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1. 发明授权

US08148602B2 Diacylglycerol acyltransferases from flax 有权
标题翻译：来自亚麻的二酰基甘油酰基转移酶
公开(公告)号：US08148602B2
公开(公告)日：2012-04-03
申请号：US12381183
申请日：2009-03-09
申请人： Randall Weselake , Rodrigo Siloto , Qin Liu , André Laroche , Eric Murphy , Kimmo Koivu
发明人： Randall Weselake , Rodrigo Siloto , Qin Liu , André Laroche , Eric Murphy , Kimmo Koivu
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , A01H15/00 , C12N5/14 , C12N15/82 , C07H21/04
CPC分类号： C12P7/6463 , C12N9/1029 , C12N15/8247
摘要： The invention relates to isolated diacylglycerol acyltransferases and polynucleotide sequences encoding the DGAT enzymes; polynucleotide constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same. Also provided are transformed cells and transgenic plants, especially Camelina sativa plant, with enhanced oil accumulation and quality.
摘要翻译：本发明涉及分离的二酰基甘油酰基转移酶和编码DGAT酶的多核苷酸序列; 多核苷酸构建体，载体和掺入多核苷酸序列的宿主细胞; 以及其制造和使用方法。还提供转化细胞和转基因植物，特别是Camelina sativa植物，具有增强的油积聚和质量。

2. 发明授权

US08063269B2 Transgenic plants with reduced level of saturated fatty acid and methods for making them 失效
标题翻译：具有降低饱和脂肪酸水平的转基因植物及其制备方法
公开(公告)号：US08063269B2
公开(公告)日：2011-11-22
申请号：US10583301
申请日：2004-12-17
申请人： Salehuzzaman Shah , Randall Weselake
发明人： Salehuzzaman Shah , Randall Weselake
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8247
摘要： The present invention provides transgenic plants with reduced levels of saturated fatty acids in the seed oil and methods of making these plants. The transgenic plants developed through this method contain reduced levels of saturated fatty acids in seed oil due to expression of a prokaryotic delta-9 desaturase enzyme (i.e. an enzyme that introduces cis double bonds at the delta-9 position of saturated fatty acids) operably linked with an endoplasmic reticulum retention and retrieval signal sequence. One example of the invention is a plant expressing a heterologous delta-9 desaturase enzyme from cyanobacterium Anacystis nidulans, which converts lipid-bound 16:0 and 18:0 fatty acids into corresponding 16:1 and 18:1, in operative linkage with a KKSS (SEQ ID NO:5) endoplasmic reticulum retention and retrieval signal sequence.
摘要翻译：本发明提供种子油中饱和脂肪酸含量降低的转基因植物和制备这些植物的方法。通过这种方法开发的转基因植物由于原核的δ-9去饱和酶（即在饱和脂肪酸的δ-9位引入顺式双键的酶）的表达，在种子油中含有降低的饱和脂肪酸水平，可操作地连接具有内质网保持和检索信号序列。本发明的一个实例是表达来自构成蓝细菌构巢曲霉的异源δ-9去饱和酶的植物，其将脂质结合的16：0和18：0脂肪酸转化成相应的16：1和18：1，与 KKSS（SEQ ID NO：5）内质网保持和检索信号序列。

3. 发明申请

US20160298148A1 ENRICHMENT OF OILS WITH POLYUNSATURATED FATTY ACIDS 审中-公开
标题翻译：油脂与多糖脂肪酸的混合
公开(公告)号：US20160298148A1
公开(公告)日：2016-10-13
申请号：US14405439
申请日：2013-06-07
申请人： Randall WESELAKE , Xue PAN , Rodrigo SILOTO
发明人： Randall WESELAKE , Xue PAN , Rodrigo SILOTO
IPC分类号： C12P7/64 , C12N9/10
CPC分类号： C12P7/6472 , C12N9/1029 , C12N15/8247 , C12Y203/01158
摘要： The present invention relates to isolated phospholipid:diacylglycerol acyltransferases (PDAT) and polynucleotide sequences encoding the PDAT enzymes; polynucleotide constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same. Also provided are transformed cells and transgenic plants with enhanced oil accumulation and quality.
摘要翻译：本发明涉及分离的磷脂：二酰基甘油酰基转移酶（PDAT）和编码PDAT酶的多核苷酸序列; 多核苷酸构建体，载体和掺入多核苷酸序列的宿主细胞; 以及其制造和使用方法。还提供了具有增强的油积聚和质量的转化细胞和转基因植物。

4. 发明申请

US20110190165A1 HIGH THROUGHPUT METHODS OF IDENTIFYING NEUTRAL LIPID SYNTHASES 审中-公开
标题翻译：鉴定中性脂质合成的高通量方法
公开(公告)号：US20110190165A1
公开(公告)日：2011-08-04
申请号：US12993055
申请日：2009-05-19
申请人： Randall Weselake , Rodrigo Siloto , Martin Reusksa
发明人： Randall Weselake , Rodrigo Siloto , Martin Reusksa
IPC分类号： C40B30/08 , C12Q1/48
CPC分类号： C12N15/1034 , C12N9/1051 , G01N2333/91051
摘要： The present invention relates to high throughput methods of identifying neutral lipid synthases. The invention includes a method of positively selecting yeast cells expressing recombinant neutral lipid synthases, and quantifying the enzyme activities of the recombinant neutral lipid synthases using a fluorescence in situ assay.
摘要翻译：本发明涉及鉴定中性脂质合酶的高通量方法。本发明包括积极选择表达重组中性脂质合酶的酵母细胞的方法，并使用荧光原位测定法定量重组中性脂质合酶的酶活性。

