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1. 发明授权

US4207394A Process and apparatus for analyzing specimens for the presence of microorganisms therein 失效
标题翻译：用于分析样品中微生物存在的方法和设备
公开(公告)号：US4207394A
公开(公告)日：1980-06-10
申请号：US854592
申请日：1977-11-25
申请人： Clifton Aldridge, Jr. , Paul W. Jones , Sandra F. Gibson , Richard D. Vannest , James T. Holen , George F. Keyser , Michael C. Meyer
发明人： Clifton Aldridge, Jr. , Paul W. Jones , Sandra F. Gibson , Richard D. Vannest , James T. Holen , George F. Keyser , Michael C. Meyer
IPC分类号： B01L3/00 , C12M1/34 , C12Q1/04 , G01N1/38 , G01N35/00 , C12M1/18
CPC分类号： B01L3/502 , C12M41/36 , C12Q1/04 , G01N2001/385 , G01N2035/00148 , G01N2035/00326
摘要： Microorganisms in a specimen are detected, identified, and enumerated by introducing the specimen into a sampling cartridge and diluting the specimen with a known volume of water within the cartridge. The cartridge has a manifold and several cassettes attached to the manifold. Each cassette contains a serpentine flow channel having a series of filters therein and a detection cell located downstream from each filter. The flow channel in each cassette also contains a culture medium which is freeze dried and is highly selective in the sense that it promotes the growth of one type of microorganism, but not others. The mixture of the specimen and water flows from the manifold into the flow channel of each cassette where it rehydrates the culture medium therein and further flows through the filters. Each filter removes a known proportion of the microorganisms from the mixture of specimen, water and medium, thereby effecting a serial dilution. After the cassettes are heated to incubate the microoganisms, the detection cells are observed for growth of the microorganisms therein which is manifested in a change in the light transmitting characteristics of the mixtures within the cells.
摘要翻译：样本中的微生物通过将样品引入取样筒中并用筒内已知体积的水稀释样品来检测，鉴定和列举。墨盒具有歧管和附接到歧管的多个盒。每个盒包含蛇形流动通道，其中具有一系列过滤器，并且位于每个过滤器下游的检测单元。每个盒中的流动通道还含有冷冻干燥并且在促进一种类型的微生物生长而不是其他微生物的意义上是高选择性的培养基。样品和水的混合物从歧管流入每个盒的流动通道，其中其中的培养基再水合并进一步流过过滤器。每个过滤器从样品，水和介质的混合物中除去已知比例的微生物，从而进行连续稀释。在将盒加热以孵育微生物之后，观察检测细胞的微生物生长，其表现为细胞内混合物的透光特性的变化。

2. 发明授权

US4173515A Group D enterococci broth 失效
标题翻译： D组肠球蛋白
公开(公告)号：US4173515A
公开(公告)日：1979-11-06
申请号：US792715
申请日：1977-05-02
申请人： Clifton Aldridge , Sandra F. Gibson , Richard D. Vannest , Gregory D. Rodgers
发明人： Clifton Aldridge , Sandra F. Gibson , Richard D. Vannest , Gregory D. Rodgers
IPC分类号： C12Q1/10 , C12K1/10
CPC分类号： C12Q1/10 , G01N2333/31
摘要： A broth medium for the detection of Group D enterococci in urine. The medium employs neomycin sulfate to inhibit growth of Staphylococcus aureus, potassium tellurite to inhibit growth of gram-negative organisms, and ferric ammonium citrate and esculin hydrolysate indicators.
摘要翻译：用于检测尿中D组肠球菌的肉汤培养基。该培养基采用硫酸新霉素抑制金黄色葡萄球菌的生长，钾碲酸盐抑制革兰氏阴性生物的生长，以及柠檬酸铁铵和七叶树素水解物指示剂。

3. 发明授权

US3963355A Process and apparatus for analyzing specimens for the presence of microorganisms therein 失效
标题翻译：用于分析样品中微生物存在的方法和设备
公开(公告)号：US3963355A
公开(公告)日：1976-06-15
申请号：US461249
申请日：1974-04-16
申请人： Clifton Aldridge, Jr. , Paul W. Jones , Sandra F. Gibson , Richard D. Vannest , James T. Holen , George F. Keyser , Michael C. Meyer
发明人： Clifton Aldridge, Jr. , Paul W. Jones , Sandra F. Gibson , Richard D. Vannest , James T. Holen , George F. Keyser , Michael C. Meyer
IPC分类号： C12M1/34 , G01N35/00 , G01N21/06 , G01N1/00
CPC分类号： C12Q1/04 , B01L3/502 , C12M41/36 , B01L2200/028 , B01L2300/0681 , B01L2300/0864 , B01L2400/049 , G01N2035/00148 , Y10S435/808 , Y10S435/875 , Y10S435/879 , Y10S435/883 , Y10S435/885 , Y10S435/922
摘要： Microorganisms in a specimen are detected, identified, and enumerated by introducing the specimen into a sampling cartridge and diluting the specimen with a known volume of water within the cartridge. The cartridge has a manifold and several cassettes attached to the manifold. Each cassette contains a serpentine flow channel having a series of filters therein and a detection cell located downstream from each filter. The flow channel in each cassette also contains a culture medium which is freeze dried and is highly selective in the sense that it promotes the growth of one type of microorganism, but not others. The mixture of the specimen and water flows from the manifold into the flow channel of each cassette where it rehydrates the culture medium therein and further flows through the filters. Each filter removes a known proportion of the microorganisms from the mixture of specimen, water and medium, thereby effecting a serial dilution. After the cassettes are heated to incubate the microorganisms, the detection cells are observed for growth of the microorganisms therein which is manifested in a change in the light transmitting characteristics of the mixtures within the cells. This change is detected with great precision by projecting beams of light through the detection cells and measuring the current flowing through dedicated photocells located beyond the detection cells. The amount of current flowing through each photocell is proportional to the relative optical density of the mixture within the corresponding detection cell, and this density is plotted against time to give a unique time-related signature for each microorganism. Thus, the presence and identity of microorganisms in the detection cells is determined by mere changes in the optical density of the cells, whereas the number of microorganisms may be determined by considering the number of detection cells which change.
摘要翻译：样本中的微生物通过将样品引入取样筒中并用筒内已知体积的水稀释样品来检测，鉴定和列举。墨盒具有歧管和附接到歧管的多个盒。每个盒包含蛇形流动通道，其中具有一系列过滤器，并且位于每个过滤器下游的检测单元。每个盒中的流动通道还含有冷冻干燥并且在促进一种类型的微生物生长而不是其他微生物的意义上是高选择性的培养基。样品和水的混合物从歧管流入每个盒的流动通道，其中其中的培养基再水合并进一步流过过滤器。每个过滤器从样品，水和介质的混合物中除去已知比例的微生物，从而进行连续稀释。在盒被加热以孵育微生物之后，观察检测细胞以生长其中的微生物，其表现为细胞内混合物的透光特性的变化。通过将光束投影通过检测单元并测量流过位于检测单元之外的专用光电池的电流，可以很好地检测该变化。流过每个光电池的电流量与相应检测单元内的混合物的相对光密度成比例，并且将该密度与时间作图，以给出每个微生物独特的时间相关特征。因此，检测细胞中的微生物的存在和身份通过细胞的光密度的改变来确定，而可以通过考虑改变的检测细胞的数量来确定微生物的数量。

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