAGAKU GIJUTSU AKAD , KATO SEISHI , SEKINE SHINGO
发明人： KATO SEISHI , SEKINE SHINGO
IPC分类号： C12N15/09 , C12N15/10 , C12N15/66
CPC分类号： C12N15/1096 , C12N15/66
摘要： The invention discloses a method of providing a cDNA containing the whole of the information on the primary structure of a protein by selectively synthesizing only the complete-length cDNA that contains a sequence beginning with the cap site of a mRNA. The process for producing an intermediate for the synthesis of the complete-length cDNA comprises the step of treating a mRNA extracted from cells with an alkaline phosphatase to eliminate the phosphate group from the 5' end of an uncapped incomplete-length mRNA, the step of decapping from the 5' end of a capped complete-length mRNA, and the step of linking the 5'-end phosphate group formed in the above step to either the DNA oligonucleotide represented by the following general formula (I) or a DNA-RNA chimera oligonucleotide by means of a T4RNA ligase to selectively add the DNA oligonucleotide or DNA-RNA chimera oligonucleotide having an arbitrary sequence to the 5' end of the complete-length mRNA: 5'-dN1-dN2- (* f HANDWRITTEN *) -dNm-N1-N2- (* f HANDWRITTEN *) -Nn-3', wherein dN represents a deoxyribonucleotide selected from among dAMP, dCMP, dGMP and dTMP; N represents a ribonucleotide selected from among AMP, CMP, GMP and UMP; "-" represents a phosphoric ester linkage; m represents an integer of 1 or above; and n represents an integer of 0 or above. The process for synthesizing a complete-length cDNA comprises linking a double-stranded DNA primer having a dT tail by annealing to the poly (A) tail of the 3' end of the complete-length mRNA having the 5' end to which the DNA oligonucleotide or DNA-RNA chimera oligonucleotide have been added as described above, and then synthesizing a single-stranded cDNA complementary to the complete-length mRNA by means of a reverse transcriptase.

4. 发明授权

EP0625572B1 PROCESS FOR SYNTHESIZING COMPLETE-LENGTH cDNA, PROCESS FOR PRODUCING INTERMEDIATE THEREFORE, AND PROCESS FOR PRODUCING RECOMBINANT VECTOR CONTAINING COMPLETE-LENGTH cDNA 失效
标题翻译： METHOD FOR IN合成全长cDNA PROCESS FOR PRODUCING中间体和用于制备重组载体使用完全cDNA含有
公开(公告)号：EP0625572B1
公开(公告)日：2001-04-18
申请号：EP93921061.3
申请日：1993-09-22
申请人： THE KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY FOUNDATION , KATO, Seishi , SEKINE, Shingo
发明人： KATO, Seishi 1-9-2-304, Minamidai , SEKINE, Shingo 4-4-1, Nishioonuma
IPC分类号： C12N15/10 , C12N15/66 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1096 , C12N15/66

5. 发明申请

WO9927094A3 HUMAN PROTEINS HAVING TRANSMEMBRANE DOMAINS AND DNAs ENCODING THESE PROTEINS 审中-公开
标题翻译：具有跨膜结构域的人蛋白质和编码这些蛋白质的DNA
公开(公告)号：WO9927094A3
公开(公告)日：1999-08-26
申请号：PCT/JP9805238
申请日：1998-11-20
申请人： SAGAMI CHEM RES , PROTEGENE INC , KATO SEISHI , KIMURA TOMOKO , SEKINE SHINGO
发明人： KATO SEISHI , KIMURA TOMOKO , SEKINE SHINGO
IPC分类号： C12N15/09 , A61K38/00 , A61K48/00 , A61P43/00 , C07K14/47 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/12 , C12N15/85
CPC分类号： C07K14/47
摘要： Human proteins having transmembrane domains, cDNAs coding for these proteins, and expression vectors of said cDNAs as well as eucaryotic cells expressing said cDNAs are provided. The proteins exist in the cell membrane and are considered to control the proliferation and the differentiation of the cells. The proteins can thus be employed as pharmaceuticals such as carcinostatic agents relating to the control of the proliferation and the differentiation of the cells or as antigens for preparing antibodies against said proteins. The cDNAs can be utilized as probes for the gene diagnosis and gene sources for the gene therapy. Furthermore, the cDNAs can be utilized for large-scale expression of said proteins. Cells, wherein these membrane protein genes are introduced and membrane proteins are expressed in large amounts, can be utilized for detection of the corresponding ligands, screening of novel low-molecular pharmaceuticals, and so on.
摘要翻译：提供了具有跨膜结构域的人蛋白质，编码这些蛋白质的cDNA，以及所述cDNA的表达载体以及表达所述cDNA的真核细胞。蛋白质存在于细胞膜中，并被认为控制细胞的增殖和分化。这些蛋白质因此可以用作药物，例如与控制细胞的增殖和分化有关的制癌剂，或用作制备针对所述蛋白质的抗体的抗原。这些cDNA可以用作基因诊断的探针和用于基因治疗的基因来源。此外，该cDNA可以用于所述蛋白质的大规模表达。其中引入这些膜蛋白基因并且大量表达膜蛋白的细胞可用于检测相应的配体，筛选新的低分子药物等。

