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1. 发明申请

US20100172938A1 MODIFIED BACTERIAL SURFACE LAYER PROTEINS 审中-公开
标题翻译：改性细菌表面层蛋白
公开(公告)号：US20100172938A1
公开(公告)日：2010-07-08
申请号：US12648271
申请日：2009-12-28
申请人： Pieter Hendrik Pouwels , Egbert Smit , Frans Tielen
发明人： Pieter Hendrik Pouwels , Egbert Smit , Frans Tielen
IPC分类号： A61K39/02 , C07K14/195 , C07K14/335 , C07K7/08 , C07H21/00 , C12N15/74 , C12N1/21 , C40B40/10 , C12P21/00 , A61P31/04 , A61P37/04
CPC分类号： C07K14/335 , C07K2319/00 , C12N2810/55
摘要： Modified bacterial surface layer (S-layer) proteins are disclosed where the modification is the insertion, at an internal location, of a heterologous polypeptide, or polypeptide of interest. The polypeptide is a binding or target protein, such as an antigen or antibody, or part thereof, in particular a bacterial antigen (e.g. from Clostridium tetani such as TTFC). The modified surface layer protein can then be expressed on the surface of the bacterial cell and used in a vaccine. Also disclosed are bacteria which have been modified to express a heterologous surface layer protein, but which do not as a wild-type possess an S-layer (such as L. casei), and modified bacteria which express only a modified surface layer protein (and not the wild-type S-layer protein). The wild type S-layer is completely replaced with the modified version where the polynucleotide encoding the modified version is integrated into the bacterial genome. The modified S-proteins can form crystalline arrays, sheets or layers that can be used to bind functional molecules (e.g. receptors) to solid surfaces (Au, silicon wafers) in biosensors.
摘要翻译：公开了修饰的细菌表面层（S层）蛋白，其中修饰是在异源多肽或感兴趣的多肽的内部位置插入。多肽是结合或靶蛋白，例如抗原或抗体，或其部分，特别是细菌抗原（例如来自破伤风梭菌如TTFC）。然后可以将修饰的表面蛋白质在细菌细胞的表面上表达并用于疫苗中。还公开了已被修饰以表达异源表面层蛋白但不具有野生型的具有S层（例如干酪乳杆菌）的细菌和仅表达改性表面蛋白的修饰细菌（而不是野生型S层蛋白）。野生型S层完全被编码修饰版本的多核苷酸整合到细菌基因组中的修饰版本替代。修饰的S-蛋白质可以形成可用于将功能性分子（例如受体）结合到生物传感器中的固体表面（Au，硅晶片）的晶体阵列，片或层。

2. 发明授权

US06830901B1 Propionibacteruim vector 失效
标题翻译：丙酸杆菌载体
公开(公告)号：US06830901B1
公开(公告)日：2004-12-14
申请号：US09720583
申请日：2001-06-25
申请人： Pieter Hendrik Pouwels , Nicole Van Luijk , Johannes P. M. Jore , Rudolf G. M. Luiten
发明人： Pieter Hendrik Pouwels , Nicole Van Luijk , Johannes P. M. Jore , Rudolf G. M. Luiten
IPC分类号： C12P1942
CPC分类号： C12N15/746
摘要： An endogenous plasmid of Propionibacterium is described, isolated from Propionibacteria freudenreichii LMG 16545 (deposited as CBS 101022), and its sequence provided. This plasmid can be used to transform Propionibacteria to express homologous or heterologous proteins, in the production of recombinant proteins or products of enzymes, for example vitamin B12.
摘要翻译：描述了丙酸杆菌的内源性质粒，分离自丙酸杆菌属（Propionibacteria freudenreichii）LMG 16545（以CBS 101022保藏），并提供其序列。该质粒可用于转化丙酸杆菌以表达同源或异源蛋白质，在重组蛋白质或酶产物（例如维生素B12）的生产中。

3. 发明授权

US06506389B2 ADHERENCE FACTORS OF NON PATHOGENIC MICROORGANISMS AND APPLICATIONS THEREOF FOR SCREENING MICROORGANISMS FOR SPECIFIC PROBIOTIC PROPERTIES; NOVEL PHARMACEUTICAL COMPOSITIONS AND FOOD ADDITIVES COMPRISING SUCH MICROORGANISMS AND ADHERENCE FACTORS 失效
标题翻译：非致病微生物的辅助因子及其筛选特异性生物特性微生物的应用; 新型药物组合物和包含这些微生物和干扰因子的食品添加剂
公开(公告)号：US06506389B2
公开(公告)日：2003-01-14
申请号：US09051755
申请日：1998-09-30
申请人： Robert Jan Leer , Pieter Hendrik Pouwels , Patricia Lynne Conway
发明人： Robert Jan Leer , Pieter Hendrik Pouwels , Patricia Lynne Conway
IPC分类号： A61K3907
CPC分类号： C07K14/335 , A61K38/00 , Y02A50/47
摘要： A protein obtainable from a non pathgenic microorgansim, said protein having mucosa binding promoting activity and a molecular weight of 20-40 kD is disclosed. Application of such a protein or a peptide derived therefrom in a method of screening non pathogenic microorganisms for a microorganism capable of specifically binding mucosa, said method comprising detection in a manner known per se of the presence of a particular protein on or in a microorganism or in a culture of microorganisms, said particular protein being the already defined protein. Kits suitable for such a screening method are also disclosed. Use of a component selected from the group of components comprising a protein or peptide as defined; an expression vector comprising nucleic acid encoding such protein or peptide; a recombinant microorganism or a part of said microorganism expressing such protein or peptide, said part expressing mucosa binding promoting activity; a non pathogenic microorganism capable of expressing such protein or peptide or a part of said microorganism, said part expressing mucosa binding promoting activity as pharmaceutically active component in a pharmaceutical composition for prophylaxis and/or treatment of disease or illness associated with a mucosa colonizing pathogenic microorganism. Use of such components as food additive and compositions comprising such components are described.
摘要翻译：可从非致病微生物获得的蛋白质，所述蛋白质具有粘膜结合促进活性和20-40kD的分子量。将这种蛋白质或其衍生的肽应用于筛选非特异性结合粘膜的微生物的非病原微生物的方法中，所述方法包括以本身已知的方式检测特定蛋白质在微生物上或微生物中的存在，或在微生物培养物中，所述特定蛋白质是已经定义的蛋白质。还公开了适用于这种筛选方法的试剂盒。使用选自包含定义的蛋白质或肽的组分组的组分; 包含编码这种蛋白质或肽的核酸的表达载体; 表达所述蛋白质或肽的重组微生物或所述微生物的一部分，所述部分表达粘膜结合促进活性; 能够表达这种蛋白质或肽或一部分所述微生物的非致病微生物，所述部分表达作为药物活性成分的粘膜结合促进活性，用于预防和/或治疗与粘膜定植病原微生物相关的疾病或疾病的药物组合物。描述了使用这些组分作为食品添加剂和包含这些组分的组合物。

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