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1. 发明授权

US06953692B2 Device and method for seed-train expansion of mammalian cells 有权
标题翻译：哺乳动物细胞种子培养扩增的装置和方法
公开(公告)号：US06953692B2
公开(公告)日：2005-10-11
申请号：US10029779
申请日：2001-12-18
申请人： Rudiger Heidemann , Mokhtar Mered , James D. Michaels , Konstantin Konstantinov
发明人： Rudiger Heidemann , Mokhtar Mered , James D. Michaels , Konstantin Konstantinov
IPC分类号： C12M1/36 , C12M3/00 , C12N5/00 , C12N5/02
CPC分类号： C12M41/26 , C12M33/00 , C12M41/12 , C12M41/34 , C12M41/46
摘要： A device and method for seed-train expansion of a source of mammalian cells and their use in production of proteins expressed by the cells comprising the use of a dedicated inoculation bioreactor for expanding the cells prior to the transfer to a production bioreactor is disclosed.The novel inoculation bioreactor is designed to facilitate an improved method of mammalian cell seed-train expansion, and is distinguished by the presence of an “inoculation well” which communicates with the interior of the bioreactor and which facilitates the growth of mammalian cells for commercial seed-train expansion.The method comprises adding the cryopreserved cells to media within the inoculation well of the inoculation bioreactor, enabling the cells to grow to a predetermined concentration within the inoculation well by monitoring and adjusting the condition of the media and environment, and thereafter incrementally increasing the volume of the media within the reactor so that optimal cell growth is maintained.
摘要翻译：公开了用于种子培养哺乳动物细胞源的装置和方法及其在生产由细胞表达的蛋白质中的用途，所述细胞包括在转移至生产生物反应器之前使用专用接种生物反应器来扩增细胞。新颖的接种生物反应器设计用于促进哺乳动物细胞种子培养扩展的改进方法，并且通过存在与生物反应器内部连通的“接种孔”的区别，并且促进用于商业种子的哺乳动物细胞的生长火车扩张。该方法包括将冷冻保存的细胞加入到接种生物反应器的接种孔内的培养基中，使细胞通过监测和调节培养基和环境的状况使细胞在接种孔内生长至预定浓度，然后逐渐增加反应器内的培养基，使得保持最佳的细胞生长。

2. 发明授权

US06670175B2 Cryopreservation bag assembly for mammalian cell lines 有权
公开(公告)号：US06670175B2
公开(公告)日：2003-12-30
申请号：US10143194
申请日：2002-05-09
申请人： De Qian Wang , Bruce Gardner , Rudiger Heidemann , Mokhtar Mered , William H. Kelsey
发明人： De Qian Wang , Bruce Gardner , Rudiger Heidemann , Mokhtar Mered , William H. Kelsey
IPC分类号： C12M100
CPC分类号： A61J1/10 , C12M45/22 , C12M47/20 , Y10S128/24
摘要： The invention is a novel cell freezing and storage bag assembly and method for using the assembly in the seed train expansion of mammalian cells. The bag is constructed principally of polytetrafluoroethylene fabric. The bag is designed to hold enough cells that a bioreactor can be inoculated directly. The bag is designed to be filled to a fraction of its maximum capacity so that the cell suspension has a very thin cross-section (less than about 10 millimeters). The bag design includes a transfer set that can be sterilely welded to the source of the mammalian cells. This sterile-weldable transfer set allows the bags to be filled quickly with minimal risk of contamination. In the method, once each bag is filled, it is sealed below the connection with the transfer set and the bag is cut “above” the new seal (on the same side of the seal as the transfer set). When a bioreactor is to be inoculated, the contents of the bag are drained via a sterile-weldable inoculation line. During freezing and storage, the inoculation line is protected from mechanical damage.

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