6. 发明申请

WO9918203A3 HUMAN PROTEINS HAVING TRANSMEMBRANE DOMAINS AND cDNAs ENCODING THESE PROTEINS 审中-公开
标题翻译：具有跨膜结构域的人蛋白质和编码这些蛋白质的cDNA
公开(公告)号：WO9918203A3
公开(公告)日：1999-06-24
申请号：PCT/JP9804475
申请日：1998-10-05
申请人： SAGAMI CHEM RES , PROTEGENE INC , KATO SEISHI , KIMURA TOMOKO , SEKINE SHINGO , KOBAYASHI MIDORI
发明人： KATO SEISHI , KIMURA TOMOKO , SEKINE SHINGO , KOBAYASHI MIDORI
IPC分类号： C07K14/47 , C12N15/12 , C12N5/10 , C12N15/79
CPC分类号： C07K14/47
摘要： The invention provides human proteins having transmembrane domains and cDNAs coding for these proteins as well as eukaryotic cells expressing said cDNAs. All of the proteins exist in the cell membrane, so that they are considered to be proteins controlling the proliferation and the differentiation of the cells. Accordingly, the proteins can be employed as pharmaceuticals such as carcinostatic agents relating to the control of the proliferation and the differentiation of the cells or as antigens for preparing antibodies against said proteins. The cDNAs can be utilized as probes for the gene diagnosis and gene sources for the gene therapy. Furthermore, the cDNAs can be utilized for large-scale expression of said proteins. Cells, wherein these membrane protein genes are introduced and membrane proteins are expressed in large amounts, can be utilized for detection of the corresponding ligands, screening of novel low-molecular pharmaceuticals, and so on.
摘要翻译：本发明提供了具有跨膜结构域的人蛋白质和编码这些蛋白质的cDNA以及表达所述cDNA的真核细胞。所有的蛋白质都存在于细胞膜中，因此它们被认为是控制细胞增殖和分化的蛋白质。因此，蛋白质可以作为药物使用，例如涉及控制细胞增殖和分化的抗癌剂或作为抗所述蛋白质的抗体的抗原。这些cDNA可以用作基因诊断的探针和用于基因治疗的基因来源。此外，该cDNA可以用于所述蛋白质的大规模表达。其中引入这些膜蛋白基因并且大量表达膜蛋白的细胞可用于检测相应的配体，筛选新的低分子药物等。

7. 发明申请

WO9855508A3 HUMAN PROTEINS HAVING TRANSMEMBRANE DOMAINS AND DNAs ENCODING THESE PROTEINS 审中-公开
标题翻译：具有转录因子的人蛋白质和编码这些蛋白质的DNA
公开(公告)号：WO9855508A3
公开(公告)日：1999-03-25
申请号：PCT/JP9802445
申请日：1998-06-03
申请人： SAGAMI CHEM RES , PROTEGENE INC , KATO SEISHI , SEKINE SHINGO , YAMAGUCHI TOMOKO
发明人： KATO SEISHI , SEKINE SHINGO , YAMAGUCHI TOMOKO
IPC分类号： C12N15/09 , A61K38/00 , C07H21/04 , C07K14/705 , C12N1/15 , C12N1/19 , C12N5/10 , C12N9/72 , C12N15/12 , A61K38/17 , C12N15/85 , C12Q1/37
CPC分类号： C07K14/705 , A61K38/00 , C12N9/6456
摘要： Proteins comprising any of the amino acid sequences of SEQ ID NOS: 1 to 18 and DNAs encoding said proteins and comprising any of the nucelotide sequences of SEQ ID NOS: 19 to 36 are provided.
摘要翻译：提供了包含SEQ ID NO：1至18的任何氨基酸序列的蛋白质和编码所述蛋白质的DNA并且包含SEQ ID NO：19至36的任一个硝酸苷序列的蛋白质。

