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1. 发明申请

WO2012016222A2 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 审中-公开
标题翻译：修改农杆菌菌株以增加植物转化频率
公开(公告)号：WO2012016222A2
公开(公告)日：2012-02-02
申请号：PCT/US2011/046028
申请日：2011-07-29
申请人： DOW AGROSCIENCES LLC , MERLO, Donald J. , RUSSELL, Sean M. , RETALLACK, Diane , WOOSLEY, Aaron , MEADE, Tom , NARVA, Kenneth E.
发明人： MERLO, Donald J. , RUSSELL, Sean M. , RETALLACK, Diane , WOOSLEY, Aaron , MEADE, Tom , NARVA, Kenneth E.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/84 , C12N1/21
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体，Ti质粒和植物转化二元载体进行复制的质粒上携带转化增强基因的土壤杆菌菌株，以及这些农杆菌菌株的用途。另外，缺乏DNA重组功能的农杆菌菌株导致植物转化双向载体的不稳定或重排，并且能够独立于农杆菌染色体，Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因还提供了这些菌株的载体和用途。还包括农杆菌菌株，其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。还描述了使用这些农杆菌菌株制备的植物。

2. 发明申请

WO2008070845A2 NOVEL SELECTABLE MARKER GENES 审中-公开
标题翻译：新的可选标记基因
公开(公告)号：WO2008070845A2
公开(公告)日：2008-06-12
申请号：PCT/US2007086813
申请日：2007-12-07
申请人： DOW AGROSCIENCES LLC , LIRA JUSTIN M , WRIGHT TERRY R , RUSSELL SEAN M , MERLO DONALD J , WEBB STEVEN ROBERT , ARNOLD NICOLE L , ROBINSON ANDREW E , SMITH KELLEY A
发明人： LIRA JUSTIN M , WRIGHT TERRY R , RUSSELL SEAN M , MERLO DONALD J , WEBB STEVEN ROBERT , ARNOLD NICOLE L , ROBINSON ANDREW E , SMITH KELLEY A
IPC分类号： A01H5/00
CPC分类号： C12N15/821 , C12N15/8275
摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.
摘要翻译：本发明涉及在本文中称为DSM-2的新基因。该基因在天蓝色链霉菌A3中鉴定。 DSM-2蛋白与PAT和BAR远缘相关。本发明还提供了编码DSM-2蛋白的植物优化的基因，DSM-2可以用作转基因性状以赋予植物和植物细胞对除草剂草丁膦和双丙氨膦的耐受性。主题基因的一个优选用途是作为选择标记。将该基因用作细菌系统中的选择标记可以提高植物转化的效率。使用DSM-2作为唯一选择标记消除了在克隆期间需要额外的医学抗生素标记（例如氨苄青霉素抗性）。根据本发明，各种其他用途也是可能的。

3. 发明申请

WO2007149715A2 NOVEL DNA SEQUENCES, VECTORS AND PROTEINS OF AVIAN INFLUENZA HEMAGGLUTININ 审中-公开
标题翻译： AVIAN INFLUENZA HEMAGGLUTININ的新型DNA序列，载体和蛋白
公开(公告)号：WO2007149715A2
公开(公告)日：2007-12-27
申请号：PCT/US2007/070755
申请日：2007-06-08
申请人： DOW AGROSCIENCES LLC , HENRY, Matthew , LARRINUA, Ignacio, M. , RUSSELL, Sean, M.
发明人： HENRY, Matthew , LARRINUA, Ignacio, M. , RUSSELL, Sean, M.
IPC分类号： A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/545 , C07K14/005 , C07K2319/00 , C12N15/8258 , C12N2760/16122 , C12N2760/16134
摘要： The subject invention provides novel amino acid sequences (including a consensus sequence) of the Avian Influenza A virus hemagglutinin protein. These newly constructed genes are designed to provide a broader spectrum of activity across the serotype family thus providing a basis for a vaccine that has broad heterologous disease protection. The novel genes have been further improved by the addition of strategic glycosylation sites into the amino acids sequences that they encode. These genes can also, optionally, be codon optimized for plant expression, inserted into the appropriate vector and cloned into plants for expression. Polypeptides produced by recombinant host cells or transgenic plants can also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals. Additionally, transgenic plant material may also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals.
摘要翻译：本发明提供禽流感A病毒血凝素蛋白的新型氨基酸序列（包括共有序列）。这些新构建的基因被设计为提供跨血清型家族的更广泛的活性范围，从而为具有广泛异源疾病保护的疫苗提供基础。通过在其编码的氨基酸序列中加入战略糖基化位点，进一步改善了这些新基因。这些基因也可任选地优化用于植物表达的密码子，插入合适的载体中并克隆到植物中用于表达。通过重组宿主细胞或转基因植物产生的多肽也可以用作疫苗来源，用于制定用于控制易感个体中的流感的疫苗。另外，转基因植物材料也可以用作疫苗的来源，用于制定用于控制易感个体中的流感的疫苗。

