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1. 发明授权

US4957858A Replicative RNA reporter systems 失效
标题翻译：复制RNA报告系统
公开(公告)号：US4957858A
公开(公告)日：1990-09-18
申请号：US852692
申请日：1988-04-16
申请人： Barbara Chu , Fred R. Kramer , Paul Lizardi , Leslie E. Orgel
发明人： Barbara Chu , Fred R. Kramer , Paul Lizardi , Leslie E. Orgel
IPC分类号： C12N15/09 , C07H21/00 , C07H21/02 , C12Q1/68 , G01N33/532
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/682 , C12Q1/6867 , G01N33/532 , Y10S435/81 , Y10S436/827
摘要： Highly sensitive methods for assaying for biopolymers, such as by immunoassay or nucleic acid probe hybridization assay, and compositions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q beta . The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.
摘要翻译：提供用于测定生物聚合物的高灵敏度方法，例如通过免疫测定或核酸探针杂交测定法，以及用于实施该方法的组合物。该方法使用能够被RNA依赖性RNA聚合酶如噬菌体Qβ的复制酶自动催化复制的RNA作为报告基团。测定方法的高灵敏度是由于可以通过自动催化RNA复制实现的与生物聚合物分析物特异性相关的这种复制RNA的浓度快速指数增加。

2. 发明授权

US4801530A Nucleotide hybridization assay for protozoan parasites 失效
标题翻译：原生动物寄生虫的核苷酸杂交测定
公开(公告)号：US4801530A
公开(公告)日：1989-01-31
申请号：US38959
申请日：1987-04-16
申请人： Nadia Nogueira , Paul Lizardi
发明人： Nadia Nogueira , Paul Lizardi
IPC分类号： C07K14/445 , C12Q1/68 , C12N1/20 , C12N1/10
CPC分类号： C12Q1/6893 , C07K14/445 , Y10S435/947
摘要： The present invention provides a method for detection of protozoan parasites in blood or other specimen from their mammalian hosts. The method comprises nucleic acid hybridization of repetitive nuclear DNA fragments of the parasites. Hybridization probes have been prepared for this purpose by cloning the repetitive nuclear elements that are species-specific in appropriate vectors. The sensitivity of these probes has been increased by further sub-cloning to make them capable of cascade hybridization. The assay is highly specific and sensitive for detection of disease-causing protozoan parasites of the commonly occurring Trypanosomatidae of the genus Leishmania and genus Trypanosoma, as well as for malaria-causing protozoan and other parasitic microorganims of mammals.
摘要翻译：本发明提供了一种从哺乳动物宿主检测血液或其他标本中原生动物寄生虫的方法。该方法包括寄生虫的重复核DNA片段的核酸杂交。已经为此目的制备了杂交探针，通过克隆在适当载体中具有物种特异性的重复核元素。通过进一步亚克隆使其能够进行级联杂交，这些探针的灵敏度得到提高。该测定法对于检测利什曼原虫属和锥虫属常见的锥虫属的致病原生动物寄生虫以及哺乳动物的疟原虫原生动物和其他寄生的微生物，具有高度的特异性和敏感性。

3. 发明申请

US20050069916A1 Ultra-sensitive detection systems 审中-公开
标题翻译：超敏感检测系统
公开(公告)号：US20050069916A1
公开(公告)日：2005-03-31
申请号：US10825568
申请日：2004-04-14
申请人： Brian Chait , Darin Latimer , Paul Lizardi , Eric Kershnar , Jon Morrow , Matthew Roth , Martin Mattessich , Kevin McConnell
发明人： Brian Chait , Darin Latimer , Paul Lizardi , Eric Kershnar , Jon Morrow , Matthew Roth , Martin Mattessich , Kevin McConnell
IPC分类号： G01N27/62 , C07H21/02 , C07H21/04 , C07K1/04 , C07K1/107 , C07K1/13 , C07K7/00 , C07K7/06 , C07K7/08 , C12Q1/68 , G01N27/333 , G01N27/447 , G01N27/64 , G01N33/483 , G01N33/53 , G01N33/566 , G01N33/58 , G01N33/68
CPC分类号： G01N33/6842 , C07K1/047 , C07K1/1077 , C07K1/13 , C07K7/06 , C07K7/08 , G01N33/6803
摘要： Disclosed are compositions and methods for sensitive detection of one or multiple analytes. In general, the methods involve the use of special label components, referred to as reporter signals, that can be associated with, incorporated into, or otherwise linked to the analytes. In some embodiments, the reporter signals can be altered such that the altered forms of different reporter signals can be distinguished from each other. In some embodiments, sets of reporter signals can be used where two or more of the reporter signals in a set have one or more common properties that allow the reporter signals having the common property to be distinguished and/or separated from other molecules lacking the common property. In other embodiments, sets of reporter signal/analyte conjugates can be used where two or more of the reporter signal/analyte conjugates in a set have one or more common properties that allow the reporter signal/analyte conjugates having the common property to be distinguished and/or separated form other molecules lacking the common property. Reporter signals can also be in conjunction with analytes (such as in mixtures of reporter signals and analytes), where no significant physical association between the reporter signals and analytes occurs; or alone, where no analyte is present.
摘要翻译：公开了用于敏感检测一种或多种分析物的组合物和方法。通常，这些方法涉及使用称为报告信号的特殊标记成分，其可以与分析物相关联，并入或以其它方式连接到分析物上。在一些实施方案中，可以改变报道信号，使得不同报道信号的改变形式可以彼此区分。在一些实施方案中，可以使用一组报告信号，其中一组中的两个或更多个报告基因信号具有一个或多个共同性质，其允许具有共同性质的报道信号被区分和/或与其他分子不相同属性。在其它实施方案中，可以使用组的报告信号/分析物缀合物，其中组中的两个或多个报道信号/分析物缀合物具有一个或多个共同性质，其允许区分具有共同性质的报道信号/分析物缀合物， /或分离形成其他缺乏共同性质的分子。记者信号也可以与分析物（例如报告信号和分析物的混合物）结合，其中报告信号和分析物之间不存在明显的物理结合; 或单独存在，其中不存在分析物。