8. 发明申请

WO1998021328A3 HUMAN PROTEINS HAVING TRANSMEMBRANE DOMAINS AND DNAS ENCODING THESE PROTEINS 审中-公开
公开(公告)号：WO1998021328A3
公开(公告)日：1998-05-22
申请号：PCT/JP1997004056
申请日：1997-11-07
申请人： SAGAMI CHEMICAL RESEARCH CENTER , PROTEGENE INC. , KATO, Seishi , SEKINE, Shingo , YAMAGUCHI, Tomoko , KOBAYASHI, Midori
发明人： SAGAMI CHEMICAL RESEARCH CENTER , PROTEGENE INC.
IPC分类号： C12N15/12

9. 发明申请

WO1997003190A1 HUMAN GALECTIN-4-LIKE PROTEIN AND cDNA ENCODING THE SAME 审中-公开
标题翻译：人类GALECTIN-4型蛋白和编码其的cDNA
公开(公告)号：WO1997003190A1
公开(公告)日：1997-01-30
申请号：PCT/JP1996001899
申请日：1996-07-09
申请人： SAGAMI CHEMICAL RESEARCH CENTER , KATO, Seishi , YAMAGUCHI, Tomoko , SEKINE, Shingo , KAMATA, Kouju
发明人： SAGAMI CHEMICAL RESEARCH CENTER
IPC分类号： C12N15/12
CPC分类号： C07K14/4726 , A61K38/00
摘要： A galectin-4 (a lactose-binding protein)-like protein expressed specifically in the stomach and intestines and a human cDNA encoding the same. The protein is one containing the amino acid sequence represented by SEQ ID NO: 1 while the gene is a cDNA containing the base sequence represented by SEQ ID NO: 2. The protein which is the expression product of this cDNA has a lactose-binding activity and is applicable to drugs and research reagents.
摘要翻译：在胃和肠中特异表达的半乳凝素-4（乳糖结合蛋白）样蛋白质和编码其的人cDNA。该蛋白质是含有SEQ ID NO：1所示的氨基酸序列的蛋白质，该基因是含有SEQ ID NO：2所示的碱基序列的cDNA。作为该cDNA的表达产物的蛋白质具有乳糖结合活性适用于药物和研究试剂。

10. 发明申请

WO9943802A3 HUMAN PROTEINS HAVING TRANSMEMBRANE DOMAINS AND DNAs ENCODING THESE PROTEINS 审中-公开
标题翻译：具有跨膜结构域的人蛋白质和编码这些蛋白质的DNA
公开(公告)号：WO9943802A3
公开(公告)日：1999-12-29
申请号：PCT/JP9900875
申请日：1999-02-25
申请人： SAGAMI CHEM RES , PROTEGENE INC , KATO SEISHI , SEKINE SHINGO , KIMURA TOMOKO , NAKAMURA NOBUKO
发明人： KATO SEISHI , SEKINE SHINGO , KIMURA TOMOKO , NAKAMURA NOBUKO
IPC分类号： C12N15/09 , C07K14/705 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/12 , C12P21/02
CPC分类号： C07K14/705
摘要： Human proteins having transmembrane domains, cDNAs coding for these proteins, and expression vectors of said cDNAs as well as eucaryotic cells expressing said cDNAs. Said proteins and eucaryotic cells having said proteins on the membrane surface can be provided by expression of cDNAs coding for human proteins having transmembrane domains and of recombinants of these human cDNAs.
摘要翻译：具有跨膜结构域的人蛋白质，编码这些蛋白质的cDNA，以及所述cDNA的表达载体以及表达所述cDNA的真核细胞。在膜表面上具有所述蛋白质的所述蛋白质和真核细胞可以通过表达编码具有跨膜结构域的人蛋白质和这些人cDNA的重组体的cDNA来提供。

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