4. 发明申请

WO2005067491A2 TOXIN COMPLEX PROTEINS AND GENES FROM XENORHABDUS BOVIENII 审中-公开
标题翻译：毒素复合蛋白和XENORHABDUS BOVIENII的基因
公开(公告)号：WO2005067491A2
公开(公告)日：2005-07-28
申请号：PCT/US2004/043000
申请日：2004-12-23
申请人： DOW AGROSCIENCES LLC , APEL-BIRKHOLD, Patricia, C. , HEY, Timothy, D. , SHEETS, Joel, J. , MEADE, Thomas , LI, Ze, Sheng , LIRA, Justin, M. , RUSSELL, Sean, M. , THOMPSON, Robin, L. , MITCHELL, Jon, C. , FENCIL, Kristin
发明人： APEL-BIRKHOLD, Patricia, C. , HEY, Timothy, D. , SHEETS, Joel, J. , MEADE, Thomas , LI, Ze, Sheng , LIRA, Justin, M. , RUSSELL, Sean, M. , THOMPSON, Robin, L. , MITCHELL, Jon, C. , FENCIL, Kristin
IPC分类号： A01H1/00 , A01H5/00 , A01N63/00 , A01N63/02 , C07H21/04 , C07K14/24 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07H21/04 , C07K14/24 , C12N15/8279 , Y02A40/162 , Y10S530/825
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus bovienii strain ILM104.
摘要翻译：本发明涉及新型异戊酰梭菌毒素复合物（TC）蛋白质和编码这些蛋白质的基因。更具体地，本发明涉及从Xenorhabdus bovienii菌株ILM104获得的TC基因和蛋白质。

5. 发明申请

WO2012016222A3 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 审中-公开
标题翻译：农杆菌菌株改良以提高植物转化频率
公开(公告)号：WO2012016222A3
公开(公告)日：2012-06-21
申请号：PCT/US2011046028
申请日：2011-07-29
申请人： DOW AGROSCIENCES LLC , MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
发明人： MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
IPC分类号： A01H5/00 , C12N1/21 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体复制的质粒上携带转化增强基因的农杆菌菌株，Ti质粒和植物转化双元载体，以及这些农杆菌菌株的用途。此外，导致植物转化双元载体不稳定或重排的DNA重组功能缺陷的农杆菌菌株，以及在能够独立于农杆菌染色体，Ti质粒和植物转化二元体复制的质粒上携带转化增强基因载体，以及这些菌株的用途。进一步包括的是农杆菌菌株，其具有整合到农杆菌染色体中的转化增强基因，其不会干扰土壤杆菌细胞的正常生长和植物转化能力或者以其它方式危害土壤杆菌细菌的植物转化能力，以及这些农杆菌菌株的用途。还描述了使用这些农杆菌菌株制造的植物。

6. 发明申请

WO2008070845A3 NOVEL SELECTABLE MARKER GENES 审中-公开
公开(公告)号：WO2008070845A3
公开(公告)日：2008-06-12
申请号：PCT/US2007/086813
申请日：2007-12-07
申请人： DOW AGROSCIENCES LLC , LIRA, Justin, M. , WRIGHT, Terry, R. , RUSSELL, Sean, M. , MERLO, Donald, J. , WEBB, Steven, Robert , ARNOLD, Nicole, L. , ROBINSON, Andrew, E. , SMITH, Kelley, A.
发明人： LIRA, Justin, M. , WRIGHT, Terry, R. , RUSSELL, Sean, M. , MERLO, Donald, J. , WEBB, Steven, Robert , ARNOLD, Nicole, L. , ROBINSON, Andrew, E. , SMITH, Kelley, A.
IPC分类号： C12N1/21 , C12N15/82 , A01H1/00
摘要： The subject invention relates to a novel gene referred to herein as DSM-2 . This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

