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    • 4. 发明申请
    • Method for assessment of particles
    • 粒子评估方法
    • US20060063146A1
    • 2006-03-23
    • US10533324
    • 2003-10-31
    • Rasmus LarsenFrans Ejner Hansen
    • Rasmus LarsenFrans Ejner Hansen
    • C12Q1/70C12Q1/68G01N33/53G01N33/567
    • G01N33/54313
    • The invention relates to imaging methods for assessing quality or quantity parameters of particles in a sample, wherein the particles contain less than 106 analyte detectable positions. The method comprises 1) mixing the sample with a targeting species capable of binding an analyte position and a labelling agent, 2) arranging the sample in an exposing domain, allowing electromagnetic signals from the sample to pass to the exterior, 3) exposing a representation of said signals onto an array of detection elements, wherein the representation is subject to a linear enlargement, so that the ratio of the image of a linear dimension on the array of detection elements to the original linear dimension in the exposing domain is smaller than 20:1, 4) detecting the representation as intensities by said detection elements, 5) processing the intensities to identify the particles, and 6) obtaining the quality or quantity parameter.
    • 本发明涉及用于评估样品中颗粒的质量或数量参数的成像方法,其中所述颗粒含有小于10 6个分析物的可检测位置。 该方法包括:1)将样品与能够结合分析物位置的靶向物质和标记试剂混合,2)将样品置于曝光区域,允许来自样品的电磁信号传递到外部,3) 的所述信号传送到检测元件的阵列上,其中所述表示经历线性放大,使得检测元件阵列上的线性尺寸的图像与曝光区域中的原始线性尺寸的比率小于20 :1,4)通过所述检测元件检测所述表示为强度,5)处理所述强度以识别所述粒子,以及6)获得所述质量或数量参数。