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1. 发明授权

US5260441A Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies 失效
公开(公告)号：US5260441A
公开(公告)日：1993-11-09
申请号：US316846
申请日：1989-02-28
申请人： Daniel F. Heiman , Daniel S. Raden , Robert E. Dubler
发明人： Daniel F. Heiman , Daniel S. Raden , Robert E. Dubler
IPC分类号： G01N33/53 , C07D489/00 , C07D489/02 , C07D489/08 , C07D493/10 , C07D519/00 , G01N33/94
CPC分类号： G01N33/9486 , C07D489/00 , C07D489/02 , C07D489/08 , Y10S436/80 , Y10S436/805 , Y10S436/825 , Y10S436/826 , Y10S530/807
摘要： The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

2. 发明授权

US5239086A Haptens and tracers for immunoassays for propoxyphene 失效
标题翻译：用于自发免疫的用于丙氧基的用户和追踪者
公开(公告)号：US5239086A
公开(公告)日：1993-08-24
申请号：US736890
申请日：1991-07-29
申请人： Robert E. Dubler , Jonathan Grote , Donna R. Kuhn
发明人： Robert E. Dubler , Jonathan Grote , Donna R. Kuhn
IPC分类号： G01N33/53 , C07K16/00 , C07K16/44 , G01N33/531 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： G01N33/533 , C07K16/44 , G01N33/9486 , Y10S436/815
摘要： Disclosed is a substantially optically pure hapten, useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene. The hapten corresponds to a specified structural formula (IX).Also disclosed is an immunogen derived from the hapten as well as an antibody raised in response to an immunogen derived from the hapten.Also disclosed is a fluorescent tracer derived from a substantially optically pure compound corresponding to the hapten, the tracer being useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene.Also disclosed is an improved immunoassay for determining dextropropoxyphene and/or nordextropropoxyphene in a biological sample involving a step of contacting the sample with antibodies raised in response to the immunogen. Also disclosed is a fluorescence polarization immunoassay (FPIA) for determining dextropropoxyphene and/or nordextropropoxyphene involving a step of contacting the sample with antibodies raised in response to the immunogen, and/or involving a step of contacting the sample with a fluorescent tracer.
摘要翻译：公开了一种基本上光学纯的半抗原，其可用于右旋丙氧芬和/或右丙氧吩芬的免疫测定。半抗原对应于指定的结构式（IX）。还公开了衍生自半抗原的免疫原以及响应于来自半抗原的免疫原产生的抗体。还公开了衍生自对应于半抗原的基本上光学纯的化合物的荧光示踪剂，该示踪剂可用于对右丙氧芬和/或右丙氧吩芬的免疫测定中。还公开了用于测定生物样品中的右旋丙氧芬和/或非诺酮丙氧芬的改进的免疫测定，其涉及将样品与响应于免疫原产生的抗体接触的步骤。还公开了用于测定右旋丙氧芬和/或右丙氧吩的荧光偏振免疫测定（FPIA），其涉及使样品与响应于免疫原而产生的抗体接触的步骤和/或涉及使样品与荧光示踪剂接触的步骤。

3. 发明授权

US5155212A Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit 失效
标题翻译：苯环利定和苯环利定代谢物测定，示踪剂，免疫原，抗体和试剂盒
公开(公告)号：US5155212A
公开(公告)日：1992-10-13
申请号：US529988
申请日：1990-05-29
申请人： Robert E. Dubler , Mary P. Frintner , Jonathan Grote , Gregg A. Hadley , David J. Hawksworth , Hal D. Hopkins , Daniel S. Nam , Frank S. Ungemach , Larry K. Wray
发明人： Robert E. Dubler , Mary P. Frintner , Jonathan Grote , Gregg A. Hadley , David J. Hawksworth , Hal D. Hopkins , Daniel S. Nam , Frank S. Ungemach , Larry K. Wray
IPC分类号： A61K39/395 , A61K49/00 , C07D211/06 , C07D211/46 , C07D211/58 , C07D211/74 , C07D295/02 , C07D295/033 , C07D295/14 , C07D295/155 , C07D401/12 , C07D493/10 , C07K14/00 , C07K16/00 , C07K16/44 , C09B11/28 , C09K11/06 , C12N5/10 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/53 , G01N33/542 , G01N33/58 , G01N33/94
CPC分类号： G01N33/9486 , C07D211/46 , C07D211/58 , C07D211/74 , C07D295/033 , C07D295/155 , C07D401/12 , C07D493/10 , C07K16/44 , G01N33/582 , C07C2101/14 , Y10S436/816 , Y10S530/807
摘要： The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparting and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.
摘要翻译：本发明涉及苯环利定和苯环利定衍生物对于制备和进行这种测定所需的各种成分的荧光偏振测定，以及制备这些组分的方法。具体地，公开了示踪剂，免疫原和抗体，以及制备它们的方法和含有它们的试剂盒。示踪剂和免疫原由取代的苯环利定化合物制成。荧光素部分包含在示踪剂中，而聚（氨基酸）形成免疫原的一部分。该测定通过测量已经通过含有抗血清和示踪剂的样品的平面偏振光的偏振保持程度来进行。

