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1. 发明申请

WO2006056480A2 MEANS AND METHODS FOR DETECTING METHYLATED DNA 审中-公开
标题翻译：检测甲基化DNA的方法和方法
公开(公告)号：WO2006056480A2
公开(公告)日：2006-06-01
申请号：PCT/EP2005/012707
申请日：2005-11-28
申请人： KLINIKUM DER UNIVERSITÄT REGENSBURG , REHLI, Michael
发明人： REHLI, Michael
CPC分类号： C12Q1/6886 , C07K14/4703 , C07K2317/52 , C07K2319/00 , C07K2319/20 , C07K2319/30 , C07K2319/80 , C12Q2537/164
摘要： The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
摘要翻译：本申请涉及具有编码双功能多肽的核苷酸序列的核酸分子，其包含属于甲基-CpG结合蛋白（MBD）家族的蛋白质和抗体的Fc部分的DNA结合结构域。此外，公开了包含所述核酸分子和由所述核酸分子编码的多肽的载体和宿主细胞以及产生所述多肽的方法。此外，本申请提供了特异性结合所述多肽和组合物的抗体，特别是包含核酸分子，载体，宿主细胞，多肽或抗体的诊断组合物现在申请。此外，提供了使用本发明的多肽检测甲基化DNA，特别是在肿瘤组织或肿瘤细胞中的方法和用途。

2. 发明申请

WO2006056480A3 MEANS AND METHODS FOR DETECTING METHYLATED DNA 审中-公开
标题翻译：检测甲基化DNA的方法和方法
公开(公告)号：WO2006056480A3
公开(公告)日：2008-03-27
申请号：PCT/EP2005012707
申请日：2005-11-28
申请人： UNIV REGENSBURG KLINIKUM , REHLI MICHAEL
发明人： REHLI MICHAEL
IPC分类号： C12Q1/68 , C07K14/47 , C12N15/10 , C12N15/62 , G01N33/574
CPC分类号： C12Q1/6886 , C07K14/4703 , C07K2317/52 , C07K2319/00 , C07K2319/20 , C07K2319/30 , C07K2319/80 , C12Q2537/164
摘要： The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
摘要翻译：本申请涉及具有编码双功能多肽的核苷酸序列的核酸分子，其包含属于甲基-CpG结合蛋白（MBD）家族的蛋白质和抗体的Fc部分的DNA结合结构域。此外，公开了包含所述核酸分子和由所述核酸分子编码的多肽的载体和宿主细胞以及产生所述多肽的方法。此外，本申请提供了特异性结合所述多肽和组合物的抗体，特别是包含核酸分子，载体，宿主细胞，多肽或抗体的诊断组合物现在申请。此外，提供了使用本发明的多肽检测甲基化DNA，特别是在肿瘤组织或肿瘤细胞中的方法和用途。

3. 发明申请

WO2006056478A1 KITS AND METHODS FOR DETECTING METHYLATED DNA 审中-公开
标题翻译：用于检测甲基化DNA的试剂盒和方法
公开(公告)号：WO2006056478A1
公开(公告)日：2006-06-01
申请号：PCT/EP2005/012705
申请日：2005-11-28
申请人： KLINIKUM DER UNIVERSITÄT REGENSBURG , REHLI, Michael
发明人： REHLI, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6886 , C12Q2600/154 , C12Q2600/16
摘要： The present invention relates to an in vitro method for detecting methylated DNA comprising (a) coating a container with a polypeptide capable of binding methylated DNA; (b) contacting said polypeptide with a sample comprising methylated and/or unmethylated DNA; and (c) detecting the binding of said polypeptide to methylated DNA. In a preferred embodiment, said method further comprises step (d) analyzing the detected methylated DNA by sequencing. Another aspect of the present invention is a kit for detecting methylated DNA according to the methods of the invention comprising (a) a polypeptide capable of binding methylated DNA; (b) a container which can be coated with said polypeptide; (c) means for coating said container; and (d) means for detecting methylated DNA.
摘要翻译：本发明涉及检测甲基化DNA的体外方法，其包括（a）用能够结合甲基化DNA的多肽涂覆容器; （b）使所述多肽与包含甲基化和/或未甲基化的DNA的样品接触; 和（c）检测所述多肽与甲基化DNA的结合。在优选的实施方案中，所述方法还包括步骤（d）通过测序分析所检测的甲基化DNA。本发明的另一方面是根据本发明的方法检测甲基化DNA的试剂盒，其包含（a）能够结合甲基化DNA的多肽; （b）可以涂覆有所述多肽的容器; （c）用于涂覆所述容器的装置; 和（d）检测甲基化DNA的方法。

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