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1. 发明申请

WO2010040480A1 SMALL SCALE SHAKER FLASK CULTIVATION 审中-公开
标题翻译：小规模的滑雪板快速启动
公开(公告)号：WO2010040480A1
公开(公告)日：2010-04-15
申请号：PCT/EP2009/007069
申请日：2009-10-02
申请人： F. HOFFMANN-LA ROCHE AG , KREUZMANN, Jan , PUSKEILER, Robert , SCHUSTER, Caroline
发明人： KREUZMANN, Jan , PUSKEILER, Robert , SCHUSTER, Caroline
IPC分类号： C12P21/00 , C12M1/36
CPC分类号： C12M41/36 , C12M35/04 , C12M41/42
摘要： The current invention reports a method for the cultivation of mammalian cells expressing a heterologous polypeptide, wherein a) the cultivation is performed in a cultivation vessel of a total volume of 25 ml to 3000 ml, b) the cultivation vessel contains a cultivation medium and an inside gas phase above the cultivation medium, c) the cultivation vessel contains a membrane or a cap or a lid separating said inside gas phase from the outside gas phase, d) in the cultivation vessel the agitation of the cultivation medium is performed by a mechanical movement of the entire cultivation vessel, e) during the cultivation the mechanical movement speed is changed depending on the viable cell density in the cultivation medium or it is changed depending on the oxygen partial pressure in the cultivation medium, f) the pH value of the cultivation medium is adjusted via the carbon dioxide concentration in the gas phase outside of the cultivation vessel, whereby the course of the viable cell density or the volumetric yield of the heterologous polypeptide expressed by the mammalian cell are similar to those obtained with a mechanically stirred cultivation vessel of a total volume of 1,000 1 to 25.000 1.
摘要翻译：本发明报道了用于培养表达异源多肽的哺乳动物细胞的方法，其中a）在总体积为25ml至3000ml的培养容器中进行培养，b）培养容器含有培养基和在培养基上方的内部气相中，c）培养容器含有将内部气相与外部气相分离的膜或盖或盖，d）在培养容器中，培养介质的搅拌通过机械整个培养容器的运动，e）在培养期间机械运动速度根据培养基中的活细胞密度而改变，或根据培养基中的氧分压而变化，f）通过培养容器外的气相中的二氧化碳浓度调节培养基，由此活细胞密度的变化或由哺乳动物细胞表达的异源多肽的体积产量与用总体积为1,000 1至25.000 1的机械搅拌培养容器获得的类似。

2. 发明申请

WO2004058935A3 DEVICE AND METHOD FOR PARALLEL, AUTOMATED CULTIVATION OF CELLS IN TECHNICAL CONDITIONS 审中-公开
标题翻译：设备和方法的同时，培育AUTOMATED细胞技术条件
公开(公告)号：WO2004058935A3
公开(公告)日：2005-09-22
申请号：PCT/EP0314752
申请日：2003-12-22
申请人： UNIV MUENCHEN TECH , PUSKEILER ROBERT , WEUSTER-BOTZ DIRK , ZACHER KARL-HEINZ
发明人： PUSKEILER ROBERT , WEUSTER-BOTZ DIRK , ZACHER KARL-HEINZ
IPC分类号： B01F3/04 , B01F13/06 , B01F13/08 , B01F15/00 , B01F15/06 , C12M1/02 , C12M1/18 , C12M1/32
CPC分类号： C12M27/20 , B01F3/04531 , B01F3/04539 , B01F13/065 , B01F13/0818 , B01F13/0827 , B01F15/00071 , B01F15/0022 , B01F15/00915 , B01F15/065 , B01F2003/04567 , B01F2003/04645 , B01F2215/0037 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481 , C12M23/42 , C12M27/02 , C12M41/00 , C12M41/48
摘要： The invention relates to a device for the cultivation of cells in liquid columns measured on a millimetre scale. Said device comprises a container for receiving a liquid culture suspension of the cells, a mixing element for mixing the culture suspension in the container around a mixing axis. The container and/or the mixing element are configured in such a manner that the speed of the flow is modified either locally and/or temporally along a streamline or flow line around the mixing axis.
摘要翻译：本发明涉及一种设备，用于在与容器的规模毫升液柱培养细胞，用于接收所述细胞的液体培养物悬浮液，Rührörgan在容器搅拌培养悬浮液进行搅拌轴。容器和/或搅拌元件被形成为使得流速沿着围绕轴线搅拌流线型路径或线路在空间和/或时间而变化。

3. 发明申请

WO2004058935A2 VORRICHTUNG UND VERFAHREN ZUR PARALLELEN, AUTOMATISIERTEN KULTIVIERUNG VON ZELLEN UNTER TECHNISCHEN BEDINGUNGEN 审中-公开
标题翻译：设备和方法的同时，培育AUTOMATED细胞技术条件
公开(公告)号：WO2004058935A2
公开(公告)日：2004-07-15
申请号：PCT/EP2003/014752
申请日：2003-12-22
申请人： TECHNISCHE UNIVERSITÄT MÜNCHEN , PUSKEILER, Robert , WEUSTER-BOTZ, Dirk , ZACHER, Karl-Heinz
发明人： PUSKEILER, Robert , WEUSTER-BOTZ, Dirk , ZACHER, Karl-Heinz
IPC分类号： C12M1/00
CPC分类号： C12M27/20 , B01F3/04531 , B01F3/04539 , B01F13/065 , B01F13/0818 , B01F13/0827 , B01F15/00071 , B01F15/0022 , B01F15/00915 , B01F15/065 , B01F2003/04567 , B01F2003/04645 , B01F2215/0037 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481 , C12M23/42 , C12M27/02 , C12M41/00 , C12M41/48
摘要： Die Erfindung betrifft eine Vorrichtung zur Kultivierung von Zellen in Flüssigkeitssäulen im Milliliter-Massstab mit einem Behälter zur Aufnahme einer flüssigen Kultursuspension der Zellen, einem Rührörgan zum Rühren der Kultursuspension in dem Behälter um eine Rührachse. Der Behälter und/oder das Rührorgan sind derart ausgebildet, dass die Strömungsgeschwindigkeit sich längs einer Stromlinie oder Bahnlinie um die Rührachse örtlich und/oder zeitlich ändert.
摘要翻译：本发明涉及一种设备，用于在与容器的规模毫升液柱培养细胞，用于接收所述细胞的液体培养物悬浮液，Rührörgan在容器搅拌培养悬浮液进行搅拌轴。容器和/或搅拌元件被形成为使得流速沿着围绕轴线搅拌流线型路径或线路在空间和/或时间而变化。

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