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1. 发明授权

US5571682A Calibrating and testing immunoassays to minimize interferences 失效
标题翻译：校准和测试免疫测定以尽量减少干扰
公开(公告)号：US5571682A
公开(公告)日：1996-11-05
申请号：US363099
申请日：1994-12-22
申请人： Merrit N. Jacobs , Paul A. Kildal-Brandt , Thomas C. Arter
发明人： Merrit N. Jacobs , Paul A. Kildal-Brandt , Thomas C. Arter
IPC分类号： C12Q1/28 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/581 , Y10S435/962 , Y10S435/97 , Y10S436/805 , Y10S436/815
摘要： A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism. The method features the steps of:a) calibrating an immunoassay of a target analyte by ascertaining the time at which the rate of change in density produced by a first set of the test elements for the target analyte on at least one liquid having a known amount of interferent of choice and a known amount of target analyte, crosses over or minimizes the difference compared to the rate of change in density produced by a second set of test elements substantially identical to the first set of test elements, on a liquid having the known amount of target analyte but a negligible amount of interferent of choice, andb) conducting an assay of a patient sample of unknown amount of the target analyte by reading the rate of change in density in a third set of the test elements substantially identical to the first and second sets, caused by the sample, at a time corresponding to the cross-over time ascertained in step a).
摘要翻译：使用以过氧化物酶和无色染料为特征的干燥载玻片测试元件作为标记机制，减少免疫测定中的血红蛋白偏差等干扰物偏差的方法。该方法的特征在于以下步骤：a）通过确定由至少一种具有已知量的液体的目标分析物的第一组测试元件产生的密度变化率的时间来校准目标分析物的免疫测定的选择干扰物和已知量的目标分析物，与具有基本上与第一组测试元件基本相同的第二组测试元件产生的密度变化率相比，与已知的量的目标分析物交叉或最小化目标分析物的量，但是可选择的干扰物的量可忽略不计，以及b）通过读取第三组测试元件中的密度变化率来进行对未知量的目标分析物的患者样品的测定，其基本上与在对应于步骤a）中确定的交叉时间的时间，由样本引起的第一和第二组。

2. 发明授权

US5257212A Normalizing analyzer systems to a standard analyzer 失效
标题翻译：将分析仪系统归一化为标准分析仪
公开(公告)号：US5257212A
公开(公告)日：1993-10-26
申请号：US836694
申请日：1992-02-18
申请人： Paul A. Kildal-Brandt , Thomas A. Weber , Charles E. Heckler
发明人： Paul A. Kildal-Brandt , Thomas A. Weber , Charles E. Heckler
IPC分类号： G01N35/00 , G01N21/27 , G01N33/483 , G01N33/487 , G06F15/42 , G06F15/20
CPC分类号： G01N21/274
摘要： There is described a method of calibrating an analyzer in the field, using parameters designed for a "standard" analyzer that may be different from the field analyzer. In this way, it is not necessary to use only certain slide elements of a given chemistry on one analyzer, and certain other slide elements of that same chemistry on another analyzer. Instead, the slide elements are made interchangeable by calibration math which allow the field analyzer to convert its raw response R.sub.F into a response R.sub.standard of the selected "standard" analyzer, using the equationR.sub.standard =B.sub.0 +B.sub.1 .multidot.R.sub.F +B.sub.2 .multidot.(R.sub.F).sup.k (I)The coefficients of this equation are established by comparing, at at least 1 different level of response, R.sub.F against R.sub.standard, optionally using an intermediate spline function g.sub.F (R.sub.F) in place of R.sub.F.

3. 发明授权

US5449624A Windowing in rate assays without using initial predicted concentration 失效
标题翻译：在不使用初始预测浓度的情况下进行速率分析
公开(公告)号：US5449624A
公开(公告)日：1995-09-12
申请号：US203081
申请日：1994-02-28
申请人： Paul A. Kildal-Brandt , Thomas A. Weber
发明人： Paul A. Kildal-Brandt , Thomas A. Weber
IPC分类号： G01N21/75 , G01N21/78 , G01N33/536 , G01N33/543 , G01N33/573 , G01N33/94 , G01N35/00
CPC分类号： G01N33/94 , G01N33/54306 , G01N33/573 , Y10T436/112499
摘要： A method of assaying analytes using a rate procedure is described where the change in density over time has a variable rate. The method features the steps of depositing the sample onto a dried slide-like test element, making an initial rate reading during an early time window, using the initial rate readings in a comparison study with rates from known low and high concentration results to predict whether the sample rate will be sufficiently low as to be ascertainable during a later time window or not, and then calculating a rate of reaction and concentration during either the early time window or the early time window with a portion of the later time window, respectively. To avoid the possibility of a consistent bias, the initial rate readings are used directly in the comparison study without first calculating a concentration, by comparing the initial rate reading of a sample against known rates corresponding to samples with low and high concentrations to determine the length of the time window to be used in the rate calculation.
摘要翻译：描述了使用速率程序测定分析物的方法，其中密度随时间的变化具有可变速率。该方法的特征在于将样品沉积在干燥的载玻片状测试元件上，在早期时间窗口中进行初始速率读数，使用来自已知低浓度和高浓度结果的比较研究中的初始速率读数来预测是否采样率将足够低以至于在稍后的时间窗口内可以确定，然后分别在早期时间窗口或早期时间窗口期间分别与稍后的时间窗口的一部分一起计算反应和浓度的速率。为了避免一致偏差的可能性，初始速率读数直接用于比较研究中，而无需首先计算浓度，通过比较样品的初始速率读数与对应于具有低和高浓度样品的已知速率来确定长度的时间窗口用于速率计算。

4. 发明授权

US5126952A Bar coding calibration 失效
标题翻译：条形码校准
公开(公告)号：US5126952A
公开(公告)日：1992-06-30
申请号：US644058
申请日：1991-01-22
申请人： Paul A. Kildal-Brandt , Thomas A. Weber
发明人： Paul A. Kildal-Brandt , Thomas A. Weber
IPC分类号： G01N33/49 , G01N33/00 , G01N33/487 , G06K7/00 , G06K19/06
CPC分类号： G01N33/48771
摘要： There is described a method of barcoding data needed to determine a calibration curve for a test element in an analyzer. The process follows the steps of:a) ascertaining by statistical analysis the ranges of values for R that are possible for three given concentration values C.sub.1, C.sub.2 and C.sub.3, for a given assay, and assigning a high value H and a low value L for these ranges;b) calculating for a given lot of the given assay, a calibration curve that correlates the analyzer response to the concentration,c) determining the analyzer response R.sub.1, R.sub.2 and R.sub.3 from said calibration curve, that corresponds to the C.sub.1, C.sub.2 and C.sub.3 values;d) calculating the bar code value B.sub.i for R.sub.i of each of these R.sub.1, R.sub.2 and R.sub.3 from the equation:B.sub.i =(10.sup.n -1)(R.sub.i -L.sub.i)/(H.sub.i -L.sub.i) (2)where R.sub.i is R.sub.1, R.sub.2 or R.sub.3, and L.sub.i and H.sub.i are the corresponding L and H values for that R.sub.i ;e) rounding B.sub.i to the nearest integer; andf) supplying this value of B.sub.i for each of R.sub.i =R.sub.1, R.sub.2 or R.sub.3 in bar code form, using n-digit decimal numbers,so that only three sets of (10.sup.n) possibilities are needed to accurately pass along data corresponding to the calibration coefficients even though each of the three coefficients can vary by more than that which can be specified using 10.sup.n digits.

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