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1. 发明申请

WO1994006917A1 PRODUCTION OF DESIRED PROTEINS OR POLYPEPTIDES BY CULTURING A TRANSFORMED LACTIC ACID BACTERIUM 审中-公开
标题翻译：通过培养转化的酸性细菌生产所需的蛋白质或多肽
公开(公告)号：WO1994006917A1
公开(公告)日：1994-03-31
申请号：PCT/EP1993002558
申请日：1993-09-20
申请人： UNILEVER PLC , UNILEVER N.V. , SEEGERS, Jozef, Franciscus, M., L. , KIEWIET, Rense , VENEMA, Gerard , BRON, Sierd
发明人： UNILEVER PLC , UNILEVER N.V.
IPC分类号： C12N15/74
CPC分类号： A23K10/18 , A23L29/065 , A23Y2220/00 , C07K14/315 , C12N15/52 , C12N15/746
摘要： The invention provides a food-grade recombinant plasmid comprising (1) a replicon and DNA sequences derived from a lactic acid bacterium (LAB), required for stable maintenance in a LAB, preferably derived from plasmid pWVO2 from (L. lactis) subsp. (cremoris) Wg2, and (2) a structural gene encoding a desired protein or polypeptide or its precursor, not being an antibiotic resistance selection marker, and optionally (3) a food-grade selection marker, whereby said plasmid has a theta-replication mechanism and is stably maintained in the transformed LAB under non-selective conditions. Also provided is a LAB transformed by said recombinant plasmid and the use of such transformed LAB in a process for producing said protein or polypeptide. Said recombinant plasmid can also be used for modifying the metabolic pathway of a LAB, e.g. for producing diacetyl or a precursor thereof by a LAB. Also claimed are food and animal feed products obtained by incorporating therein said protein or polypeptide or precursor thereof produced by said transformed LAB.
摘要翻译：本发明提供了一种食品级重组质粒，其包含（1）来自乳酸杆菌（LAB）的复制子和DNA序列，其需要在LAB中稳定维持，优选来源于来自（乳酸乳球菌）亚种的质粒pWVO2。（cremoris）Wg2，和（2）编码期望的蛋白质或多肽或其前体的结构基因，不是抗生素抗性选择标记，以及任选的（3）食品级选择标记，其中所述质粒具有θ复制机制，并在非选择性条件下稳定地保持在转化的LAB中。还提供了由所述重组质粒转化的LAB和在产生所述蛋白质或多肽的方法中使用这种转化的LAB。所述重组质粒也可用于修饰LAB的代谢途径，例如，用于通过LAB生产二乙酰基或其前体。还要求保护的是通过在其中掺入由所述转化的LAB产生的所述蛋白质或多肽或其前体获得的食物和动物饲料产品。

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