4. 发明授权

US5463027A Fluorescence polarazation immunoassay for tetrahydrocannabinoids 失效
标题翻译：四氢大麻素荧光偏振免疫测定
公开(公告)号：US5463027A
公开(公告)日：1995-10-31
申请号：US288175
申请日：1994-08-10
申请人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
发明人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
IPC分类号： C07D311/80 , C07D491/04 , C07D493/10 , C07F7/08 , G01N33/533 , G01N33/542 , G01N33/94 , C07D441/16 , C09B29/033 , C01K3/00
CPC分类号： C07D491/04 , C07D311/80 , C07D493/10 , C07F7/0812 , G01N33/533 , G01N33/542 , G01N33/948 , Y10S436/80 , Y10S436/805 , Y10S436/815 , Y10S530/802
摘要： This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.
摘要翻译：本公开涉及生物流体如尿中用于测定四氢大麻素（THC）和THC代谢物的方法和试剂。特别地，本公开内容涉及用于确定THC的存在的荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所述方法还提供了用于THC荧光偏振测定的新型洗涤剂。

5. 发明授权

US5258512A Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies 失效
标题翻译：鸦片生物碱及其代谢物的免疫测定; 示踪剂，免疫原和抗体
公开(公告)号：US5258512A
公开(公告)日：1993-11-02
申请号：US940589
申请日：1992-09-04
申请人： Daniel F. Heiman , Daniel S. Raden , Robert E. Dubler
发明人： Daniel F. Heiman , Daniel S. Raden , Robert E. Dubler
IPC分类号： C07D489/00 , C07D489/02 , C07D489/08 , C07D519/00 , G01N33/94
CPC分类号： G01N33/9486 , C07D489/00 , C07D489/02 , C07D489/08
摘要： The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.
摘要翻译：本发明涉及鸦片类生物碱及其代谢产物的荧光偏振测定，制备和进行这种测定所需的各种组分以及制备这些组分的方法。具体地，公开了示踪剂，免疫原和抗体，以及制备它们的方法。示踪剂和免疫原由取代的阿片碱生物碱制成。示踪剂中包含荧光素部分，而聚（氨基酸）形成免疫原的一部分。通过测量当用平面偏振光照射与抗血清和示踪剂混合的样品时产生的荧光的偏振保持程度来进行测定。

6. 发明授权

US5407834A Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit 失效
标题翻译：苯环利定和苯环利定代谢物测定，示踪剂，免疫原，抗体和试剂盒
公开(公告)号：US5407834A
公开(公告)日：1995-04-18
申请号：US831762
申请日：1992-04-27
申请人： Robert E. Dubler , Mary P. Frintner , Jonathan Grote , Gregg A. Hadley , David J. Hawksworth , Hal D. Hopkins , Daniel S. Nam , Frank S. Ungemach , Larry K. Wray
发明人： Robert E. Dubler , Mary P. Frintner , Jonathan Grote , Gregg A. Hadley , David J. Hawksworth , Hal D. Hopkins , Daniel S. Nam , Frank S. Ungemach , Larry K. Wray
IPC分类号： A61K39/395 , A61K49/00 , C07D211/06 , C07D211/46 , C07D211/58 , C07D211/74 , C07D295/02 , C07D295/033 , C07D295/14 , C07D295/155 , C07D401/12 , C07D493/10 , C07K14/00 , C07K16/00 , C07K16/44 , C09B11/28 , C09K11/06 , C12N5/10 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/53 , G01N33/542 , G01N33/58 , G01N33/94 , C12N5/20 , G01N33/577 , C07K15/28
CPC分类号： G01N33/9486 , C07D211/46 , C07D211/58 , C07D211/74 , C07D295/033 , C07D295/155 , C07D401/12 , C07D493/10 , C07K16/44 , G01N33/582 , C07C2101/14 , Y10S436/816 , Y10S530/807
摘要： The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.
摘要翻译：本发明涉及苯环利定和苯环利定衍生物的荧光偏振测定，制备和进行这种测定所需的各种组分，以及制备这些组分的方法。具体地，公开了示踪剂，免疫原和抗体，以及制备它们的方法和含有它们的试剂盒。示踪剂和免疫原由取代的苯环利定化合物制成。荧光素部分包含在示踪剂中，而聚（氨基酸）形成免疫原的一部分。该测定通过测量已经通过含有抗血清和示踪剂的样品的平面偏振光的偏振保持程度来进行。

7. 发明授权

US5073629A Methadone fluorescence polarization immunoassay 失效
标题翻译：甲基荧光偏振免疫
公开(公告)号：US5073629A
公开(公告)日：1991-12-17
申请号：US300670
申请日：1989-01-23
申请人： Robert E. Dubler , Susan A. Thacker , John A. Walling , Nai-Yi Wang
发明人： Robert E. Dubler , Susan A. Thacker , John A. Walling , Nai-Yi Wang
IPC分类号： G01N33/53 , C07C49/215 , C07C59/84 , C07C225/16 , C07C235/70 , C07C237/04 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： G01N33/542 , G01N33/533 , G01N33/9486
摘要： A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.