4. 发明授权

US5364760A Replicative RNA reporter systems 失效
标题翻译：复制RNA报告系统
公开(公告)号：US5364760A
公开(公告)日：1994-11-15
申请号：US949766
申请日：1992-09-22
申请人： Barbara Chu , Fred R. Kramer , Paul Lizardi , Leslie E. Orgel
发明人： Barbara Chu , Fred R. Kramer , Paul Lizardi , Leslie E. Orgel
IPC分类号： C12N15/09 , C07H21/00 , C07H21/02 , C12Q1/68 , G01N33/532
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/682 , C12Q1/6867 , G01N33/532 , Y10S435/81 , Y10S436/827
摘要： Highly sensitive methods for assaying for biopolymers, such as by immunoassay or nucleic acid probe hybridization assay, and compositions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q.beta.. The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.
摘要翻译：提供用于测定生物聚合物的高灵敏度方法，例如通过免疫测定或核酸探针杂交测定法，以及用于实施该方法的组合物。该方法使用能够被RNA依赖性RNA聚合酶如噬菌体Qβ的复制酶自动催化复制的RNA作为报告基团。测定方法的高灵敏度是由于可以通过自动催化RNA复制实现的与生物聚合物分析物特异性相关的这种复制RNA的浓度快速指数增加。

5. 发明授权

US4615973A Genetically engineered organisms expressing surface proteins of T. cruzi 失效
标题翻译：表达克氏锥虫表面蛋白的转基因生物
公开(公告)号：US4615973A
公开(公告)日：1986-10-07
申请号：US537798
申请日：1983-09-30
申请人： Paul Lizardi , Nadia Nogueira
发明人： Paul Lizardi , Nadia Nogueira
IPC分类号： C12N15/09 , A61K39/00 , A61K39/005 , C07K14/00 , C07K14/44 , C07K14/705 , C12N1/20 , C12P21/00 , C12Q1/68 , C12R1/19 , G01N33/569 , G01N33/577 , C12N15/00 , C12M1/02
CPC分类号： C07K14/44 , A61K39/00
摘要： Genetically engineered plasmids which express DNA encoding for insect stage specific glycoproteins of Trypanosoma cruzi are disclosed. The glycoproteins offer potential diagnostic utility for the detection of Chagus disease.
摘要翻译：公开了表达编码锥虫属昆虫阶段特异性糖蛋白的DNA的基因工程质粒。糖蛋白提供潜在的诊断效用用于检测查古斯病。

6. 发明授权

US5047522A Isolated repetitive DNA of protozoan parasites 失效
标题翻译：分离的原生动物寄生虫的重复DNA
公开(公告)号：US5047522A
公开(公告)日：1991-09-10
申请号：US217381
申请日：1988-07-11
申请人： Nadia Nogueira , Paul Lizardi
发明人： Nadia Nogueira , Paul Lizardi
IPC分类号： C07K14/445 , C12Q1/68
CPC分类号： C07K14/445 , C12Q1/6893 , Y10T436/143333
摘要： Repetitive DNA sequences of the protozoan parasites T. cruzi and P. falciparum have been isolated. These sequences appear more than once in the parasitic organism. Once isolated, the DNA sequences may be labelled with materials such as chromogens, biotin, or radioactive substances. The sequences may be used, in labelled or unlabelled form, as probes for the parasites.
摘要翻译：已经分离了原生动物寄生虫T. cruzi和恶性疟原虫的重复DNA序列。这些序列在寄生生物中出现不止一次。一旦分离，DNA序列可以用诸如色素，生物素或放射性物质的材料标记。序列可以以标记或未标记的形式用作寄生虫的探针。

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