5. 发明申请

US20080092254A1 Transgenic Plants with Reduced Level of Saturated Fatty Acid and Methods for Making Them 失效
标题翻译：具有降低饱和脂肪酸水平的转基因植物及其制备方法
公开(公告)号：US20080092254A1
公开(公告)日：2008-04-17
申请号：US10583301
申请日：2004-12-17
申请人： Salehuzzaman Shah , Randall Weselake
发明人： Salehuzzaman Shah , Randall Weselake
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N5/04 , C12N9/00
CPC分类号： C12N15/8247
摘要： The present invention provides transgenic plants with reduced levels of saturated fatty acids in the seed oil and methods of making these plants. The transgenic plants developed through this method contain reduced levels of saturated fatty acids in seed oil due to expression of a prokaryotic delta-9 desaturase enzyme (i.e. an enzyme that introduces cis double bonds at the delta-9 position of saturated fatty acids) operably linked with an endoplasmic reticulum retention and retrieval signal sequence. One example of the invention is a plant expressing a heterologous delta-9 desaturase enzyme from cyanobacterium Anacystis nidulans, which converts lipid-bound 16:0 and 18:0 fatty acids into corresponding 16:1 and 18:1, in operative linkage with a KKSS (SEQ ID NO:5) endoplasmic reticulum retention and retrieval signal sequence.
摘要翻译：本发明提供种子油中饱和脂肪酸含量降低的转基因植物和制备这些植物的方法。通过这种方法开发的转基因植物由于原核的δ-9去饱和酶（即在饱和脂肪酸的δ-9位引入顺式双键的酶）的表达，在种子油中含有降低的饱和脂肪酸水平，可操作地连接具有内质网保持和检索信号序列。本发明的一个实例是表达来自构成蓝细菌构巢曲霉的异源δ-9去饱和酶的植物，其将脂质结合的16：0和18：0脂肪酸转化成相应的16：1和18：1，与 KKSS（SEQ ID NO：5）内质网保持和检索信号序列。

6. 发明申请

US20100024078A1 Diacylglycerol acyltransferases from flax 审中-公开
标题翻译：来自亚麻的二酰基甘油酰基转移酶
公开(公告)号：US20100024078A1
公开(公告)日：2010-01-28
申请号：US12381212
申请日：2009-03-09
申请人： Randall Weselake , Rodrigo Siloto , Qin Liu , Andre Laroche
发明人： Randall Weselake , Rodrigo Siloto , Qin Liu , Andre Laroche
IPC分类号： A01H5/00 , C07H21/00 , C12N15/74 , C12N1/16
CPC分类号： C12P7/6463 , C12N9/1029 , C12N15/8247
摘要： The invention relates to isolated diacylglycerol acyltransferases and polynucleotide sequences encoding the DGAT enzymes; polynucleotide constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same. Also provided are transformed cells and transgenic plants, with enhanced oil accumulation and quality.
摘要翻译：本发明涉及分离的二酰基甘油酰基转移酶和编码DGAT酶的多核苷酸序列; 多核苷酸构建体，载体和掺入多核苷酸序列的宿主细胞; 以及其制造和使用方法。还提供了转化细胞和转基因植物，具有增强的油积聚和质量。

7. 发明申请

US20090249516A1 Diacylglycerol acyltransferases from flax 有权
标题翻译：来自亚麻的二酰基甘油酰基转移酶
公开(公告)号：US20090249516A1
公开(公告)日：2009-10-01
申请号：US12381183
申请日：2009-03-09
申请人： Randall Weselake , Rodrigo Siloto , Qin Liu , Andre Laroche , Eric Murphy , Kimmo Koivu
发明人： Randall Weselake , Rodrigo Siloto , Qin Liu , Andre Laroche , Eric Murphy , Kimmo Koivu
IPC分类号： A01H1/00 , C07H21/00 , C12N15/74 , C12N1/00 , A01H5/00 , A01H5/10 , C11B3/00
CPC分类号： C12P7/6463 , C12N9/1029 , C12N15/8247
摘要： The invention relates to isolated diacylglycerol acyltransferases and polynucleotide sequences encoding the DGAT enzymes; polynucleotide constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same. Also provided are transformed cells and transgenic plants, especially Camelina sativa plant, with enhanced oil accumulation and quality.
摘要翻译：本发明涉及分离的二酰基甘油酰基转移酶和编码DGAT酶的多核苷酸序列; 多核苷酸构建体，载体和掺入多核苷酸序列的宿主细胞; 以及其制造和使用方法。还提供转化细胞和转基因植物，特别是Camelina sativa植物，具有增强的油积聚和质量。

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