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1. 发明申请

WO2005123937A3 METHODS FOR EXPRESSING RNP PARTICLES IN EUKARYOTIC CELLS 审中-公开
标题翻译：用于在核细胞中表达RNP颗粒的方法
公开(公告)号：WO2005123937A3
公开(公告)日：2006-04-13
申请号：PCT/US2005020893
申请日：2005-06-14
申请人： UNIV TEXAS AT AUSTIN , CUI XIAOXIA , LAMBOWITZ ALAN , SALDANHA ROLAND
发明人： CUI XIAOXIA , LAMBOWITZ ALAN , SALDANHA ROLAND
IPC分类号： C12N15/12 , C12N15/63 , C12N15/85 , C12P21/06 , C07H21/02 , C07H21/04 , C12P21/00
CPC分类号： C12N15/63 , C12N15/85
摘要： Provided herein are nucleic acid constructs and methods for producing or enhancing the production of group II intron RNP particles in eukaryotic cells. The present methods comprise introducing at least one nucleic acid construct comprising a nucleic acid encoding a modified or wild type group II intron RNA and a wild-type or modified group II intron-encoded protein into the eukaryotic cell, and maintaining the cell under conditions that allow for expression of the group II intron RNA and the group II intron-encoded protein in the cell. The nucleic acid encoding the group II intron RNA is operably linked to an RNA polymerase I, an RNA polymerase II, or an RNA polymerase III promoter, and the nucleic acid encoding the group II intron-encoded protein is operably linked to an RNA polymerase II promoter. In certain embodiments, a subcellular localization signal is attached to the group II intron-encoded protein.
摘要翻译：本文提供了核酸构建体和用于在真核细胞中产生或增强II组内含子RNP颗粒的生产的方法。本发明的方法包括将包含编码经修饰或野生型II组内含子RNA的核酸和野生型或修饰II型内含子编码蛋白质的至少一种核酸构建体引入真核细胞中，并将细胞维持在允许在细胞中表达II组内含子RNA和II组内含子编码的蛋白质。编码II组内含子RNA的核酸可操作地连接到RNA聚合酶I，RNA聚合酶II或RNA聚合酶III启动子，编码II组内含子编码蛋白的核酸可操作地连接到RNA聚合酶II 子。在某些实施方案中，将亚细胞定位信号连接到II组内含子编码的蛋白质。

2. 发明申请

WO2005123937A2 METHODS FOR EXPRESSING RNP PARTICLES IN EUKARYOTIC CELLS 审中-公开
标题翻译：用于在核细胞中表达RNP颗粒的方法
公开(公告)号：WO2005123937A2
公开(公告)日：2005-12-29
申请号：PCT/US2005/020893
申请日：2005-06-14
申请人： THE UNIVERSITY OF TEXAS AT AUSTIN , CUI, Xiaoxia , LAMBOWITZ, Alan , SALDANHA, Roland
发明人： CUI, Xiaoxia , LAMBOWITZ, Alan , SALDANHA, Roland
IPC分类号： C12P21/06
CPC分类号： C12N15/63 , C12N15/85
摘要： Provided herein are nucleic acid constructs and methods for producing or enhancing the production of group II intron RNP particles in eukaryotic cells. The present methods comprise introducing at least one nucleic acid construct comprising a nucleic acid encoding a modified or wild type group II intron RNA and a wild-type or modified group II intron-encoded protein into the eukaryotic cell, and maintaining the cell under conditions that allow for expression of the group II intron RNA and the group II intron-encoded protein in the cell. The nucleic acid encoding the group II intron RNA is operably linked to an RNA polymerase I, an RNA polymerase II, or an RNA polymerase III promoter, and the nucleic acid encoding the group II intron-encoded protein is operably linked to an RNA polymerase II promoter. In certain embodiments, a subcellular localization signal is attached to the group II intron-encoded protein.
摘要翻译：本文提供了核酸构建体和用于在真核细胞中产生或增强II组内含子RNP颗粒的生产的方法。本发明的方法包括将包含编码经修饰或野生型II组内含子RNA的核酸和野生型或修饰II型内含子编码蛋白质的至少一种核酸构建体引入真核细胞中，并将细胞维持在允许在细胞中表达II组内含子RNA和II组内含子编码的蛋白质。编码II组内含子RNA的核酸可操作地连接到RNA聚合酶I，RNA聚合酶II或RNA聚合酶III启动子，编码II组内含子编码蛋白的核酸可操作地连接到RNA聚合酶II 子。在某些实施方案中，将亚细胞定位信号连接到II组内含子编码的蛋白质。

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