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1. 发明授权

US09295237B2 Recombinant constructs and transgenic fluorescent ornamental fish therefrom 有权
公开(公告)号：US09295237B2
公开(公告)日：2016-03-29
申请号：US12577467
申请日：2009-10-12
申请人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
IPC分类号： A01K67/027 , A01K67/00 , C07K14/435 , C12N15/85 , G06Q99/00
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2217/052 , A01K2217/206 , A01K2227/40 , A01K2267/0393 , C07K14/43595 , C12N15/8509 , C12N2830/008 , G06Q99/00
摘要： The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

2. 发明申请

US20120317665A1 Recombinant Constructs And Purple Transgenic Fluorescent Ornamental Fish Therefrom 有权
标题翻译：重组构建体和紫色转基因荧光观赏鱼
公开(公告)号：US20120317665A1
公开(公告)日：2012-12-13
申请号：US13561615
申请日：2012-07-30
申请人： Alan Blake , Richard Crockett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Aidas Nasevicius
IPC分类号： A01K67/027 , A01K61/00 , A01K67/02
CPC分类号： C07K14/43504 , A01K67/0275 , A01K2217/052 , A01K2227/40 , A01K2267/02
摘要： The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
摘要翻译：本发明涉及荧光蛋白在制备紫色转基因荧光鱼中的应用和方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。因此，开发出来自新颖来源的不同荧光颜色的观赏鱼的新品种。

3. 发明授权

US08232450B1 Purple transgenic fluorescent ornamental fish 有权
标题翻译：紫色转基因荧光观赏鱼
公开(公告)号：US08232450B1
公开(公告)日：2012-07-31
申请号：US13396382
申请日：2012-02-14
申请人： Alan Blake , Richard Crockett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Aidas Nasevicius
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： C07K14/43504 , A01K67/0275 , A01K2217/052 , A01K2227/40 , A01K2267/02
摘要： The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
摘要翻译：本发明涉及荧光蛋白在制备紫色转基因荧光鱼中的应用和方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。因此，开发出来自新颖来源的不同荧光颜色的观赏鱼的新品种。

4. 发明授权

US5462080A Heated removable throttle valve 失效
标题翻译：加热可拆卸节流阀
公开(公告)号：US5462080A
公开(公告)日：1995-10-31
申请号：US114893
申请日：1993-08-31
申请人： Richard W. Plavidal , Petru N. Nitescu , Greg Mudwilder , Richard Crockett
发明人： Richard W. Plavidal , Petru N. Nitescu , Greg Mudwilder , Richard Crockett
IPC分类号： F16K1/22 , F16K27/02 , F16K47/08 , F16K49/00 , F16K51/02
CPC分类号： F16K27/0218 , F16K47/08 , F16K49/002 , Y10T137/6048 , Y10T137/6497 , Y10T137/6606 , Y10T137/8122
摘要： A two piece throttling valve is disclosed. The valve assembly includes a stationary outer housing with an inner removable operable assembly. In this configuration the piping (pump stack) does not have to disassembled to remove, clean or replace the internal valve pieces or the in-line screen. The inner assembly can be quickly removed for cleaning. The entire valve assembly is heated to reduce deposition of volatile process byproducts on the internal valve surfaces and reduce the cleaning frequency. A wedge plate is provided in the outer stationary valve housing to mate with a sloped bottom surface of the inner valve housing such that when the inner assembly is in position in the outer valve housing there is a tight fit between adjacent sealing surfaces around the valve bore. A single flat surface seal provides vacuum sealing between the inner assembly and outer housing. A dual valve stem (shaft) seal seals the valve shaft. The seal includes an inner O-ring made of a chemically resistant material and an outer O-ring that is less chemically resistant, but has a dual-quad profile to prevent vacuum leakage. A screen in the fluid passage adjacent to and supported by the outer housing can easily be accessed from the inside of the outer housing.
摘要翻译：公开了一种两件式节流阀。阀组件包括具有内部可拆卸可操作组件的固定外壳。在这种配置中，管道（泵组）不需要拆卸，以拆卸，清洁或更换内部阀片或直列式屏幕。内部组件可以快速拆卸清洁。加热整个阀门组件以减少内部阀门表面上挥发性过程副产物的沉积并降低清洗频率。在外部固定阀壳体中设置楔形板，以与内部阀壳体的倾斜的底部表面配合，使得当内部组件在外部阀壳体中的适当位置时，在阀孔周围的相邻密封表面之间紧密配合。单个平面密封件在内部组件和外部壳体之间提供真空密封。双阀杆（轴）密封件密封阀轴。密封件包括由耐化学腐蚀材料制成的内O形环和外部O形环，其耐化学性较差，但具有双重四边形以防止真空泄漏。靠近外壳的流体通道中的屏幕可以容易地从外壳的内部进入。

