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1. 发明授权

US06905850B2 Process for the production of glycerides with lipases 有权
标题翻译：用脂肪酶生产甘油酯的方法
公开(公告)号：US06905850B2
公开(公告)日：2005-06-14
申请号：US10311350
申请日：2001-07-02
申请人： Roxana Irimescu , Kiyomi Furihata , Kazuhiko Hata , Tsuneo Yamane
发明人： Roxana Irimescu , Kiyomi Furihata , Kazuhiko Hata , Tsuneo Yamane
IPC分类号： C11C3/08 , C11C3/10 , C12P7/64
CPC分类号： C12P7/6445 , C12P7/6472
摘要： The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency.The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The “first lipase” refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the “second lipase” refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.
摘要翻译：本发明提供了以非常高的纯度和高效率生产2-单甘油酯（2-MG）或甘油三酸酯（TG），特别是在sn-2位具有多不饱和脂肪酸（PUFA）残基的甘油三酯）的方法。通过用第一种1,3-脂肪酶进行起始TG的醇解来制备2-MG。然后，通过使用第二种1,3-脂肪酶将所产生的2-MG的1-和3-位引入脂肪酸残基来产生TG。 2-位的脂肪酸残基优选为DHA，EPA或ARA的残基。 “第一脂肪酶”是指对于1-和3-位选择性的那些，并且甚至可以在醇解中的长链脂肪酸上起作用，而“第二脂肪酶”是指对于1-和 3-位，并且可以通过2-MG与脂肪酸酯或游离脂肪酸的反应产生TG。

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