7. 发明申请

WO2007149715A3 NOVEL DNA SEQUENCES, VECTORS AND PROTEINS OF AVIAN INFLUENZA HEMAGGLUTININ 审中-公开
标题翻译： AVIAN INFLUENZA HEMAGGLUTININ的新型DNA序列，载体和蛋白
公开(公告)号：WO2007149715A3
公开(公告)日：2008-07-24
申请号：PCT/US2007070755
申请日：2007-06-08
申请人： DOW AGROSCIENCES LLC , HENRY MATTHEW , LARRINUA IGNACIO M , RUSSELL SEAN M
发明人： HENRY MATTHEW , LARRINUA IGNACIO M , RUSSELL SEAN M
IPC分类号： A61K39/12 , A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/545 , C07K14/005 , C07K2319/00 , C12N15/8258 , C12N2760/16122 , C12N2760/16134
摘要： The subject invention provides novel amino acid sequences (including a consensus sequence) of the Avian Influenza A virus hemagglutinin protein. These newly constructed genes are designed to provide a broader spectrum of activity across the serotype family thus providing a basis for a vaccine that has broad heterologous disease protection. The novel genes have been further improved by the addition of strategic glycosylation sites into the amino acids sequences that they encode. These genes can also, optionally, be codon optimized for plant expression, inserted into the appropriate vector and cloned into plants for expression. Polypeptides produced by recombinant host cells or transgenic plants can also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals. Additionally, transgenic plant material may also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals.
摘要翻译：本发明提供禽流感A病毒血凝素蛋白的新型氨基酸序列（包括共有序列）。这些新构建的基因被设计为提供跨血清型家族的更广泛的活性范围，从而为具有广泛异源疾病保护的疫苗提供基础。通过在其编码的氨基酸序列中加入战略糖基化位点，进一步改善了这些新基因。这些基因也可任选地优化用于植物表达的密码子，插入合适的载体中并克隆到植物中用于表达。通过重组宿主细胞或转基因植物产生的多肽也可以用作疫苗来源，用于制定用于控制易感个体中的流感的疫苗。另外，转基因植物材料也可以用作疫苗的来源，用于制定用于控制易感个体中的流感的疫苗。

8. 发明申请

WO2005067491A3 TOXIN COMPLEX PROTEINS AND GENES FROM XENORHABDUS BOVIENII 审中-公开
标题翻译：毒素复合蛋白和XENORHABDUS BOVIENII的基因
公开(公告)号：WO2005067491A3
公开(公告)日：2006-05-18
申请号：PCT/US2004043000
申请日：2004-12-23
申请人： DOW AGROSCIENCES LLC , APEL-BIRKHOLD PATRICIA C , HEY TIMOTHY D , SHEETS JOEL J , MEADE THOMAS , LI ZE SHENG , LIRA JUSTIN M , RUSSELL SEAN M , THOMPSON ROBIN L , MITCHELL JON C , FENCIL KRISTIN
发明人： APEL-BIRKHOLD PATRICIA C , HEY TIMOTHY D , SHEETS JOEL J , MEADE THOMAS , LI ZE SHENG , LIRA JUSTIN M , RUSSELL SEAN M , THOMPSON ROBIN L , MITCHELL JON C , FENCIL KRISTIN
IPC分类号： C12N15/31 , A01H1/00 , A01H5/00 , A01N63/00 , A01N63/02 , C07H21/04 , C07K14/24 , C12N5/04 , C12N15/00 , C12N15/03 , C12N15/05 , C12N15/08 , C12N15/10 , C12N15/32 , C12N15/82 , C12P21/02
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07H21/04 , C07K14/24 , C12N15/8279 , Y02A40/162 , Y10S530/825
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus bovienii strain ILM104.
摘要翻译：本发明涉及新型异戊酰梭菌毒素复合物（TC）蛋白质和编码这些蛋白质的基因。更具体地，本发明涉及从Xenorhabdus bovienii菌株ILM104获得的TC基因和蛋白质。

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