5. 发明申请

US20120210454A1 BLUE TRANSGENIC FLUORESCENT ORNAMENTAL FISH 有权
标题翻译：蓝色转基因荧光鱼
公开(公告)号：US20120210454A1
公开(公告)日：2012-08-16
申请号：US13396471
申请日：2012-02-14
申请人： Alan BLAKE , Richard CROCKETT , Aidas NASEVICIUS
发明人： Alan BLAKE , Richard CROCKETT , Aidas NASEVICIUS
IPC分类号： A01K67/027 , A01K61/00 , A01K67/02
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2267/02
摘要： The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
摘要翻译：本发明涉及转基因蓝色观赏鱼，以及通过体外受精技术制备这种鱼的方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。

6. 发明授权

US08232451B1 Blue transgenic fluorescent ornamental fish 有权
标题翻译：蓝色转基因荧光观赏鱼
公开(公告)号：US08232451B1
公开(公告)日：2012-07-31
申请号：US13396471
申请日：2012-02-14
申请人： Alan Blake , Richard Crockett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Aidas Nasevicius
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2267/02
摘要： The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
摘要翻译：本发明涉及转基因蓝色观赏鱼，以及通过体外受精技术制造这种鱼的方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。

7. 发明申请

US20100145889A1 RECOMBINANT CONSTRUCTS AND TRANSGENIC FLUORESCENT ORNAMENTAL FISH THEREFROM 审中-公开
公开(公告)号：US20100145889A1
公开(公告)日：2010-06-10
申请号：US12705852
申请日：2010-02-15
申请人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
IPC分类号： G06Q90/00 , C12N15/85
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2217/052 , A01K2217/206 , A01K2227/40 , A01K2267/0393 , C07K14/43595 , C12N15/8509 , C12N2830/008 , G06Q99/00
摘要： The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

8. 发明授权

US08581024B2 Blue transgenic fluorescent ornamental fish 有权
标题翻译：蓝色转基因荧光观赏鱼
公开(公告)号：US08581024B2
公开(公告)日：2013-11-12
申请号：US13561679
申请日：2012-07-30
申请人： Alan Blake , Richard Crockett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Aidas Nasevicius
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2267/02
摘要： The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
摘要翻译：本发明涉及转基因蓝色观赏鱼，以及通过体外受精技术制备这种鱼的方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。

9. 发明申请

US20120210453A1 PURPLE TRANSGENIC FLUORESCENT ORNAMENTAL FISH 有权
标题翻译：紫外线荧光免疫荧光鱼
公开(公告)号：US20120210453A1
公开(公告)日：2012-08-16
申请号：US13396382
申请日：2012-02-14
申请人： Alan BLAKE , Richard CROCKETT , Aidas NASEVICIUS
发明人： Alan BLAKE , Richard CROCKETT , Aidas NASEVICIUS
IPC分类号： A01K67/027 , A01K61/00 , A01K67/02
CPC分类号： C07K14/43504 , A01K67/0275 , A01K2217/052 , A01K2227/40 , A01K2267/02
摘要： The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
摘要翻译：本发明涉及荧光蛋白在制备紫色转基因荧光鱼中的应用和方法。还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。因此，开发出来自新颖来源的不同荧光颜色的观赏鱼的新品种。

10. 发明授权

US07700825B2 Recombinant constructs and transgenic fluorescent ornamental fish therefrom 有权
标题翻译：重组构建体和转基因荧光观赏鱼
公开(公告)号：US07700825B2
公开(公告)日：2010-04-20
申请号：US11839364
申请日：2007-08-15
申请人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
发明人： Alan Blake , Richard Crockett , Jeffrey Essner , Perry Hackett , Aidas Nasevicius
IPC分类号： A01K67/027 , A01K67/00 , A01K67/033 , C12N15/00
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2217/052 , A01K2217/206 , A01K2227/40 , A01K2267/0393 , C07K14/43595 , C12N15/8509 , C12N2830/008 , G06Q99/00
摘要： The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
摘要翻译：本发明涉及珊瑚珊瑚荧光蛋白在制备转基因红，绿和黄荧光斑马鱼中的方法和应用。优选地，这种荧光斑马鱼是可育的并且用于建立转基因斑马鱼群体，并为了营销目的提供给观赏鱼业。因此，开发出来自新颖来源的不同荧光颜色的观赏鱼的新品种。

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