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1. 发明授权

US07364905B2 Apparatus and method for high throughput analysis of compound-membrane interactions 失效
标题翻译：化合物 - 膜相互作用的高通量分析的装置和方法
公开(公告)号：US07364905B2
公开(公告)日：2008-04-29
申请号：US10139766
申请日：2002-05-06
申请人： Ilya Feygin , Rafail Kushak
发明人： Ilya Feygin , Rafail Kushak
IPC分类号： G01N33/00
CPC分类号： B01J19/0046 , B01J2219/00286 , B01J2219/00423 , B01J2219/00585 , B01J2219/00599 , B01J2219/00756 , C40B60/12 , Y10T436/10
摘要： An article and method for high throughput and a high content investigation of compound interactions with live tissue or its substitutes under controlled conditions during compound absorption and related processes. In some variations of the illustrative embodiment, the article is a multi-chamber enclosure having at least two chambers separated by a membrane. Membranes can be prepared from live epithelial tissue or from an artificial material with or without attached cells from cell-line cultures. Each chamber is advantageously connected to a fluidic-control system by tubes that pass through a feed fitting. In addition to coupling the chambers with the fluidic-control system, the feed fitting, which is spring-biased, provides a sealing force to seal the enclosure. In some variations, one or more multi-chamber enclosures are installed in a mother chamber, which provides controlled environmental conditions. Operation of the article includes automatic introduction of compounds, buffers, and gases into the chambers, establishing reaction conditions inside the chambers, and individually sampling from the chambers.
摘要翻译：一种用于在化合物吸收和相关过程中在受控条件下与活组织或其替代物的复合相互作用的高通量和高含量的研究的文章和方法。在说明性实施例的一些变型中，制品是具有由隔膜隔开的至少两个室的多室外壳。膜可以从活上皮组织或具有或不具有来自细胞系培养物的附着细胞的人造材料制备。每个室有利地通过穿过进料配件的管连接到流体控制系统。除了将腔室与流体控制系统联接之外，被弹簧偏置的进给配件提供密封力以密封外壳。在一些变型中，一个或多个多腔室安装在母室中，其提供受控的环境条件。物品的操作包括将化合物，缓冲剂和气体自动引入室中，在室内建立反应条件，并从室中单独取样。

2. 发明授权

US07179637B2 Apparatus and method for using intestinal segments or their substitutes in high-throughput analysis of compound absorption 失效
标题翻译：在化合物吸收的高通量分析中使用肠段或其替代物的装置和方法
公开(公告)号：US07179637B2
公开(公告)日：2007-02-20
申请号：US10225057
申请日：2002-08-21
申请人： Ilya Feygin , Rafail Kushak
发明人： Ilya Feygin , Rafail Kushak
IPC分类号： C12M3/00
CPC分类号： B01J19/0046 , B01J2219/00286 , B01J2219/00423 , B01J2219/00585 , B01J2219/00599 , B01J2219/00756 , Y10S623/915 , Y10S623/917
摘要： An apparatus for high-throughput analysis of compound absorption in a controlled environment that closely resembles in-vivo conditions. The apparatus includes at least one tissue-receiving enclosure that is capable of interfacing (e.g., via transfer of fluids, electronically, etc.) with a variety of preparative devices and analytical instrumentation. In some variations, the tissue-receiving enclosure is formed from two housings, which, when engaged, have a capsular shape. The enclosure receives a tissue sample in the form of an un-everted or everted intestinal segment or tubular artificial membrane. The tissue sample is coupled to a generally cylindrical tissue support. The tissue support and the enclosure are axially aligned, and openings at the ends of the enclosure align with openings at the ends of the tissue support. Two feed fittings, one for each end of the enclosure, forcefully mate with the aligned openings in the housing and the tissue support. The feed fitting seals against the ends of the tissue support, forming “outer” and “inner” chambers within the enclosure, with the intestinal tissue being the interface between the chambers. It is across this interface that absorption occurs.
摘要翻译：用于在与体内条件非常相似的受控环境中高通量分析化合物吸收的装置。该装置包括至少一个能够与各种制备装置和分析仪器进行接口（例如通过流体，电子传递等）接触的组织接收外壳。在一些变型中，组织接收外壳由两个外壳形成，当外壳接合时，其具有荚膜形状。外壳接收非直肠或直肠肠段或管状人造膜形式的组织样本。组织样本联接到大致圆柱形的组织支撑。组织支撑件和外壳被轴向对齐，并且外壳端部处的开口与组织支撑件端部处的开口对齐。两个进给配件，一个用于外壳的每一端，与壳体和组织支撑件中对齐的开口强制配合。进料配件密封组织支撑件的端部，在外壳内形成“外”和“内”腔，肠组织是腔室之间的界面。在这个界面上，吸收发生。

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