8. 发明授权

US5356820A Haptens, tracers, immunogens and antibodies for immunoassays for propoxyphene 失效
标题翻译：用于丙氧芬的免疫测定的半抗原，示踪剂，免疫原和抗体
公开(公告)号：US5356820A
公开(公告)日：1994-10-18
申请号：US60937
申请日：1993-05-12
申请人： Robert E. Dubler , Jonathan Grote , Donna R. Kuhn
发明人： Robert E. Dubler , Jonathan Grote , Donna R. Kuhn
IPC分类号： G01N33/53 , C07K16/00 , C07K16/44 , G01N33/531 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： G01N33/533 , C07K16/44 , G01N33/9486 , Y10S436/815
摘要： Disclosed is a substantially optically pure hapten, useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene. The hapten corresponds to a specified structural formula (IX).Also disclosed is an immunogen derived from the hapten as well as an antibody raised in response to an immunogen derived from the hapten.Also disclosed is a fluorescent tracer derived from a substantially optically pure compound corresponding to the hapten, the tracer being useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene.Also disclosed is an improved immunoassay for determining dextropropoxyphene and/or nordextropropoxyphene in a biological sample involving a step of contacting the sample with antibodies raised in response to the immunogen. Also disclosed is a fluorescence polarization immunoassay (FPIA) for determining dextropropoxyphene and/or nordextropropoxyphene involving a step of contacting the sample with antibodies raised in response to the immunogen, and/or involving a step of contacting the sample with a fluorescent tracer.
摘要翻译：公开了一种基本上光学纯的半抗原，其可用于右旋丙氧芬和/或右丙氧吩芬的免疫测定。半抗原对应于指定的结构式（IX）。还公开了衍生自半抗原的免疫原以及响应于来自半抗原的免疫原产生的抗体。还公开了衍生自对应于半抗原的基本上光学纯的化合物的荧光示踪剂，该示踪剂可用于对右丙氧芬和/或右丙氧吩芬的免疫测定中。还公开了用于测定生物样品中的右旋丙氧芬和/或非诺酮丙氧芬的改进的免疫测定，其涉及将样品与响应于免疫原产生的抗体接触的步骤。还公开了用于测定右旋丙氧芬和/或右丙氧吩的荧光偏振免疫测定（FPIA），其涉及使样品与响应于免疫原而产生的抗体接触的步骤和/或涉及使样品与荧光示踪剂接触的步骤。

9. 发明授权

US5264373A Fluorescence polarization immunoassay for tetrahydrocannabinoids 失效
标题翻译：四氢大麻素的荧光偏振免疫测定
公开(公告)号：US5264373A
公开(公告)日：1993-11-23
申请号：US938980
申请日：1992-09-01
申请人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
发明人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
IPC分类号： C07D311/80 , C07D491/04 , C07D493/10 , C07F7/08 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： C07D491/04 , C07D311/80 , C07D493/10 , C07F7/0812 , G01N33/533 , G01N33/542 , G01N33/948 , Y10S436/80 , Y10S436/805 , Y10S436/815 , Y10S530/802
摘要： This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.
摘要翻译：本公开涉及生物流体如尿中用于测定四氢大麻素（THC）和THC代谢物的方法和试剂。特别地，本公开内容涉及用于确定THC的存在的荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所述方法还提供了用于THC荧光偏振测定的新型洗涤剂。

10. 发明授权

US5144030A Fluorescene polarization immunoassay for tetrahydrocannabinoids 失效
标题翻译：四氢大麻素的荧光偏振免疫测定
公开(公告)号：US5144030A
公开(公告)日：1992-09-01
申请号：US366266
申请日：1989-06-12
申请人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
发明人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
IPC分类号： G01N33/53 , C07D311/58 , C07D311/80 , C07D491/04 , C07D491/052 , C07D493/10 , C07D519/00 , C07F7/08 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： C07D491/04 , C07D311/80 , C07D493/10 , C07F7/0812 , G01N33/533 , G01N33/542 , G01N33/948
摘要： This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.
摘要翻译：本公开涉及生物流体如尿中用于测定四氢大麻素（THC）和THC代谢物的方法和试剂。特别地，本公开内容涉及用于确定THC的存在的荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所述方法还提供了用于THC荧光偏振测定的新型洗涤剂。

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