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1. 发明申请

US20050238539A1 Apparatus for automated fresh tissue sectioning 审中-公开
标题翻译：用于自动新鲜组织切片的装置
公开(公告)号：US20050238539A1
公开(公告)日：2005-10-27
申请号：US10816016
申请日：2004-04-01
申请人： Gal Shafirstein , Scott Ferguson , Louis Fink , Paula North , Milton Waner
发明人： Gal Shafirstein , Scott Ferguson , Louis Fink , Paula North , Milton Waner
IPC分类号： B01L3/00 , B23Q15/24 , C12M3/08 , G01N1/04 , G01N1/06
CPC分类号： G01N1/06 , C12M45/07 , G01N2001/045
摘要： An apparatus for sectioning fresh unfixed tissue into very thin layers with preserved tissue architecture, antigenicity, mRNA content, and amenable to 3-D computer reconstruction. An electro-discharge machine (EDM) to accurately slice tissues through electro-dissociation of the tissue without mechanical or thermal damage. The tissue sample is placed on a holder submerged in a cooling bath comprising a liquid such as saline or water to minimize thermal effects and to provide a sink for dissociated ions. A cutting tool is electrically biased with respect to the tissue sample. A computer controlled EDM machine with x-y-z translation stage slices the tissue as defined by a predetermined program. The liquid in the cooling bath may be cooled to minimize tissue heating during cutting. In a preferred embodiment, the cutting tool may use focused RF energy to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analyses by electro-dissociation without mechanical or thermal damage, ultimately allowing high-resolution volumetric reconstruction of gene and protein expression patterns of large tissue specimens.
摘要翻译：将新鲜未固定组织切片成非常薄的装置，其具有保存的组织结构，抗原性，mRNA含量，并适用于3-D计算机重建。一种放电机（EDM），用于通过组织的电解解来精确地切片组织，而没有机械或热损伤。将组织样品放置在浸没在包含液体如盐水或水的冷却浴中的保持器上以最小化热效应并提供用于离解离子的沉淀。切割工具相对于组织样品被电偏置。具有x-y-z平移台的计算机控制的电火花成型机根据预定的程序限定组织切片。冷却浴中的液体可以被冷却以最小化切割期间的组织加热。在优选实施例中，切割工具可以使用聚焦RF能量来产生新鲜组织的连续薄片，用于免疫组织化学和核酸分析，通过电解解而没有机械或热损伤，最终允许基因和蛋白质表达模式的高分辨率体积重建的大组织标本。

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