专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06444438B1 Method for the preparation of a protein by yeasts using an inducible system, vectors and correspondence transformed strains 失效
标题翻译：使用诱导系统，载体和对应转化菌株的酵母制备蛋白质的方法
公开(公告)号：US06444438B1
公开(公告)日：2002-09-03
申请号：US08453998
申请日：1995-05-30
申请人： Pierre H. Chambon , Daniel Metzger , John White
发明人： Pierre H. Chambon , Daniel Metzger , John White
IPC分类号： C12P2156
CPC分类号： C12N15/81 , C07K14/721
摘要： The present invention relates to a method for the preparation of a protein by yeasts. In particular, the method of the invention concerns transforming a yeast cell with a first DNA fragment encoding the protein and a second DNA fragment that encodes the receptor. The method further entails culturing the transformed yeast cell, and isolating the protein. The first DNA fragment is under control of elements providing for expression of the DNA fragment in yeast, which elements include a higher eukaryotic positive transcription control sequence consisting of a natural ligand responsive element activating sequence or a variant. The receptor is a natural nuclear receptor selected from the group consisting of receptors for steroids or for retinoids or for thyroid hormones or for vitamin D3. The receptor includes a first fragment that recognizes said ligand and a second fragment that binds to said transcriptional control sequence.
摘要翻译：本发明涉及通过酵母制备蛋白质的方法。特别地，本发明的方法涉及用编码该蛋白质的第一DNA片段和编码该受体的第二DNA片段转化酵母细胞。该方法还需要培养转化的酵母细胞，并分离蛋白质。第一个DNA片段在提供酵母中表达DNA片段的元件的控制下，该元件包括由天然配体反应元件激活序列或变体组成的高级真核阳性转录调控序列。受体是选自类固醇或类维生素A或受体甲状腺激素或维生素D3的受体的天然核受体。受体包括识别所述配体的第一片段和结合所述转录控制序列的第二片段。

2. 发明授权

US5817503A Method for the preparation of a protein by yeasts using an inducible system, vectors and corresponding transformed strains 失效
标题翻译：使用诱导系统，载体和相应的转化菌株通过酵母制备蛋白质的方法
公开(公告)号：US5817503A
公开(公告)日：1998-10-06
申请号：US453888
申请日：1995-05-30
申请人： Pierre H. Chambon , Daniel Metzger , John White
发明人： Pierre H. Chambon , Daniel Metzger , John White
IPC分类号： C12N1/19 , C07K14/72 , C12N15/09 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/865
CPC分类号： C12N15/81 , C07K14/721
摘要： The present invention relates to a method for the preparation of a protein by yeasts, according to which: yeast cells which contain the following are cultured: a DNA sequence coding for the protein under the control of elements providing for its expression in yeasts, the elements comprising a transcription control sequence which is inducible by a complex formed by a receptor and a ligand, a DNA sequence which is functional in yeast, coding for the receptor, the receptor comprising two essential portions, one of which recognizes the ligand so as to form a complex with the ligand and the other binds to the said transcription control sequence; the portion of the receptor which recognizes the ligand is of higher eukaryotic origin, and the ligand is not necessary for the culturing of the cells but is capable of entering the cells when added to the culture medium, the ligand is added to the culture medium at an appropriate time point for induction, the synthesized protein is recovered.
摘要翻译：本发明涉及一种通过酵母制备蛋白质的方法，其中：培养含有以下物质的酵母细胞：编码在酵母中提供其表达的元件控制下的蛋白质的DNA序列，元件包括由受体和配体形成的复合物可诱导的转录调控序列，编码受体的酵母中功能的DNA序列，受体包含两个必需部分，其中之一识别配体以形成与配体的复合物和另一个与所述转录控制序列结合; 识别配体的受体的部分具有较高的真核来源，并且配体对培养细胞不是必需的，但是当加入到培养基中时能够进入细胞，将配体加入到培养基中诱导的适当时间点，回收合成的蛋白质。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式