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    • 1. 发明申请
    • TIP ELECTRODE CHAMBER FOR SMALL VOLUME ELECTROPORATION
    • TIP电极室用于小容量电气
    • WO2008051169A1
    • 2008-05-02
    • PCT/SI2007/000036
    • 2007-10-03
    • UNIVERZA V LJUBLJANI, FAKULTETA ZA ELEKTROTEHNIKOTRONTELJ, KatjaREBERSEK, MatejMIKLAVCIC, Damjan
    • TRONTELJ, KatjaREBERSEK, MatejMIKLAVCIC, Damjan
    • C12M3/00C12M1/42
    • C12M35/02
    • Subject of present invention is an apparatus for electroporation with pipetter, multi-electrodes design with minimized inhomogeneous electric field and solid filler for electrode chambers to minimize inhomogeneous electric field. A tip electrode chamber for electroporation according to the invention is characterized in that the opening (5) is in the middle of housing (1) and in the longitudinal direction through the housing (1) and that from two to six electrodes (2) are parallelly and symmetrically mounted into the inner surface of the housing (1) and longitudinally to the opening (5) and partially extending to the said opening (5) and that the said electrodes (2) have electrical connections (3) to exterior. A lso a tip electrode chamber is disclosed, where the solid filler (6) is inserted into the said opening (5) with the hole (7) in the longitudinal direction of the housing 1 and solid filler (6) two to six electrodes (2) are parallelly and symmetrically mounted into the contact of surfaces of the housing (1) and solid filler (6) and that the said electrodes (2) have electrical connections (3) to exterior.
    • 本发明的主题是一种使用移液器进行电穿孔的设备,具有最小不均匀电场的多电极设计和用于电极室的固体填料以最小化不均匀的电场。 根据本发明的用于电穿孔的尖端电极室的特征在于,开口(5)在壳体(1)的中间,并且在纵向方向上穿过壳体(1),并且从两个到六个电极(2) 平行和对称地安装到壳体(1)的内表面中并纵向延伸到开口(5)并且部分地延伸到所述开口(5),并且所述电极(2)具有到外部的电连接(3)。 公开了一种尖端电极室,其中固体填料(6)插入到所述开口(5)中,孔(7)沿壳体1的纵向方向插入孔中,固体填料(6)插入两至六个电极 2)平行和对称地安装到壳体(1)和固体填充物(6)的表面的接触中,并且所述电极(2)具有到外部的电连接(3)。