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1. 发明授权

US6124129A Compositions and methods for controlling genetically engineered organisms 失效
标题翻译：用于控制转基因生物的组合物和方法
公开(公告)号：US6124129A
公开(公告)日：2000-09-26
申请号：US19966
申请日：1998-02-06
申请人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
发明人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
IPC分类号： C07K14/36 , C12N1/21 , C12N15/72 , C12N15/74 , C12N1/20
CPC分类号： C07K14/36 , C12N15/72 , C12N15/74
摘要： Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.
摘要翻译：描述了用于控制转基因生物的组合物和方法。基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。

2. 发明授权

US5679533A Biotin-binding containment systems 失效
公开(公告)号：US5679533A
公开(公告)日：1997-10-21
申请号：US479390
申请日：1995-06-07
申请人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
发明人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
IPC分类号： C07K14/36 , C07K14/465 , C12N1/21 , C12N15/12 , C12N15/70 , G01N33/53
CPC分类号： C12N15/70 , C07K14/36 , C07K14/465
摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

3. 发明授权

US06287844B1 Compositions and methods for controlling genetically engineered organisms 失效
标题翻译：用于控制转基因生物的组合物和方法
公开(公告)号：US06287844B1
公开(公告)日：2001-09-11
申请号：US09019966
申请日：1998-02-06
申请人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
发明人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
IPC分类号： C12N120
CPC分类号： C07K14/36 , C12N15/72 , C12N15/74
摘要： Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.
摘要翻译：描述了用于控制转基因生物的组合物和方法。基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。

4. 发明授权

US5753439A Nucleic acid detection methods 失效
标题翻译：核酸检测方法
公开(公告)号：US5753439A
公开(公告)日：1998-05-19
申请号：US446102
申请日：1995-05-19
申请人： Cassandra L. Smith , Ron Yaar , Przemyslaw Szafranski , Charles R. Cantor
发明人： Cassandra L. Smith , Ron Yaar , Przemyslaw Szafranski , Charles R. Cantor
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6827 , C12Q1/6823
摘要： The invention relates to methods for rapidly determining the sequence and/or length a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguish from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.
摘要翻译：本发明涉及用于快速确定靶序列的序列和/或长度的方法。靶序列可以是与探针阵列杂交的一系列已知或未知的重复序列。杂交阵列用单链核酸酶消化，游离的3'-羟基用核酸聚合酶延伸。核酸酶切割的异源双链体可以通过差异标记容易地与核酸酶未切割的异源双链体区分开。探针和靶可以用可检测的标记差异标记。可以通过从杂交靶标切割得到的环并产生游离的3-羟基来检测匹配的靶。这些基团被加入到反应体系中的聚合酶识别和扩增，该反应体系也将一个标记物添加或释放到溶液中。使用固相或溶液分析所得产物。这些方法可用于检测特征性核酸序列，确定靶序列并筛选遗传缺陷和病症。测定可以在固体表面进行，允许并行进行多个反应，如果需要，可以自动进行。

5. 发明授权

US5681745A Biotin-binding containment systems 失效
标题翻译：生物素结合遏制系统
公开(公告)号：US5681745A
公开(公告)日：1997-10-28
申请号：US432017
申请日：1995-05-01
申请人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
发明人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
IPC分类号： C07K14/36 , C07K14/465 , C12N1/21 , C12N15/12 , C12N15/70 , C12N5/10 , C12N1/13
CPC分类号： C12N15/70 , C07K14/36 , C07K14/465
摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.
摘要翻译：本发明涉及表达可用于选择性破坏重组细胞如遗传工程微生物的生物素结合成分的遗传容纳系统。这些系统可以包含链霉亲和素或抗生物素蛋白基因，其表达受可调节启动子控制。诸如转录效应子的调节剂由另外可能被表达的基因表达，其表达由遏制系统控制。试剂的表达可以设计成响应环境中的生理变化。本发明还涉及用于选择性检测或跟踪重组细胞和含有这些遗传包涵体系的真核和原核细胞的遏制系统和方法。

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