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1. 发明申请

WO2010092186A1 CONVERSION OF UNIPOTENT GERMLINE STEM CELLS INTO PLURIPOTENT STEM CELLS WITHOUT EXOGENOUS FACTORS 审中-公开
标题翻译：将不明原代干细胞转化为没有异常因子的多发性干细胞
公开(公告)号：WO2010092186A1
公开(公告)日：2010-08-19
申请号：PCT/EP2010/051928
申请日：2010-02-16
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN e. V. , SCHÖLER, Hans Robert , KO, Kinarm
发明人： SCHÖLER, Hans Robert , KO, Kinarm
IPC分类号： C12N5/076
CPC分类号： C12N5/0611 , C12N2501/11 , C12N2501/115 , C12N2501/13 , C12N2501/235 , C12N2501/392
摘要： The present invention relates to a method of generating pluripotent stem cells comprising the steps: (a) plating murine germiine stem cells (GSCs) at a density of 2O1OOO cells or less per 1.9 cm 2 ; and (b) cuituring the germiine stem cells for at least 11 days without subculturing while maintaining said germiine stem cells in an undifferentiated state, wherein during or after step (b) the presence of markers characteristic of pluripotent stem cells is indicative of the generation of pluripotent stem celis. Also, the invention relates to a pluripotent stem cell obtainable by the method of the invention and a cell obtainable by differentiation of said pluripotent stem cell. Further, the invention relates to a method of generating a transgenic non-human animal and a transgenic non-human animal generated by said method. Finally, the invention envisions a composition comprising a pluripotent stem cell of the invention.
摘要翻译：本发明涉及一种产生多能干细胞的方法，包括以下步骤：（a）以1.9cm2的浓度以20万个细胞或更少的细胞电镀鼠胚胎干细胞（GSC）; 并且（b）将胚胎干细胞培养至少11天，而不传代培养，同时保持所述胚胎干细胞处于未分化状态，其中在步骤（b）期间或之后，多能干细胞特征性标志物的存在表明多能茎干此外，本发明涉及通过本发明的方法获得的多能干细胞和通过所述多能干细胞的分化获得的细胞。此外，本发明涉及产生由所述方法产生的转基因非人动物和转基因非人动物的方法。最后，本发明设想了包含本发明多能干细胞的组合物。

2. 发明申请

WO2009144008A1 GENERATION OF INDUCED PLURIPOTENT STEM (IPS) CELLS 审中-公开
标题翻译：诱导性脑脊液（IPS）细胞的产生
公开(公告)号：WO2009144008A1
公开(公告)日：2009-12-03
申请号：PCT/EP2009/003735
申请日：2009-05-26
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. , KIM, Jeong, Beom , ZAEHRES, Holm , SCHÖLER, Hans, Robert
发明人： KIM, Jeong, Beom , ZAEHRES, Holm , SCHÖLER, Hans, Robert
IPC分类号： C12N5/06
CPC分类号： C12N5/0696 , A61D19/04 , C12N15/85 , C12N15/8509 , C12N15/873 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2502/13 , C12N2506/08 , C12N2506/13 , C12N2510/00 , C12N2799/027 , G01N33/5073
摘要： The present invention relates to a method of generating an induced pluripotent stem (iPS) cell comprising the step of introducing into a target cell one or two coding sequences each giving rise upon transcription to a factor that contributes to the reprogramming of said target cell into an induced pluripotent stem cell and selected from Oct3/4 or a factor belonging to the Myc, KIf and Sox families of factors, wherein the target cell endogenously expresses at least the factors that are not encoded by the coding sequences to be introduced and selected from Oct3/4 or factors belonging to the Myc, KIf and Sox families of factors, and wherein the cell resulting from the introduction of the one or two coding sequences expresses the combination of factor Oct3/4 and at least one factor of each family of factors selected from the group of Myc, KIf and Sox. Furthermore, the present invention relates to an induced pluripotent stem cell generated by the method of the invention and a method of identifying a compound that contributes to the reprogramming of a target cell into an induced pluripotent stem cell. Also, a method of generating a transgenic non-human animal and a composition comprising an iPS cell generated by the method of the present invention for gene therapy, regenerative medicine, cell therapy or drug screening are envisaged.
摘要翻译：本发明涉及一种产生诱导多能干细胞（iPS）细胞的方法，该方法包括以下步骤：向靶细胞中引入一个或两个编码序列，每个编码序列在转录时产生有助于将所述靶细胞重编程为诱导的多能干细胞，选自Oct3 / 4或属于Myc，KIf和Sox家族因子的因子，其中靶细胞至少内源地表达至少不是由Oct3引入和选择的编码序列编码的因子 / 4或属于Myc，KIf和Sox家族因子的因子，并且其中由引入一个或两个编码序列产生的细胞表达因子Oct3 / 4与所选因子的至少一个因子的组合来自Myc，KIf和Sox的组。此外，本发明涉及通过本发明的方法产生的诱导多能干细胞和鉴定有助于靶细胞重编程为诱导多能干细胞的化合物的方法。此外，设想生成转基因非人动物的方法和包含通过本发明的方法产生的用于基因治疗，再生医学，细胞治疗或药物筛选产生的iPS细胞的组合物。

3. 发明申请

WO1989006698A1 PROCESS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：WO1989006698A1
公开(公告)日：1989-07-27
申请号：PCT/EP1989000026
申请日：1989-01-12
申请人： BOEHRINGER MANNHEIM GMBH , HÖLTKE, Hans-Joachim , SEIBL, Rudolf , SCHMITZ, Gudrun , SCHÖLER, Hans, Robert , KESSLER, Christop , MATTES, Ralf
发明人： BOEHRINGER MANNHEIM GMBH
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6804 , C12Q1/686 , C12Q2563/131 , C12Q2525/117
摘要： Nucleic acids of specific sequence are detected by hybridization with a complementary nucleic acid probe linked through a chemical bond with at least one label in the form of a hapten. The hapten is a steroid linked by a bridge at least 4 atoms long to at least one position of the nucleic acid probe which is not involved in the formation of the hydrogen bridge and detects the hybridized probe by means of an antihapten antibody which is also labelled.

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